Nitric oxide and cyclooxygenase may participate in the analgesic and anti-inflammatory effect of the cucurbitacins fraction from Wilbrandia ebracteata

Peters, Rodrigo Rebelo; Krepsky, Patrícia Baier; Siqueira-Junior, J.M.; Rocha, José Geraldo; Bezerra, Mirna Marques; Ribeiro, Ronaldo de Albuquerque; Brum‐Fernandes, Artur J. de; Farías, Mareni Rocha; Rocha, Francisco Airton Castro da; Ribeiro‐do‐Valle, Rosa Maria

doi:10.1016/s0024-3205(03)00602-7

Cited by 26 publications

(4 citation statements)

References 37 publications

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“…Cucurbitacin B (CuB), a member of the cucurbitacins, is extracted from Cucurbitaceae plants that have long been used in oriental medicines for their anti-inflammatory, antidiabetic and hepatoprotective effects [1,2,3]. Recently, accumulating evidence has demonstrated that CuB and its relatives inhibit the proliferation of many cancerous cells both in vitro and in xenografted tumor models, including breast cancer, glioblastoma multiforme, myeloid leukemia, pancreatic cancer, laryngeal cancer, hepatocellular carcinoma and melanoma cells [3,4,5,6,7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Cucurbitacin B Induces Cell Cycle Arrest, Apoptosis and Autophagy Associated with G Actin Reduction and Persistent Activation of Cofilin in Jurkat Cells

et al. 2012

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Aim: The present study aimed to explore the antitumor effect and action mechanism of cucurbitacin B (CuB) on human T-cell leukemia Jurkat cells. Methods: Cell proliferation was measured by the MTS assay. Cell cycle distribution, mitochondrial membrane potential and annexin V staining were analyzed using flow cytometry. Western blotting was used to determine the levels of apoptosis- and autophagy-related proteins. Results: CuB inhibited the proliferation of Jurkat cells in a dose-dependent manner and induced G₂/M phase arrest as well as formation of tetraploid cells. Accompanied with these effects, the actin dynamics was disrupted, and cofilin, a key regulator of actin dynamics, was persistently activated (dephosphorylated). Although CuB induced around 10% cells undergoing apoptosis, most of the cells were alive after CuB treatment for 24 h. Induction of autophagy was also evident by accumulation of LC3-II. CuB-induced autophagy seemed to be a prosurvival response, since suppression of CuB-induced autophagy significantly increased the activation of caspase-3. Conclusion: Our results demonstrated that CuB exhibited antitumor activity in Jurkat cells through induction of cell cycle arrest and apoptosis which was at least partly due to the disruption of actin dynamics.

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Section: Introductionmentioning

confidence: 99%

Cucurbitacin B Induces Cell Cycle Arrest, Apoptosis and Autophagy Associated with G Actin Reduction and Persistent Activation of Cofilin in Jurkat Cells

et al. 2012

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“…Peters et al (2003) [ 33 ] observed that the dichloromethane extract of Wilbrandia ebracteate (Curubitaceae) significantly inhibited inflammatory parameters in experimental models of in vivo and in vitro inflammation. An analysis of the chemical composition of the extract via HPLC indicated the presence of cucurbitacins in the extract.…”

Section: Resultsmentioning

confidence: 99%

Anti-Tumor and Anti-Inflammatory Activity In Vivo of Apodanthera congestiflora Cogn. (Cucurbitaceae)

et al. 2021

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This work aimed to carry out a study of Apodanthera congestiflora by investigating its chemical composition and pharmacological potential. From the dichloromethane phase (Dic-Ac) of the A. congestiflora stems, three compounds were identified: cayaponoside C5b (Ac-1), cabenoside C (Ac-2) and fevicordin C2 glucoside (Ac-3), being last identified for the first time as a natural product. These compounds were obtained by chromatographic methods and their structures were elucidated by means of spectroscopic analysis of IR, MS and NMR. In the quantification of Dic-Ac, it was possible to observe the presence of 7% of cayaponoside C5b. Dic-Ac showed significant toxicity for in vivo tests, with macroscopic and biochemical changes. The anti-inflammatory activity of Dic-Ac was investigated using the paw edema model. A decrease in inflammatory signs was observed in the first 5 h and the most effective dose in reducing edema with was 7.5 mg kg−1 (66.6%). Anti-tumor activity of Dic-Ac was evaluated by Ehrlich’s carcinoma model, which showed inhibition rate of 78.46% at 15 mg kg−1 dosage. The phytochemical investigation, together with the biological tests carried out in this study, demonstrated that A. congestiflora is a promising species in the search for therapeutics, since it contains substances with high pharmacological potential in its composition.

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“…As anti-inflammatory agents, cucurbitacins have been shown to inhibit cyclooxygenase (COX) enzymes (Jayaprakasam et al 2003;Peters et al 1997Peters et al , 1999Peters et al , 2003Siqueira et al 2007;Yesilada et al1998). The COX enzymes are involved in the production of prostanoids (such as prostaglandins, thromboxanes, and prostacyclins), biological mediators of inflammation, nociceptive, vasoconstriction, and anaphylactic reactions in animals.…”

Section: Discussionmentioning

confidence: 99%

The Use of Marah Macrocarpus by the Prehistoric Indians of Coastal Southern California

Martin¹

2009

Journal of Ethnobiology

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A review of the available data from macrobotanical analyses conducted on prehistoric archaeological sites found in coastal southern California indicates the ubiquitous nature of carbonized Marah macrocarpus (Greene) Greene (wild cucumber) seed coat fragments. Their recovery from cultural deposits in carbonized form indicates prehistoric use; however, all parts of the plant are toxic, and plants that typically function in medicinal or ritualistic roles are rarely recovered as part of the macrobotanical assemblage. In an attempt to shed some light on the possible prehistoric uses of wild cucumber by the native peoples of coastal southern California, this paper examines the current distribution of Marah, the archaeological occurrence of M. macrocarpus, as well as pertinent biochemical and morphological characteristics of the plant, particularly the seed. Finally, ethnobotanical accounts of the use of Marah by the native peoples of the Pacific west are discussed in light of current research on the physical properties and biological activities of various compounds found in Marah. This information is offered as a guide for future archaeological investigations of the possible uses of the taxon by the prehistoric Indians of coastal southern California and elsewhere.

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Nitric oxide and cyclooxygenase may participate in the analgesic and anti-inflammatory effect of the cucurbitacins fraction from Wilbrandia ebracteata

Cited by 26 publications

References 37 publications

Cucurbitacin B Induces Cell Cycle Arrest, Apoptosis and Autophagy Associated with G Actin Reduction and Persistent Activation of Cofilin in Jurkat Cells

Cucurbitacin B Induces Cell Cycle Arrest, Apoptosis and Autophagy Associated with G Actin Reduction and Persistent Activation of Cofilin in Jurkat Cells

Anti-Tumor and Anti-Inflammatory Activity In Vivo of Apodanthera congestiflora Cogn. (Cucurbitaceae)

The Use of Marah Macrocarpus by the Prehistoric Indians of Coastal Southern California

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