Nitric oxide and malondialdehyde levels in plasma and tissue of psoriasis patients

Aktürk, Aysun Şikar; Ozdoğan, Haci Kahya; Bayramgürler, Dilek; Çekmen, Mustafa; Bilen, Nurhan; Kiran, Ravi

doi:10.1111/j.1468-3083.2011.04164.x

Cited by 55 publications

(36 citation statements)

References 22 publications

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“…We also demonstrated that nitric oxide is important in the control of wound healing [10]. Nitric oxide also controls keratinocyte proliferation [11], while in human skin, it plays a key role in regulating cellular responses in diseases states such as psoriasis [12, 13], as well as to infections, heat, ultraviolet light and wounding [14–16]. The aim of the present studies was to analyze the response of keratinocytes to the nitrofatty acids 9-nitrooleic acid (9-NO) and 10-nitrooleic acid (10-NO).…”

Section: Introductionmentioning

confidence: 99%

Regulation of keratinocyte expression of stress proteins and antioxidants by the electrophilic nitrofatty acids 9- and 10-nitrooleic acid

Zheng

Heck

Black

et al. 2014

Free Radical Biology and Medicine

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Nitric oxide and various by-products including nitrite contribute to tissue injury by forming novel intermediates via redox-mediated nitration reactions. Nitration of unsaturated fatty acids generates electrophilic nitrofatty acids such as 9-nitrooleic acid (9-NO) and 10-nitrooleic acid (10-NO), which are known to initiate intracellular signaling pathways. In these studies, we characterized nitrofatty acid-induced signaling and stress protein expression in mouse keratinocytes. Treatment of keratinocytes with 5–25 μM 9-NO or 10-NO for 6 h upregulated mRNA expression of heat shock proteins (hsp) 27 and 70; primary antioxidants, heme oxygenase-1 (HO-1) and catalase; secondary antioxidants glutathione-S-transferase (GST) A1/2, GSTA3 and GSTA4; and Cox-2, a key enzyme in prostaglandin biosynthesis. The greatest responses were evident with HO-1, hsp27 and hsp70. In keratinocytes, 9-NO activated JNK and p38 MAP kinases. JNK inhibition suppressed 9-NO induced HO-1, hsp27 and hsp70 mRNA and protein expression, while p38 MAP kinase inhibition suppressed HO-1. In contrast, inhibition of constitutive expression of ERK1/2 suppressed only hsp70 indicating that 9-NO modulates expression of stress proteins by distinct mechanisms. 9-NO and10-NO also upregulated expression of caveolin-1, the major structural component of caveolae. Western blot analysis of caveolar membrane fractions isolated by sucrose density centrifugation revealed that HO-1, hsp27 and hsp70 were localized within caveolae following nitrofatty acid treatment of keratinocytes suggesting a link between induction of stress response proteins and caveolin-1 expression. These data indicate that nitrofatty acids are effective signaling molecules in keratinocytes. Moreover, caveolae appear to be important in the localization of stress proteins in response to nitrofatty acids.

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Section: Introductionmentioning

confidence: 99%

Regulation of keratinocyte expression of stress proteins and antioxidants by the electrophilic nitrofatty acids 9- and 10-nitrooleic acid

Zheng

Heck

Black

et al. 2014

Free Radical Biology and Medicine

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“…Mindezek hátterében a psoriasisos plakkokban már a betegség korai fázisában jelen lévő polimorfonukleáris fehérvérsejtek szabadgyök-terhelést okozó hatása állhat. Ezt a feltételezést erősíti az az eredmény, miszerint pozitív korreláció van a psoriasis súlyossága és az MDA koncentrációja között [19,[27][28][29].…”

Section: Malonilaldehid éS a Psoriasisunclassified

A psoriasis és az oxidatív stressz

Péter¹,

Jagicza²,

Ajtay

et al. 2016

Orvosi Hetilap

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A jelen tudományos közleményt a szerzők a Pécsi Tudományegyetem alapításának 650. évfordulója emlékének szentelik.A psoriasis hazánkban és világviszonylatban is a leggyakrabban előforduló bőrbetegségek egyike. Jelentőségét hangsú-lyozza a gyakran élethossziglan fennálló plakkok okozta fájdalom, mozgáskorlátozottság és a pszichés terhelés életmi-nőségre gyakorolt hatása. Szövődményeit tekintve a nemegyszer maradandó károsodásokat okozó arthritis psoriaticán túl, a psoriasisos betegek esetében nagyobb cardiovascularis kockázattal kell számolni, gyakrabban alakulnak ki gyulladásos bélbetegségek és bizonyos daganatok. Az exogén és endogén tényezők okozta oxidatív stressz és a genetikai prediszpozíció hozzájárulhat a keratinocyták hiperproliferációjához és abnormális differenciálódásához, ezáltal a psoriasis kialakulásához, illetve fenntartásához. A nagyfokú oxidatív stressz felelős lehet továbbá a psoriasis szövődménye-inek megjelenéséért. Jelen közleményben, rövid patofiziológiai áttekintés után, néhány jól ismert biomarker (aszimmetrikus dimetilarginin, malonilaldehid, szuperoxid dizmutáz, kataláz) segítségével a szerzők bemutatják az oxidatív stressz szerepét a psoriasis és szövődményeinek kialakulásában. Orv. Hetil., 2016, 157(45), 1781-1785. Kulcsszavak: psoriasis, biomarker, oxidatív stressz Psoriasis and oxidative stressPsoriasis is among the most common dermatological diseases worldwide. Its significance is emphasized by adverse effects on quality of life, caused by chronic pain, physical and psychical disability due to psoriatic plaques. Besides the development of psoriatic arthritis, which often causes permanent joint damage, former studies revealed an increased risk of inflammatory bowel disease, cardiovascular disease and certain types of cancer. Genetic predisposition and oxidative stress caused by exogenous and endogenous factors can contribute to abnormal differentiation and hyperproliferation of keratinocytes, accordingly the development and maintenance of psoriasis. Moreover, excessive oxidative stress can be responsible for the onset of psoriasis complications. After a brief pathophysiological summary the authors discuss the role of oxidative stress in the development of psoriasis and its complications through several well studied biomarkers (asymmetric dimethylarginine, malondialdehyde, superoxide dismutase, catalase). Keywords ÖSSZEFOGLALÓ KÖZLEMÉNYRövidítések ADMA = aszimmetrikus dimetilarginin; BB = béta-blokkoló; BMI = body mass index; CARD-14 = caspase recruitment domain family member 14, CAT = catalase; CV = cardiovascularis; DLQI = Dermatology Life Quality Index, IF = interferon; IL12B = interleukin 12B; MDA = malonilaldehid; MHC = major histocompatibility complex; Li = lítium; MI = myocardialis infarctus; NFκB = nuclear factor kappa BVZ; NSAID = nem szteroid gyulladáscsökkentő; PASI = Psoriasis Area Severity Index; PSORS = psoriasis-susceptibility; PsA = arthritis psoriatica; SOD = szuperoxid dizmutáz; TNF = tumor necrosis factor A psoriasis a leggyakrabban előforduló bőrbetegségek egyike...

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“…Though the positive outcomes associated with JYG can be observed clinically, the underlying mechanism for those outcomes is not well understood. Recent studies have suggested that nitric oxide (NO) and malondialdehyde (MDA) may play a part in the pathogenesis of various skin diseases, including psoriasis [5, 6]. Specifically, studies have shown that increased NO and MDA contribute to psoriasis activity [5].…”

Section: Introductionmentioning

confidence: 99%

Effects of Chinese Formula Jueyin Granules on Psoriasis in an Animal Model

Jiang

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Although Traditional Chinese medicine (TCM) is known to be effective for psoriasis patients, the responsible mechanisms still remain poorly understood. In this study, we aimed to evaluate the effect of one formula, named Jueyin granules (JYG) in the mouse model of the vaginal epithelium and tail epidermis. Additionally, we also determined the anti-inflammatory effects of JYG in an imiquimod- (IMQ-) induced psoriasis-like skin mouse model. Our results show that JYG can attenuate the IMQ-induced psoriasis-like inflammation, accompanied with increased epidermal hyperplasia. We also measured estrogenic stage mitosis of vaginal epithelial cells and the formation of granular cell layers in male mouse tails per 100 scales, as well as the tissue nitric oxide (NO) and malondialdehyde (MDA) levels using the ELISA method. The results suggest that JYG significantly inhibited mitosis in mouse vaginal epithelial cells, promoted the formation of the squamous epidermal granular layer in mice tails, and reduced the levels of NO and MDA in an imiquimod-induced psoriasis-like skin mouse model after 14 d (P < 0.05). These results demonstrate that JYG might be an effective clinical treatment for psoriasis and the effects may be related to inhibited keratinocytes proliferation, improved parakeratotic epidermal cells, and reduced expression of NO and MDA.

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Nitric oxide and malondialdehyde levels in plasma and tissue of psoriasis patients

Abstract: NO and MDA levels are elevated in psoriasis patients, which may indicate that oxidative stress plays an important role in the aetiopathogenesis of psoriasis.

Cited by 55 publications

References 22 publications

Regulation of keratinocyte expression of stress proteins and antioxidants by the electrophilic nitrofatty acids 9- and 10-nitrooleic acid

Regulation of keratinocyte expression of stress proteins and antioxidants by the electrophilic nitrofatty acids 9- and 10-nitrooleic acid

A psoriasis és az oxidatív stressz

Effects of Chinese Formula Jueyin Granules on Psoriasis in an Animal Model

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