Nitrate signaling and use efficiency in crops

Gao, Yangyang; Qi, Shengdong

doi:10.1016/j.xplc.2022.100353

Cited by 19 publications

(6 citation statements)

References 117 publications

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“…Previous researches on improving NUE are mainly focused on nitrate transport and assimilationrelated genes (Fang et al, 2013;Fan et al, 2014;Chen et al, 2016;Gao et al, 2019). Recently, several nitrate regulatory genes have been reported to play momentous roles in crop NUE (Gao et al, 2022a). NRT1.1 has been characterized as the first nitrate sensor to regulate nitrate signaling in Arabidopsis (Ho et al, 2009;Wang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis eIF4E1 regulates NRT1.1‐mediated nitrate signaling at both translational and transcriptional levels

Duan

et al. 2023

New Phytologist

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Summary Identifying new nitrate regulatory genes and illustrating their mechanisms in modulating nitrate signaling are of great significance for achieving the high yield and nitrogen use efficiency (NUE) of crops. Here, we screened a mutant with defects in nitrate response and mapped the mutation to the gene eIF4E1 in Arabidopsis. Our results showed that eIF4E1 regulated nitrate signaling and metabolism. Ribo‐seq and polysome profiling analysis revealed that eIF4E1 modulated the amount of some nitrogen (N)‐related mRNAs being translated, especially the mRNA of NRT1.1 was reduced in the eif4e1 mutant. RNA‐Seq results enriched some N‐related genes, supporting that eIF4E1 is involved in nitrate regulation. The genetic analysis indicated that eIF4E1 worked upstream of NRT1.1 in nitrate signaling. In addition, an eIF4E1‐interacting protein GEMIN2 was identified and found to be involved in nitrate signaling. Further investigation showed that overexpression of eIF4E1 promoted plant growth and enhanced yield and NUE. These results demonstrate that eIF4E1 regulates nitrate signaling by modulating NRT1.1 at both translational and transcriptional levels, laying the foundation for future research on the regulation of mineral nutrition at the translational level.

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Section: Discussionmentioning

confidence: 99%

The Arabidopsis eIF4E1 regulates NRT1.1‐mediated nitrate signaling at both translational and transcriptional levels

Duan

et al. 2023

New Phytologist

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“…Nitrate serves as a principal N source from soils for plant growth and also as an intriguing signal molecule that regulates many biological processes, including gene expression, root system architecture (Vidal et al, 2020), leaf development (Rahayu et al, 2005), seed dormancy (Alboresi et al, 2005), and flowering time (Castro et al, 2011). To date, the uptake and internal translocation as well as sensing of NO − 3 have been mostly attributed to the molecular action of the NRT1 and NRT2 family of proteins in planta (Carillo and Rouphael, 2022;Gao et al, 2022). Despite the elaborate functional characterization of some NRT1s and NRT2s in certain plant species, including Arabidopsis, rice, and soya beans (Vidal et al, 2020;Carillo and Rouphael, 2022), no individual molecular component responsible for NO − 3 movement in cultivated tobacco has been described.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and physiological functional analysis of NtNRT1.1B that mediated nitrate long-distance transport and improved plant growth when overexpressed in tobacco

Xiang²,

Huang³

et al. 2023

Front. Plant Sci.

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Although recent physiological studies demonstrate that flue-cured tobacco preferentially utilizes nitrate (NO3−) or ammonium nitrate (NH4NO3), and possesses both high- and low-affinity uptake systems for NO3−, little is known about the molecular component(s) responsible for acquisition and translocation in this crop. Here we provide experimental data showing that NtNRT1.1B with a 1,785-bp coding sequence exhibited a function in mediating NO3− transport associated with tobacco growth on NO3− nutrition. Heterologous expression of NtNRT1.1B in the NO3− uptake-defective yeast Hp△ynt1 enabled a growth recovery of the mutant on 0.5 mM NO3−, suggesting a possible molecular function of NtNRT1.1B in the import of NO3− into cells. Transient expression of NtNRT1.1B::green fluorescent protein (GFP) in tobacco leaf cells revealed that NtNRT1.1B targeted mainly the plasma membrane, indicating the possibility of NO3− permeation across cell membranes via NtNRT1.1B. Furthermore, promoter activity assays using a GFP marker clearly indicated that NtNRT1.1B transcription in roots may be down-regulated by N starvation and induced by N resupply, including NO3−, after 3 days’ N depletion. Significantly, constitutive overexpression of NtNRT1.1B could remarkably enhance tobacco growth by showing a higher accumulation of biomass and total N, NO3−, and even NH4+ in plants supplied with NO3−; this NtNRT1.1B-facilitated N acquisition/accumulation could be strengthened by short-term 15N-NO3− root influx assays, which showed 15%–20% higher NO3− deposition in NtNRT1.1B-overexpressors as well as a high affinity of NtNRT1.1B for NO3− at a Km of around 30–45 µM. Together with the detection of NtNRT1.1B promoter activity in the root stele and shoot–stem vascular tissues, and higher NO3− in both xylem exudate and the apoplastic washing fluid of NtNRT1.1B-transgenic lines, NtNRT1.1B could be considered as a valuable molecular breeding target aiming at improving crop N-use efficiency by manipulating the absorption and long-distance distribution/transport of nitrate, thus adding a new functional homolog as a nitrate permease to the plant NRT1 family.

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“…The Dof transcription factors are known to participate in various biological processes such as photoresponse, photoperiod regulation, owering, seed development, germination, plant hormone signaling, defense response, and nitrogen metabolism [32] . Notably, ZmDof1, identi ed in both Arabidopsis and rice, has been found to enhance nitrogen assimilation and promote plant growth, particularly under conditions of nitrogen de ciency [33] . Additionally, apart from the transcription factors speci cally linked to nitrogen metabolism, the discovery of ERF transcription factors, closely related to seed development [34] , has provided further insights into the genetic landscape of millet growth and development.…”

Section: Chromosomal Location Of Degsmentioning

confidence: 99%

Transcriptomics-based Mining and Analysis of Low Nitrogen Tolerance Related Genes in Foxtail Millet

Qin,

Fu,

Zhu

et al. 2024

Preprint

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Background: Nitrogen (N) is a nutrient element crucial for the growth and development of crops. A better understanding of the effects of low-N stress on millet (Setaria italica L.) production, a model crop for studying low-N tolerance, and the underlying molecular mechanisms responsible for low-N stress responses is vital to improvement of agricultural production. Results: In this study, we used RILs as materials, its parental lines ‘Yugu 28,’ a low N-tolerant millet variety, and ‘Qiye Huang,’ a low N-sensitive variety. A transcriptomic study using leaves collected twenty-one days after normal and low-N treatment, the N concentration was 2.5 and 0.5 mmol·L-1, respectively. In total, 894 differentially expressed genes (DEGs) were identified between normal and low-N treatment. Further analysis revealed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to amino acid, carbon, N, and lipid metabolism and secondary metabolite biosynthesis. Co-regulation analysis suggested that 15 DEGs contributed to the formation of a regulatory network involving multiple biological processes and pathways including nitrogen or amino acid biosynthesis, uptake, metabolism and utilization. RNA-seq analysis also showed that some nitrogen metabolism-related DEGs were highly expressed in low-N stress compared to normal N conditions. The changes in expression of low N-responsive genes determined by RNA sequencing were confirmed by reverse-transcription PCR for nine genes. Conclusions:The transcriptome is beneficial for identified the candidate genes of millet in response to low nitrogen stress, SiGS1 (LOC101764285) and SiNPR2 ((LOC101764285)) were identified as potential candidate genes for improving low nitrogen stress tolerance. These results are a first step to understanding the molecular mechanisms of low-N tolerance in foxtail millet and lay a foundation for its genetic improvement.

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Nitrate signaling and use efficiency in crops

Cited by 19 publications

References 117 publications

The Arabidopsis eIF4E1 regulates NRT1.1‐mediated nitrate signaling at both translational and transcriptional levels

The Arabidopsis eIF4E1 regulates NRT1.1‐mediated nitrate signaling at both translational and transcriptional levels

Molecular identification and physiological functional analysis of NtNRT1.1B that mediated nitrate long-distance transport and improved plant growth when overexpressed in tobacco

Transcriptomics-based Mining and Analysis of Low Nitrogen Tolerance Related Genes in Foxtail Millet

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