29In this study, two anoxic-oxic-MBR systems (conventional and biofilm) were operated in 30 parallel under complete SRT to compare system performance and microbial community 31 composition. Moreover, with the microbial communities, comparisons were made between adhesive 32 stage and suspended stage. High average removal of COD, NH 4 + -N and TN was achieved in both 33 systems. However, TP removal efficiency was remarkably higher in BF-A/O-MBR when compared 34 with C-A/O-MBR. TP mass balance analysis suggested that under complete SRT, sponges play a 35 key role in both phosphorus release and accumulation. The qPCR analysis showed that sponge 36 biomass could maintain higher abundance of total bacteria than suspended sludge. Meanwhile,
37AOB and denitrifiers were enriched in the suspended sludge rather than the sponge biomass.
38Results of pyrosequencing reveal that the compacted sponge in BF-A/O-MBR could promote the 39 growth of bacteria involved in nutrient removal and reduce the filamentous and bacterial related to 40 membrane fouling in the suspended sludge. 41 43 44 3 53processes that can enhance the efficiency of biological nutrient removal to safe levels. These 54 processes are referred to as biological nutrient removal (BNR).
55Since autotrophic nitrifying bacteria, namely phosphorus accumulating organisms (PAOs), and 56 heterotrophic bacteria compete with each other for habitat and growth under the same operational 57 conditions, the BNR processes normally become unpromising with regard to simultaneously high 58 organic carbon and nutrient removal [1]. Therefore, it has become extremely important to develop 59 reliable technologies that can simultaneously treat organic carbon and nutrients in municipal 60 wastewater. Several research studies have attempted to combine the advantages of attracting growth 61 biofilm and the membrane bioreactor (MBR) process in order to achieve some of the boundaries of 62 traditional MBR that have been based on the activated sludge process. The application of attracting 63 biofilm in MBR could be achieved by the addition of media (e.g., biofilm carriers) in moving or 64 fixed bed configurations, or by the addition of aerated membranes in the bioreactor as a form of 65 support (i.e., substratum) for biofilm growth. 66 The use of media in the aerobic MBR, called biofilm MBR, could be a better alternative to 67 conventional aerobic MBR which may increase the treatment performance by high biomass 68 concentrations and reduce membrane fouling [2]. Additionally, total nitrogen removal is reported to 69 be higher in the biofilm aerobic MBR systems when compared to conventional aerobic MBR 70 systems according to the authors of several published studies [2,3]. Higher total nitrogen (TN) 71 removal rates have been mostly attributed to simultaneous nitrification/denitrification (SND) that 72 takes place in deeper layers of the biofilm component where anoxic conditions have occurred. The 73sponge carriers seem to provide good SND conditions since they provide anoxic conditions insid...