Nitrate supply induces changes in polyamine content and ethylene production in wheat plants grown with ammonium

Garnica, María; Houdusse, Fabrice; Yvin, Jean Claude; García-Mina, José M.

doi:10.1016/j.jplph.2008.06.008

Cited by 33 publications

(26 citation statements)

References 33 publications

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“…This is consistent with data reported in subsequent studies where NO 3 − reduced putrescine content in shoots, but had no effect in roots. 14 As in the case of the amino acids mentioned above, Ser and Ala contents were also increased when NO 3 − was supplied in the nutrient solution (Figs 4 and 5). Ser is a product of photorespiration and its biosynthesis generates NH 4 + .…”

Section: Amino Acid Content Depends On the Form Of N Supplied In The mentioning

confidence: 59%

“…Certain studies suggest that it might be related to changes in the physiological pH, the maintenance of appropriate carboxylate levels in plants 13 or to specific changes in the plant content of certain plant hormones and/or polyamines. 7,14 This action of NO 3 − on the development of plants fed with NH 4 + and/or urea might also be mediated by effects on the root uptake and further assimilation of NH 4 + and urea. In previous studies we have demonstrated that the beneficial effect of NO 3 − seems to be related to an enhancement of NH 4 + and urea root uptake, although their assimilation seemed to be significantly increased in urea-fed plants but not in NH 4 + -fed plants.…”

Section: Nomentioning

confidence: 99%

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Nitrate modifies the assimilation pattern of ammonium and urea in wheat seedlings

Garnica¹,

Houdusse²,

Zamarreño³

et al. 2009

J Sci Food Agric

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These results suggest that the effect of nitrate is compatible with a rapid and transient increase in the activity of glutamine synthetase and urease during the first hour after the onset of treatments. Nevertheless, a possible effect of nitrate reducing ammonium accumulation through the activation of alternative metabolic pathways different from that involving glutamine synthetase cannot be ruled out. Finally, nitrate effects on amino acid concentration indicate that, whereas ammonium assimilation takes place principally in the root, urea and nitrate assimilation occurred in the shoot, under the conditions of the experiment.

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Section: Amino Acid Content Depends On the Form Of N Supplied In The mentioning

confidence: 59%

Section: Nomentioning

confidence: 99%

Nitrate modifies the assimilation pattern of ammonium and urea in wheat seedlings

Garnica¹,

Houdusse²,

Zamarreño³

et al. 2009

J Sci Food Agric

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“…Ethylene inhibited the activities of arginine decarboxylase and SAM decarboxylase thus reducing PAs contents (Bouchereau et al 1999). However, other results showed that PAs inhibited the accumulation of transcript of 1-aminicyclopropane-1-carboxylic acid (precursor of ethylene) synthase (Garnica et al 2009). The correlations observed in our study suggested that in the investigated wheat genotypes, the increase in PA concentration inhibited ethylene synthesis in PEG-treated plants (Table 3).…”

Section: Discussionmentioning

confidence: 95%

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

et al. 2012

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Contents of ethylene, osmoprotectants, levels and forms of polyamines (PAs) and activities of antioxidant enzymes in the leaves and roots were investigated for five wheat cultivar seedlings (differing in drought tolerance) exposed to osmotic stress (-1.5 MPa). Stress was induced by 2-day-long treatment of plants with polyethylene glycol 6000 (PEG) or NaCl added to hydroponic cultures. Nawra, Parabola and Manu cv. (drought tolerant) showed a marked increase in osmoprotectors (proline and soluble carbohydrates, mainly glucose, saccharose and maltose), free PAs (putrescine Put, spermidine Spd and spermine Spm) and Spd-conjugated levels, in both leaves and roots, after PEGtreatments. Radunia and Raweta (drought sensitive) exhibited smaller changes in the content of these substances. The analysis of enzymes involved in proline metabolism revealed the glutamate as a precursor of proline synthesis in PEGinduced stress conditions. The increase in the activity of antioxidative enzymes, especially catalase and peroxidases, was characteristic for tolerant wheat plants, but for sensitive ones, a decrease in superoxide dismutase and an increase in mainly glutathione reductase activities were observed. After NaCl-treatment smaller changes of all biochemical parameters were registered in comparison with PEG-induced stress. Exceptions were the higher values of ethylene content and a significant increase in saccharose, raffinose and maltose levels (only in stress sensitive plants). The proline synthesis pathway was stimulated from both glutamate and ornithine precursors. These results suggest that the accumulation of inorganic ions in NaCl-stressed plants may be involved in protective mechanisms as an additional osmoregultor. Thus, a weaker stressogenic effect as determined as water deficit by leaf relative water content and relative dry weight increase rate and differences in metabolite synthesis in comparison with PEG stress was observed. Proline seems to be the most important osmo-protector in osmotic stress initiated by both PEG and NaCl. The synthesis of sugars and PAs may be stimulated in a stronger stress conditions (PEG).

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“…Under the mixture of nitrogen form, plants displayed remarkable variations in terms of nitrate uptake, assimilation and ammonium metabolism (Raab and Terry 1995;Matraszek 2008;Tabatabaei et al 2008). Garnica et al (2009) proposed that this effect was tightly correlated with endogenous changes of polyamine and ethylene production. Other studies suggested that the synergistic effect might be related to alterations in solution acidification induced by NH 4 ?…”

Section: Introductionmentioning

confidence: 99%

Physiological responses of Catharanthus roseus to different nitrogen forms

Guo

Tang

2011

Acta Physiol Plant

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The responses of carbon and nitrogen metabolisms in the medical plant Catharanthus roseus to the nitrogen solutions (N1, N2 and N3) containing different ratio of nitrate to ammonium (1:0, N1; 1:1, N2; 1:3, N3) were investigated here. The plants in N3 nitrogen solution were strongly inhibited in photosynthetic gas exchange and carbohydrate accumulation, reflecting the toxicity symptom of excess ammonium continuously accumulated in plants. The treatment with N2 nitrogen solution, however, displayed an obviously synergistic effect on plant growth and metabolisms in contrast to nitrate as the sole source. The short-term (7 days) exposure of plants to N2 nitrogen solution resulted in an increased shoot/root ratio, leaf mass ratio, and Pn, as well as the elevated levels of sucrose, glutamate, aspartate, proline and threonine. The plants in N2 nitrogen solution accumulated twofold catharanthine and vinblastine than did the plants in N1 or N3 nitrogen solution after the long-term incubation. Internal nitrate had an increased accumulation in the plants in N2 nitrogen solution compared to the counterparts. The supply of N2 or N3 nitrogen solution to plants for 7 days induced an over tenfold increase of ammonium in leaves as compared to the case using N1 nitrogen solution. The increased ammonium ion promoted the activities of NADH-dependent glutamate dehydrogenase (NADH-GDH) both in the leaf and root of plants. Under the ammonium-containing solution (N2 and N3 nitrogen solutions), there was a significantly increased activity for glutamine synthase (GS) in the root during experiment and for nitrate reductase (NR) in the leaf and root only after 21 days of treatment. The performed correlation analysis revealed a negative relation between soluble sugars and internal ammonium, whereas a positive correlation of alkaloid production with glutamate and aspartate.

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Nitrate supply induces changes in polyamine content and ethylene production in wheat plants grown with ammonium

Cited by 33 publications

References 33 publications

Nitrate modifies the assimilation pattern of ammonium and urea in wheat seedlings

Nitrate modifies the assimilation pattern of ammonium and urea in wheat seedlings

Relationships between polyamines, ethylene, osmoprotectants and antioxidant enzymes activities in wheat seedlings after short-term PEG- and NaCl-induced stresses

Physiological responses of Catharanthus roseus to different nitrogen forms

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