Nitration Enhances the Allergenic Potential of Proteins

Gruijthuijsen, YK; Grieshuber, Ines; Stöcklinger, Angelika; Tischler, U; Fehrenbach, T; Weller, Michael G.; Vogel, Lothar; Vieths, Stefan; Pöschl, Ulrich; Duschl, Albert

doi:10.1159/000095296

Cited by 117 publications

(120 citation statements)

References 49 publications

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“…The physiological role of nitrated albumin is complex, beneficial, because it increases vascular permeability, which is considered a critical mechanism for clearing NO and ONOO 2 (and their derivatized products) from the circulation, and detrimental, because protein nitration is able to enhance allergic responses (32,33). Processes which contribute to increase nitrated albumin in blood have no clear consequences yet.…”

Section: Discussionmentioning

confidence: 99%

First evidence for an LDL‐ and HDL‐associated nitratase activity that denitrates albumin‐bound nitrotyrosine—Physiological consequences

Léger

Torres‐Rasgado²,

Fouret³

et al. 2007

IUBMB Life

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SummaryIn the systemic circulation, LDL occurs in the form of a weakly nitrated LDL-albumin complex (LAC). The question here is whether LAC (or HDL) is able to denitrate the albumin-bound 3-NO 2 -tyrosine (3NT). Nitrated albumin was incubated in the presence of lipoprotein fraction (LPF) to be tested, with or without Ca 21 . After precipitation and centrifugation, supernatants (SNs) and protein pellets (PP) were collected. HCl proteolysis was carried out with deuterated 3NT as an internal standard, and amino acids were derivatized for GC-MS analysis, whereas SNs were used for NO 2 2 /NO 3 2 -fluorimetric assays. A loss of 3NT, higher with albumin-low LDL than with albumin-rich LDL or HDL, was found in PP only in the presence of Ca 21 . c-Tocopherol loading of LPF inhibited 3NT loss. 3NT loss was found for the first time to be stoichiometrically equivalent to NO 3 2 , proving that the 3NT loss must be ascribed to a 3NT-denitrating nitratase activity. 3NT loss and NO 3 2 production that clearly cannot be attributed to PON-1 were impaired by D-penicillamine and phenylacetate, inhibitor, and substrate of PON-1, respectively, leading to speculate on the active site. Finally, nitratase activity and albumin contribute to beneficially convert peroxynitrite (ONOO 2 ) into nonbioactive NO 3 2 . But, in inflammatory conditions, xanthine oxidoreductase is expressed leading to detrimentally reduce O 2 and NO 3 2 into O 2 2 and NO that may interact, reconstituting the ONOO 2 pool. The real consequence of nitratase activity and the physiological significance of nitration/denitration processes remain to be explored. IUBMBIUBMB Life, 60(1): [73][74][75][76][77][78] 2008

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Section: Discussionmentioning

confidence: 99%

First evidence for an LDL‐ and HDL‐associated nitratase activity that denitrates albumin‐bound nitrotyrosine—Physiological consequences

Léger

Torres‐Rasgado²,

Fouret³

et al. 2007

IUBMB Life

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“…For example, the reaction with ozone and nitrogen oxides leads to the formation of reactive oxygen intermediates and nitrated proteins that can influence the interaction of PBAP with the immune system and trigger or exacerbate allergic diseases (Gruijthuijsen et al, 2006;Shiraiwa et al, 2011;Zhang et al, 2011).…”

Section: Pollenmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

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1,114

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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“…Fungal spores' potential to induce allergies in humans has been recently connected to environmental changes such as elevated CO 2 concentration and carbon content of the growth material (Lang-Yona et al, 2013). Pollen was shown to change their nitration state when exposed to urban air pollution, leading to a possible change in their allergenic potency (D 'Amato et al, 2001;Franze et al, 2005;Gruijthuijsen et al, 2006;Shiraiwa et al, 2011a;ReinmuthSelzle et al, 2014).…”

Section: Primary Biological Aerosol Particles (Pbaps)mentioning

confidence: 99%

Particulate matter, air quality and climate: lessons learned and future needs

et al. 2015

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Abstract. The literature on atmospheric particulate matter (PM), or atmospheric aerosol, has increased enormously over the last 2 decades and amounts now to some 1500-2000 papers per year in the refereed literature. This is in part due to the enormous advances in measurement technologies, which have allowed for an increasingly accurate understanding of the chemical composition and of the physical properties of atmospheric particles and of their processes in the atmosphere. The growing scientific interest in atmospheric aerosol particles is due to their high importance for environmental policy. In fact, particulate matter constitutes one of the most challenging problems both for air quality and for climate change policies. In this context, this paper reviews the most recent results within the atmospheric aerosol sciences and the policy needs, which have driven much of the increase in monitoring and mechanistic research over the last 2 decades.The synthesis reveals many new processes and developments in the science underpinning climate-aerosol interactions and effects of PM on human health and the environment. However, while airborne particulate matter is responsible for globally important influences on premature human mortality, we still do not know the relative importance of the different chemical components of PM for these effects. Likewise, the magnitude of the overall effects of PM on climate remains highly uncertain. Despite the uncertainty there are many things that could be done to mitigate local and global problems of atmospheric PM. Recent analyses have shown that reducing black carbon (BC) emissions, using known control measures, would reduce global warming and delay the time when anthropogenic effects on global temperature would exceed 2 • C. Likewise, cost-effective control measures on ammonia, an important agricultural precursor gas for secondary inorganic aerosols (SIA), would reduce regional eutrophication and PM concentrations in large areas of Europe, China and the USA. Thus, there is much that could be done to reduce the effects of atmospheric PM on the climate and the health of the environment and the human population.A prioritized list of actions to mitigate the full range of effects of PM is currently undeliverable due to shortcomings in the knowledge of aerosol science; among the shortcomings, the roles of PM in global climate and the relative roles of Published by Copernicus Publications on behalf of the European Geosciences Union. 8218S. Fuzzi et al.: Particulate matter, air quality and climate different PM precursor sources and their response to climate and land use change over the remaining decades of this century are prominent. In any case, the evidence from this paper strongly advocates for an integrated approach to air quality and climate policies.

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Nitration Enhances the Allergenic Potential of Proteins

Cited by 117 publications

References 49 publications

First evidence for an LDL‐ and HDL‐associated nitratase activity that denitrates albumin‐bound nitrotyrosine—Physiological consequences

First evidence for an LDL‐ and HDL‐associated nitratase activity that denitrates albumin‐bound nitrotyrosine—Physiological consequences

Primary biological aerosol particles in the atmosphere: a review

Particulate matter, air quality and climate: lessons learned and future needs

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