Nitrate or ammonium nutrition in french bean

Chaillou, Sylvain; Morot‐Gaudry, Jean‐François; Lesaint, C.; Salsac, L.; Jolivet, E.

doi:10.1007/978-94-009-4356-8_44

Cited by 14 publications

(15 citation statements)

References 6 publications

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“…Leaf necrosis is characteristic in case of white lupin plants using only the ammonium form, while chlorosis only the nitrate form (Ciesiołka et al 2003). In the research of Chaillou et al (1986), bean plants absorbed nitrate form faster and in greater amounts than the ammonium form, which is also confirmed by the author's own research results on the dynamics of both forms.…”

Section: Resultssupporting

confidence: 74%

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EFFECT OF Nmin CONTENT ON NODULATION IN YELLOW LUPIN (Lupinus luteus L.) IN THE PRESENCE OF Bradyrhizobium lupini AND GENISTEIN

Prusiński¹

2014

JCEA

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d.w. -very high content. Seeds of yellow lupin were inoculated with agar inoculum containing Bradyrhizobium lupini with or without the addition of genistein. Along with the plant growth, gradual decrease in the mean content of Nmin was observed, especially in the nitrogenous form until the stage of full bloom of lupin plants. No significant effect of the Nmin content was found on the initiation of the nodulation process, number and dry weight of nodules, independently of seed inoculation with Bradyrhizobium lupini and application of genistein. Average or higher content of Nmin in the medium was the reason of the lack of survival in symbiotic bacteria Bradyrhizobium lupini capable of nodulation in the medium after lupin harvest. Only in the case of low and very low Nmin content in the medium, the addition of genistein to bacterial inoculum did affect the increase in the survival of symbiotic bacteria after lupin harvest. sm -bardzo wysoka I czynnik B -sposób przygotowania nasion do siewu: b1-kontrola -nasiona zaprawiane, nie szczepione, b2 -nasiona zaprawiane i szczepione agarową szczepionką zawierającą Bradyrhizobium lupini i b3 -nasiona zaprawiane i szczepione agarową szczepionką zawierającą Bradyrhizobium lupini z dodatkiem genisteiny (4',5,7-trihydroksyizoflawon). Każdą kombinację zakładano w 8 powtórzeniach (120 wazonów Kocha o średnicy 22,5 cm i wysokości 25 cm z pełnym dnem). Zawartość Nmin w kombinacjach a2 -a5 uzyskano poprzez dodanie stosowej ilości 34% saletry amonowej zawierającej po 50% N-NO 3 i N-NH 4 . Do każdego wazonu wysiewano po 9 nasion, a po wschodach do dalszych analiz pozostawiano po 5 roślin. Po 7 (c1), 14 (c2), 21 (c3), 28 (c4) i 35 dniach (c5) od początku wschodów, a następnie w fazie pełni kwitnienia (c6) oraz w dojrzałości fizjologicznej (c7) i pełnej nasion (c8) likwidowano kolejno po 15 wazonów. Liczbę brodawek i ich suchą masę (po wysuszeniu w temperaturze 60 o C przez 24 godziny) oznaczono w fazie pełni kwitnienia i w dojrzałości fizjologicznej nasion. Po zbiorze nasion próby podłoża poddawano ocenie zawartości Nmin metodą spektrofotometrycznego pomiaru stężenia jonów azotanowych i amonowych w wyciągu glebowym 1% (m/m) roztworu siarczanu(VI) potasu. Występowanie oraz liczebność bakterii symbiotycznych w podłożu po zbiorze każdego wazonu określono metodą biotestu NPL (Najbardziej Prawdopodobnej Liczebności) po zbiorze nasion. Wraz z wegetacją roślin łubinu żółtego obserwowano stopniowe zmniejszanie się średniej zawartości obu form Nmin w podłożu do fazy pełni kwitnienia, po czym następował stopniowy i umiarkowany wzrost ich zawartości do końca okresu wegetacji. Pierwsze brodawki zaobserwowano 14 dnia po wschodach na wszystkich obiektach czynnika A, kiedy średnia zawartość Nmin w podłożu wynosiła aż 17,1 mg . kg -1 , tj. 76,9 kg N . ha -1 Key words

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Section: Resultssupporting

confidence: 74%

“…in bean, stronger than in the nitrate form (Chaillou et al 1986), whereas according to Guo et al (1992), any mineral form of nitrogen, especially the ammonium form, contributes to the reduction in the activity of nitrogenase, in the process of nodulation and dry weight of nodules in faba bean and white lupin.…”

Section: Resultsmentioning

confidence: 99%

EFFECT OF Nmin CONTENT ON NODULATION IN YELLOW LUPIN (Lupinus luteus L.) IN THE PRESENCE OF Bradyrhizobium lupini AND GENISTEIN

Prusiński¹

2014

JCEA

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“…All leaf samples were from plants which were watered with a nutrient solution containing 1 mM Ca (NO3)219]. Total root RNA was prepared from roots of plants grown for 10 days in perlite and watered with a nutrient solution lacking a nitrogen source [9]. Total nodule RNA was prepared from nodules harvested 13 days after inoculation of plants grown as described by Chen and Culli-…”

Section: Plant Materialsmentioning

confidence: 99%

Regulation of glutamine synthetase genes in leaves of Phaseolus vulgaris

Cock

Watson

et al. 1991

Plant Mol Biol

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Glutamine synthetase (GS) activity increased over three-fold in developing primary leaves of Phaseolus vulgaris L. This increase was shown to be the result of differential expression of three members of the GS gene family: gln-alpha and gln-beta, which encode cytosolic GS polypeptides, and gln-delta, which encodes the chloroplast-located GS. The gln-delta gene was the most highly expressed GS gene and was regulated in a complex manner with two different transcripts accumulating differentially during leaf development. This gene was expressed weakly in the dark and was induced strongly by light; this induction was shown not to be an indirect effect of photorespiration. In the long term, gln-delta showed increased expression in photorespiring compared with non-photorespiring leaves. However, in the short term, there was no induction of gln-delta following transfer of plants to photorespiratory conditions. These results suggest that regulation of gln-delta by photorespiration was the result of indirect, long-term effects on cellular metabolism. In general, in all these experiments, analysis of cytosolic versus chloroplastic GS polypeptides and of the GS isoenzyme profiles showed the same pattern of changes in abundance as that observed for the mRNAs suggesting that regulation of GS gene expression occurred primarily at the mRNA level. However, it was noteworthy that the delta isoenzyme remained at a high abundance in older leaves, grown in both light and dark, despite a decrease in abundance of gln-delta mRNA.

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“…s-1 at the level of the seed-trays; Omega, Stanmore, UK) and the remainder were left in the dark. Moreover, half of each of these two sets of seedlings were cultured in a nutrient solution with or without a 2 mM nitrate supplement (Chaillou et al 1986). Cotyledon samples were harvested from dry and overnight-imbibed seeds and from germinating seedlings at 2, 4, 6, 8, 10 and 12 d after sowing.…”

Section: Methodsmentioning

confidence: 99%

Expression of glutamine-synthetase genes in cotyledons of germinating Phaseolus vulgaris L.

Swarup

Bennett

Cullimore

1991

Planta

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In the legume Phaseolus vulgaris L., glutamine synthetase (GS; EC.6.3.1.2.) is encoded by four actively transcribed genes, gln-α, gln-β, gln-γ and gln-δ. We have studied the expression of these genes in cotyledons during seed germination and have studied the effect of light and nitrate on this process. An RNase-protection method, used to detect the abundances of GS mRNAs, revealed that the four GS genes are differentially expressed in the germinating cotyledons. The gln-α. mRNA was present in dry seeds and was the most abundant GS mRNA during early stages of germination. The gln-β and gln-γ mRNAs were first detectable 2 d after sowing and their abundances differed in light- and dark-grown cotyledons at later stages of germination. The gln-δ mRNA (which encodes the plastid-located GS) was detectable only in light-grown cotyledons, at a low abundance. A nitrate supply of 2 mM had only a minor effect on the expression of the GS genes. Western immunodetection and ion-exchange high-performance liquid chromatography demonstrated that the α polypeptide and isoenzyme were present in extracts of dry seeds and represented the major GS products at 2 d and 4 d. Both the β and γ polypeptides appeared at the 2-d stage. The role of differential GS gene expression in controlling cotyledonary GS activity is discussed.

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Nitrate or ammonium nutrition in french bean

Cited by 14 publications

References 6 publications

EFFECT OF Nmin CONTENT ON NODULATION IN YELLOW LUPIN (Lupinus luteus L.) IN THE PRESENCE OF Bradyrhizobium lupini AND GENISTEIN

EFFECT OF Nmin CONTENT ON NODULATION IN YELLOW LUPIN (Lupinus luteus L.) IN THE PRESENCE OF Bradyrhizobium lupini AND GENISTEIN

Regulation of glutamine synthetase genes in leaves of Phaseolus vulgaris

Expression of glutamine-synthetase genes in cotyledons of germinating Phaseolus vulgaris L.

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