Nitrate Transport System in
            <i>Neurospora crassa</i>

Rh, Schloemen; Rh, Garrett

doi:10.1128/jb.118.1.259-269.1974

Cited by 79 publications

(21 citation statements)

References 32 publications

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“…In their view these low pH values result in a displacement of membrane-bound Ca^+ and thus prevent the uptake of nitrate. Schloemer and Garrett (1974)…”

Section: Discussionmentioning

confidence: 99%

“…The stimulation by NH4+ of the release may possibly be caused by the H+ effect on the membranes (Minotti et al 1969). The decreased uptake rate might be caused by an inhbition by NH4+ [possibly non-competitive as described by Schloemer and Garrett (1974)1. But since the experiments lasted for several hours, an inhibition by assimilation products of NH4+ (Doddema et al 1978) cannot be excluded.…”

Section: Demonstrated Inmentioning

confidence: 99%

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Uptake of Nitrate by Mutants of Arabidopsis thaliana, Disturbed in Uptake or Reduction of Nitrate

Doddema

Telkamp

1979

Physiologia Plantarum

217

113

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The uptake of nitrate by wildtype plants and chlorate‐resistant mutants of Arabidopsis thaliana (L.) Heynh. was studied by intermittent or continuous measurement of the nitrate concentration of the ambient solution. The uptake rate in the wildtype and the nitrate reductase‐less mutant B 25 showed a dual‐phase relation ship with concentration. Each phase showed Michaelis‐Menten kinetics although multiphasic patterns within each phase could not be excluded. A dual‐phase relationship was also found in the uptake mutant B I. Here, however, phase II did not follow Michaelis‐Menten kinetics and uptake rate of nitrate in the phase II concentration range was considerably lower in the B 1 mutant than in the wildtype. It is concluded that the mutation in B I has disturbed phase II of the nitrate uptake, without affecting phase I, which leads to the suggestion that uptake of nitrate in Arabidopsis is mediated by at least two independent uptake mechanisms. The nitrate uptake rate showed an optimum at pH 8, and it was not stimulated by the presence of calcium. Ammonium had different effects on nitrate uptake: a direct effect, when it was present during the uptake of nitrate, resulting in a release of nitrate and a reduced rate of uptake, and an indirect inhibitory effect, possibly caused by assimilation products of ammonium, which is most pronounced after growth on ammonium as the sole nitrogen source or in long‐lasting uptake experiments in the presence of ammonium. Chlorate also showed a multiple effect, an inhibiting one which proved to be competitive and, at very low concentrations of chlorate, a stimulating one. Evidence was obtained that chlorate and nitrate arc taken up by the same carrier.

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“…In their view these low pH values result in a displacement of membrane-bound Ca^+ and thus prevent the uptake of nitrate. Schloemer and Garrett (1974)…”

Section: Discussionmentioning

confidence: 99%

Section: Demonstrated Inmentioning

confidence: 99%

Uptake of Nitrate by Mutants of Arabidopsis thaliana, Disturbed in Uptake or Reduction of Nitrate

Doddema

Telkamp

1979

Physiologia Plantarum

217

113

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“…Increase in uptake rate during the first hours of exposure to NO3" has been reported for both lower (Schloemer and Garrett 1974) and higher (Jackson 1978) plants. For a number of higher plant species, the apparent induction of NO3" uptake depends on the presence of photosynthetically active organs (Jackson et al 1976) or of endosperm (Jackson et al 1973).…”

Section: Nitrate Uptakementioning

confidence: 91%

Time‐course of Nitrate Uptake and Nitrate Reductase Activity in Nitrogen‐depleted Dwarf Bean

Breteler

Cate

Nissen

1979

Physiologia Plantarum

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The rate of nitrate uptake by N‐depleted French dwarf bean (Phaseolus vulgaris L. cv. Witte Krombek) increased steadily during the first 6 h after addition of NO3 ‐After this initial phase the rale remained constant for many hours. Detached root systems showed the same time‐course of uptake as roots of intact plants. In vivo nitrate reductase activity (NRA) was assayed with or without exogenous NO3‐ in the incubation medium and the result ing activities were denoted potential and actual level, respectively. In roots the difference between actual and potential NRA disappeared within 15 min after addition of nitrate, and NRA increased for about 15 h. Both potential and actual NRA were initially very low. In leaves, however, potential NRA was initially very high and was not affected by ambient nitrate (0.1–5 mol m‐3) for about 10 h. Actual and potential leaf NRA became equal after the same period of time. In the course of nitrate nutrition, the two nitrate reductase activities in leaves were differentially inhibited by cycloheximide (3.6 mmol m‐3) and tungstate (1 mol m‐3). We suggest that initial potential NRA reflects the activity of pre‐existing enzyme, whereas actual NRA depends on enzyme assembly during NO3‐ supply. Apparent induction of nitrate uptake and most (85%) of the actual in vivo NRA occurred in the root system during the first 6 h of nitrate utilization by dwarf bean.

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“…Behind the tip, within the mycelial network, ion and nutrient uptake are driven by the activity of the plasma membrane H + -ATPase. Some of the known (and assumed, such as Cl -) H + co-transporters are shown, including those for amino acids 110 , nitrate 111,112 , phosphate 113 , and glucose 114 . SPRAY, a putative regulator of calcium sequestration, 5…”

Section: Figuresmentioning

confidence: 99%

How does a hypha grow? The biophysics of pressurized growth in fungi

2011

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The mechanisms underlying the growth of fungal hyphae are rooted in the physical property of cell pressure. Internal hydrostatic pressure (turgor) is one of the major forces driving the localized expansion at the hyphal tip which causes the characteristic filamentous shape of the hypha. Calcium gradients regulate tip growth, and secretory vesicles that contribute to this process are actively transported to the growing tip by molecular motors that move along cytoskeletal structures. Turgor is controlled by an osmotic mitogen-activated protein kinase cascade that causes de novo synthesis of osmolytes and uptake of ions from the external medium. However, as discussed in this Review, turgor and pressure have additional roles in hyphal growth, such as causing the mass flow of cytoplasm from the basal mycelial network towards the expanding hyphal tips at the colony edge.

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Nitrate Transport System in Neurospora crassa

Cited by 79 publications

References 32 publications

Uptake of Nitrate by Mutants of Arabidopsis thaliana, Disturbed in Uptake or Reduction of Nitrate

Uptake of Nitrate by Mutants of Arabidopsis thaliana, Disturbed in Uptake or Reduction of Nitrate

Time‐course of Nitrate Uptake and Nitrate Reductase Activity in Nitrogen‐depleted Dwarf Bean

How does a hypha grow? The biophysics of pressurized growth in fungi

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