Nitric oxide and abscisic acid protects against PEG-induced drought stress differentially in Brassica genotypes by combining the role of stress modulators, markers and antioxidants

Sahay, Seema; Khan, E.; Gupta, Meetu

doi:10.1016/j.niox.2019.05.005

Cited by 46 publications

(24 citation statements)

References 73 publications

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“…(2) higher NO synthesis occurs when a plant perceives the stress condition either due to oxidative or nitro-oxidative stress which was not exhibited by 100 µM SNP-treated roots in the present study. The result was validated/supported by our previous finding that an increase of spectrophotometrically determined NO content and ABA content under PEG treatment, with more increase under PEG-combined with NO and/or ABA treatments in B. juncea 28 , 29 . Our result was also supported by the study of Cao et al 30 , who observed that NO production level was higher in SNP + PEG treated roots of rice seedlings.…”

Section: Discussionsupporting

confidence: 84%

“…The maximum increase in growth biomass was noted in seedlings supplied with PEG + NO compared to PEG + ABA treatment. This is probably linked to more accumulation of NO and its signaling cascade role leading to the development of root hairs, thereby increased root surface area, improved water uptake i.e., leaf relative water content, (data is shown in our previous study 28 ) and other nutrients, as evident from increased levels of macro-micronutrients and plant growth in the present study. The role of exogenous NO and ABA application to enhance different growth parameters has also been demonstrated in many crops under drought stress 31 – 33 .…”

Section: Discussionsupporting

confidence: 78%

“…The endogenously generated NO content may have contributed to improve the plant’s capacity to absorb more nutrients from the growth medium mediated by its scavenging interaction with ROS or by stimulating the antioxidant system, as evidenced from lower O 2 ·− , H 2 O 2 , and MDA, and higher enzymatic and non-enzymatic antioxidants activities in B. juncea cv. Varuna in our previous study 28 . Furthermore, it has been reported that deficiency of nutrients causes endogenous NO synthesis and degradation rates to trigger responses associated to stress acclimation 57 , 58 .…”

Section: Discussionmentioning

confidence: 65%

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Root NRT, NiR, AMT, GS, GOGAT and GDH expression levels reveal NO and ABA mediated drought tolerance in Brassica juncea L.

Sahay

Robledo-Arratia

Głowacka

et al. 2021

Sci Rep

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Little is known about the interactive effects of exogenous nitric oxide (NO) and abscisic acid (ABA) on nitrogen (N) metabolism and related changes at molecular and biochemical levels under drought stress. The present study highlights the independent and combined effect of NO and ABA (grouped as “nitrate agonists”) on expression profiles of representative key genes known to be involved in N-uptake and assimilation, together with proline metabolism, N–NO metabolism enzyme’s activity and nutrient content in polyethylene glycol (PEG) treated roots of Indian mustard (B. juncea cv. Varuna). Here we report that PEG mediated drought stress negatively inhibited growth performance, as manifested by reduced biomass (fresh and dry weight) production. Total N content and other nitrogenous compounds (NO3−, NO2−) were decreased; however, NH4+, NH4+/ NO3− ratio and total free amino acids content were increased. These results were positively correlated with the PEG induced changes in expression of genes and enzymes involved in N-uptake and assimilation. Also, PEG supply lowered the content of macro- and micro-nutrients but proline level and the activity of ∆1-pyrroline-5-carboxylate synthetase increased indicating increased oxidative stress. However, all these responses were reversed upon the exogenous application of nitrate agonists (PEG + NO, PEG + NO + ABA, and PEG + ABA) where NO containing nitrate agonist treatment i.e. PEG + NO was significantly more effective than PEG + ABA in alleviating drought stress. Further, increases in activities of L-arginine dependent NOS-like enzyme and S-nitrosoglutathione reductase were observed under nitrate agonist treatments. This indicates that the balanced endogenous change in NO and ABA levels together during synthesis and degradation of NO mitigated the oxidative stress in Indian mustard seedlings. Overall, our results reveal that NO independently or together with ABA may contribute to improved crop growth and productivity under drought stress.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 65%

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Root NRT, NiR, AMT, GS, GOGAT and GDH expression levels reveal NO and ABA mediated drought tolerance in Brassica juncea L.

Sahay

Robledo-Arratia

Głowacka

et al. 2021

Sci Rep

Self Cite

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“…At the physiological level, drought stress reduces stomatal conductance and increases transpiration rate [23]. This causes an unbalanced transpiration rate and results in low leaf relative water content (LRWC) [53]. Water potential, water use efficiency, and hydraulic conductivity in drought-stressed plants are remarkably reduced, whereas leaf venation density is increased, indicating that water deficit negatively interferes with the water transportation in leaves [37].…”

Section: Impacts Of Drought On Plantsmentioning

confidence: 99%

“…Photosynthesis, an important biological process for plant survival, is significantly influenced by drought. These include the reduction of leaf carbon dioxide assimilation, ribulose-1,5-bisphosphate (RuBP) regeneration [22], chlorophyll and carotenoid contents, and accessory pigments [53].…”

Section: Impacts Of Drought On Plantsmentioning

confidence: 99%

Plant Nitric Oxide Signaling under Drought Stress

Lau

Hamdan

Pua

et al. 2021

Plants

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Water deficit caused by drought is a significant threat to crop growth and production. Nitric oxide (NO), a water- and lipid-soluble free radical, plays an important role in cytoprotection. Apart from a few studies supporting the role of NO in drought responses, little is known about this pivotal molecular amendment in the regulation of abiotic stress signaling. In this review, we highlight the knowledge gaps in NO roles under drought stress and the technical challenges underlying NO detection and measurements, and we provide recommendations regarding potential avenues for future investigation. The modulation of NO production to alleviate abiotic stress disturbances in higher plants highlights the potential of genetic manipulation to influence NO metabolism as a tool with which plant fitness can be improved under adverse growth conditions.

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