Nitrate Reductase Activity and Nitrate Content under Two Forms and Three Levels of Nitrogen Nutrition in Lolium perenne L.

Bergareche, Carmen; Simón, Esther

doi:10.1016/s0176-1617(88)80178-0

Cited by 9 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present experiment, nitrate concentrations in leaves and stems did not reach the potentially toxic levels of 0.34% NO~--N reported by Wright and Davison (1964) in other Gramineae and they were below 0.22% which is generally considered as a safe level (Eck et al, 1981). Nitrate-N concentrations obtained in bermudagrass were much lower than the ones we earlier found in perennial ryegrass growing in the same soil (Bergareche and Simon, 1988). Decker et at., (1971) also found that warm-season grasses like Cynodon with a rhizomatous habit have a tendency to accumulate nitrate only to modest levels.…”

Section: Discussioncontrasting

confidence: 72%

See 1 more Smart Citation

Nitrate and ammonium accumulation in bermudagrass in relation to nitrogen fertilization and season

Bergareche

Simón

1989

Plant Soil

View full text Add to dashboard Cite

Seasonal changes in nitrate and ammonium concentrations were studied in Cynodon dactylon (L.) Pers.plants grown for one year in the field in a Mediterranean area. Plants cultivated in a sandy loam soil were fertilized with nitrate-N or ammonium-N at two application rates (250 and 1000 kg N ha-~ year ~) and compared to controls with no added N. Plots were harvested every three weeks from May to November. Shoots were separated into leaves and stems and analyses carried out in both fractions.Nitrogen applications generally led to elevated nitrate concentrations both in leaves and stems at all sampling dates but had little influence on the ammonium concentrations of the tissues.Higher nitrate and ammonium concentrations were found in stems than in leaves, although no levels higher than 0.22% NO~-N and 0.10% NH~--N were detected in either fraction. Nitrate tended to accumulate mostly in autumn and spring whereas low accumulations were found in summer. Ammonium showed both in leaves and stems a progressive but limited accumulation throughout the period with a peak in October, followed by a strong decrease in November.

show abstract

Section: Discussioncontrasting

confidence: 72%

“…Nitrate concentrations were almost 50% lower in midsummer than in autumn. Several environmental factors such as short day length, low light intensity, low temperatures and high levels of nitrate in the experimental soil (Bergareche and Simon, 1988) determined the nitrate accumulation during autumn.…”

Section: Discussionmentioning

confidence: 99%

Nitrate and ammonium accumulation in bermudagrass in relation to nitrogen fertilization and season

Bergareche

Simón

1989

Plant Soil

View full text Add to dashboard Cite

show abstract

“…Our results are similar to those of Sun et al (2009) and they reported that with increasing the N application rates, the activities of GS, GA, GOGAT, and NR in rice leaves increased at various growth stages. The activity of NR was found to increase with increasing NOj~-N and NH^''"-N concentrations (Bergareche and Simon, 1988). Similarly, Pabby et al (2001) and Finnemann and Schjoerring (1998) also reported that the activities of GS and GOGAT in the rice leaves increased with increasing concentrations of NO^^-N at 14 d after the treatment.…”

Section: Effects Of Nitrogen Sources and Rates On The Activities Of Gmentioning

confidence: 68%

“…Ntamatungiro et al (1999) fotind that increasing the number of topdressings from the middle to the late stage of rice growth could increase N uptake and grain yield. In addition, many studies have shown GS, GOGAT, and NR to be the main enzymes involved in the N metabolism of plants (Zhang et al, 2000;Sun et al, 2012).The activities of GOGAT and NR were increased in rice leaves as a result of increased concentrations of NOj^-N and NH^"'"-N in soil water solution (Bergareche and Simon, 1988). Some reports have asserted that certain CRUs enhance N uptake and increased rice grain yield through sustainable nutrient release from fertilizers (Kaneta et al, 1994;Shoji and Kanno, 1994).…”

mentioning

confidence: 99%

Controlled Release Urea Improved Nitrogen Use Efficiency, Activities of Leaf Enzymes, and Rice Yield

Yang

Zhang

et al. 2012

Soil Science Soc of Amer J

143

View full text Add to dashboard Cite

The effects of a controlled release urea (CRU) on concentration of N in root zones, activities of N metabolism enzymes in leaves, and N use efficiency of rice (Oryza sativa L.) grown on a silt loam soil in lysimeters were investigated. The entire amount (100–300 kg N ha−1) of the CRU was incorporated into a plug for growing the rice seedlings while the regular urea was split into three applications (the standard practice). Results indicated that the N release of CRU closely matched the N requirements of the rice, and the commingling of the CRU with the rice seeds increased N concentration within the root zone and enhanced the activities of glutamine synthetase (GS), glutamine 2‐oxoglutarate amidotransferase (GOGAT) and nitrate reductase (NR) in leaves. At the same N rate, the apparent N use efficiency of the CRU applied at 300 kg N ha−1 was 27.6 and 22.9% higher than that of conventional urea treatment in 2007 and 2008, respectively. Although the CRU at 200 kg N ha−1 supplied one‐third less N than the urea at 300 kg N ha−1, the CRU produced 3 to 5.9% more grain than the urea. Placing the CRU with rice seeds without additional fertilizer application during the entire growing season significantly increased N availability in soils and improved rice growth and led to use of less N fertilizers for greater rice grain yield.

show abstract

“…NR assays were conducted in vivo using the method by BarAkiva et al (1970), as modified by Blondel and Blanc (1975) who took into account different factors which may interfere with nitrite exodiffusion, such as wetting of piant material, size of leaf fragments, saline concentration of the incubation solution, and kind of surfactant used, as described elsewhere (Bergareche and Simon 1988). For the analyses cotyledons were incitbated with 4 ml medium containing; 0.05 rrunol Tris buffer, HCl 1.5 M pH 7.4, Tween 80 0.02% (w/w), and 0.5 mmol KNO,.…”

Section: Enzyme Analysismentioning

confidence: 99%

Nitrate reductase in cotyledons of cucumber seedlings as affected by nitrate, phytochrome and calcium

Bergareche

Ayuso

Masgrau

et al. 1994

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

1994. Nitrate reductase in cotyledons of cucumber seedlings as affected by nitrate, phytochrome and calcium. -Physiol. Plant. 91: 257-262.Regulation by the active form of phytochrome (PRJ) and the effect of Ca-* was examined with nitrate re.ducta.se (NR) in etiolated cucumber (Cucumis sativus cv. Beilpuig). Nitrate reductase activity (NRA) was studied in excised coty'iedons of cucumber seedlings grown io distilled water and in darkness for seven days at 24 ± 0.5°C. All experiments were performed in the dark and a dim green safeiight was used during analyses, lo etiolated cucumber cotyledons NRA was induced by nitrate and a brief irradiation (15 min) with red light (R) resulted in 62% increase in NRA. This effect was nullified when R was followed immediately by a brief (5 min) far-red light (FR). NRA also showed a semidian (12 h) rhythmicity. Both PpR, and nitrate effects were age depefideot. Calcium seemed to be involved since the phytochrome effect was only obseri'ed when calcium was supplied in the external solution. The effect of R on NRA depended on the period of calcium nitrate incubation. An extensal supply of calcium ionophore mimicked the effect of R and, if supplied to R-irradiated cotyledons, produced a higher NR level than that caused by R alone. This suggested that intracellolar free calciutn was involved.

show abstract

Nitrate Reductase Activity and Nitrate Content under Two Forms and Three Levels of Nitrogen Nutrition in Lolium perenne L.

Cited by 9 publications

References 22 publications

Nitrate and ammonium accumulation in bermudagrass in relation to nitrogen fertilization and season

Nitrate and ammonium accumulation in bermudagrass in relation to nitrogen fertilization and season

Controlled Release Urea Improved Nitrogen Use Efficiency, Activities of Leaf Enzymes, and Rice Yield

Nitrate reductase in cotyledons of cucumber seedlings as affected by nitrate, phytochrome and calcium

Contact Info

Product

Resources

About