Nitric oxide, 8-hydroxydeoxyguanosine, and total antioxidant capacity in human seminal plasma of infertile men and their relationship with sperm parameters

Abstract

Objective: Oxidative stress plays a key role in the pathogenesis of male infertility. But, the adverse effects of oxidative biomarkers on sperm quality remain unclear. This study aimed to investigate the levels of nitric oxide (NO), 8-hydroxydesoxyguanosine (8-OHdG), and total antioxidant capacity (TAC) oxidative biomarkers in seminal plasma and their relationship with sperm parameters.Methods: A total of 77 volunteers participated in the study, including fertile (n=40) and infertile men (n=37). NO, 8-OHdG, and TAC levels were measured using the ferric reducing ability of plasma, Griess reagent method and an enzyme-linked immunosorbent assay kit, respectively.Results: The mean values of sperm parameters in the infertile group were significantly lower than those in the fertile group (<i>p</i><0.001). The mean 8-OHdG in the seminal plasma of infertile men was significantly higher (<i>p</i>=0.013) than those of controls, while the mean TAC was significantly lower (<i>p</i>=0.046). There was no significant difference in NO level between the two groups. The elevated seminal 8-OHdG levels were negatively correlated with semen volume, total sperm counts and morphology (<i>p</i><0.001, <i>p</i>=0.001 and <i>p</i>=0.052, respectively). NO levels were negatively correlated with semen volume, total sperm counts and morphology (<i>p</i>=0.014, <i>p</i>=0.020 and <i>p</i>=0.060, respectively). Positive correlations between TAC and both sperm count and morphology (<i>p</i>=0.043 and <i>p</i>=0.025, respectively) were also found.Conclusion: These results suggested that increased levels of NO and 8-OHdG in seminal plasma could have a negative effect on sperm function by inducing damage to the sperm DNA hence their fertility potentials. Therefore, these biomarkers can be useful in the diagnosis and treatment of male infertility.

Proinflammatory and Oxidative Stress States Induced by Human Papillomavirus and Chlamydia trachomatis Coinfection Affect Sperm Quality in Asymptomatic Infertile Men

“…Infection only with CT may generate fewer RONS, which also induces less LPO probably because antioxidant systems are still active, increasing TAC levels. However, these levels are similar to those found in other infertile populations [ 27 ] but without seminal HPV, CT, or coinfection, then other factors such as lifestyle choices (tobacco and alcohol consumption) and environmental factors trigger testicular sources of RONS [ 11 , 26 ], which could stimulate OS in CT infection. Moreover, seminal TAC levels were significantly lower in HPV infection or CT coinfection, resembling other infertile male populations [ 27 ], which may decrease the percentage of motile A and non-progressive motility (% motile A + B).…”

“…However, these levels are similar to those found in other infertile populations [ 27 ] but without seminal HPV, CT, or coinfection, then other factors such as lifestyle choices (tobacco and alcohol consumption) and environmental factors trigger testicular sources of RONS [ 11 , 26 ], which could stimulate OS in CT infection. Moreover, seminal TAC levels were significantly lower in HPV infection or CT coinfection, resembling other infertile male populations [ 27 ], which may decrease the percentage of motile A and non-progressive motility (% motile A + B). Gholinezhad et al found a lower percentage of motility in infertile men compared to fertile men, although with typical values of this sperm parameter according to the WHO [ 27 ].…”

“…Moreover, seminal TAC levels were significantly lower in HPV infection or CT coinfection, resembling other infertile male populations [ 27 ], which may decrease the percentage of motile A and non-progressive motility (% motile A + B). Gholinezhad et al found a lower percentage of motility in infertile men compared to fertile men, although with typical values of this sperm parameter according to the WHO [ 27 ].…”

Effects of hazelnut supplemented diet on doxorubicin‐induced damage of reproductive system in male rats

“…For example, arginine, an amino acid present in hazelnut, is required for normal spermatogenesis; a precursor for polyamines found in the male reproductive system, especially the epididymis and affects cell growth, differentiation, and sperm motility. It is also a precursor of antioxidant nitric oxide (NO) that regulates spermatogenesis, sperm hyperactivation, sperm motility, maturation, acrosome reaction, capacitation, and sperm fertilization (Gholinezhad et al., 2020; Özer Kaya et al., 2020). On the other hand, a main mineral in hazelnut, potassium, is needed to maintain sperm osmotic pressure, volume, and motility (Vílchez et al., 2017).…”

The Impact of Oxidative Stress in Male Infertility

“…Patients with asthenozoospermia show enhanced mtDNA copy number and reduced mtDNA integrity that are associated with higher ROS generation (Bonanno et al, 2016). Accordingly, other reports underlined the significant association between NO and 8-OHdG levels and semen parameters abnormalities (Gholinezhad et al, 2020), supporting redox status assessment for helping male infertility diagnosis and monitoring.…”