Nitrate induction in spruce: an approach using compartmental analysis

Kronzucker, Herbert J.; Glass, Anthony D. M.; Siddiqi, M. Yaeesh

doi:10.1007/bf00197332

Cited by 27 publications

(49 citation statements)

References 40 publications

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“…, 1989). In conifers, NO 3 − induction has been studied in detail in nonmycorrhizal Picea glauca (Kronzucker et al. , 1995a,b) and Pinus contorta (Min et al.…”

Section: Discussionmentioning

confidence: 99%

“…This is probably due to the fact that, at 0.05 mM NO 3 − , the unidirectional efflux is low. Indeed, it was shown in spruce that the ratio between the net flux and the influx measured in 0.1 mM NO 3 − was high, although it varied with the duration of induction with values ranging from 0.73 (day 0) to 0.94 (day 6) (Kronzucker et al. , 1995a).…”

Section: Discussionmentioning

confidence: 99%

“…, 1989). In contrast to herbaceous species, only a few detailed studies have been carried out on tree species, especially coniferous ones (Kronzucker et al. , 1995a,b; Min et al.…”

Section: Introductionmentioning

confidence: 99%

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Differential NO₃⁻ dependent patterns of NO₃⁻ uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

Gobert

Plassard

2002

New Phytologist

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Summary• A different NO 3 -dependent pattern of NO 3 -uptake at low (0 -0.1 mM) is shown in Pinus pinaster and in the ectomycorrhizal fungus Rhizopogon roseolus . In ectomycorrhizal symbiosis, the fungal pattern is pre-eminent.• Net NO 3 -uptake rates were deduced in plant and fungus from solution depletion measurements. Net NO 3 -fluxes were estimated at the surface of mycorrhizal and nonmycorrhizal short roots, using NO 3 -selective microelectrodes.• In NO 3 -starved seedlings, maximum NO 3 -uptake rates were reached after 3 d of incubation in 0.05 mM NO 3 -. In R. roseolus mycelia, NO 3 -uptake rates did not change after withdrawing NO 3 -for up to 7 d, or after adding NO 3 -for 3 d. Net NO 3 -fluxes in nonmycorrhizal short roots were increased twofold by a 3-d exposure to NO 3 -whereas in ectomycorrhiza they were similar irrespective of the NO 3 -pretreatment, but always higher than the fluxes measured in nonmycorrhizal roots.• Ectomycorrhiza have a greater capacity to use NO 3 -than nonmycorrhizal short roots, whatever the NO 3 -concentration in the solution. This may give mycorrhizal plants a greater ability to use fluctuating concentrations of NO 3 -in the soil solution.

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“…, 1989). In conifers, NO 3 − induction has been studied in detail in nonmycorrhizal Picea glauca (Kronzucker et al. , 1995a,b) and Pinus contorta (Min et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Differential NO₃⁻ dependent patterns of NO₃⁻ uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

Gobert

Plassard

2002

New Phytologist

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“…Compartmental analysis by tracer efflux was used to estimate subcellular fluxes and compartmental concentrations (described here in brief; for details, see Lee & Clarkson 1986;Siddiqi, Glass & Ruth 1991;Kronzucker, Siddiqi & Glass 1995;Britto et al . 2001;Kronzucker, Szczerba & Britto 2003; (Hamilton, Ontario, Canada).…”

Section: Flux and Compartmentation Experimentsmentioning

confidence: 99%

The cytosolic Na⁺ : K⁺ ratio does not explain salinity‐induced growth impairment in barley: a dual‐tracer study using ⁴²K⁺ and ²⁴Na⁺

Kronzucker

Szczerba

Moazami-Goudarzi

et al. 2006

Plant Cell & Environment

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“…

Short-term studies with 13 NH 4 ϩ have provided estimates of NH 4 ϩ influx, efflux, and cytoplasmic concentration in spruce (Kronzuker et al, 1995a(Kronzuker et al, , 1995b. As the NH 4 ϩ concentration in the external solution increased from 10 to 1500 m, cytosolic NH 4 ϩ levels increased from 2 to 33 mm and efflux increased from 10% to 35% of influx.

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confidence: 99%

Source and Magnitude of Ammonium Generation in Maize Roots

Volk

Jackson

1998

Plant Physiology

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Short-term studies with 13 NH 4 ϩ have provided estimates of NH 4 ϩ influx, efflux, and cytoplasmic concentration in spruce (Kronzuker et al., 1995a(Kronzuker et al., , 1995b. As the NH 4 ϩ concentration in the external solution increased from 10 to 1500 m, cytosolic NH 4 ϩ levels increased from 2 to 33 mm and efflux increased from 10% to 35% of influx. Similar rates were reported for rice: as external NH 4 ϩ levels increased from 2 to 1000 m, cytosolic NH 4 ϩ levels increased from 3 to 38 mm and efflux rose from 11% to 29% of influx (Wang et al., 1993 ϩ generation in maize roots was estimated to be 50% faster than concurrent NH 4 ϩ uptake (Jackson, et al., 1993). The potential for substantial generation, recycling, and efflux of endogenous NH 4 ϩ in roots is thus indicated.A crucial question raised by these observations is whether protein turnover is the source of endogenous NH 4 ϩ generation, or if recycling of intermediates of the NH 4 ϩ assimilation pathway, such as Gln, is the source. To address this question, maize (Zea mays) seedlings that had been grown on 14 NH 4 ϩ were exposed to highly labeled 15 NH 4 ϩ for 3 d. It was hypothesized that if protein turnover is the source of NH 4 ϩ and if part of this NH 4 ϩ is subject to efflux and translocation to the shoot, a decline in endogenous 14 N protein in the root should occur as new protein is synthesized from the entering 15 NH 4 ϩ . The fact that 15 NH 4 ϩ was applied during six diurnal periods also permitted us to (a) directly measure of the diurnal pattern of NH 4 ϩ fluxes into and out of roots, (b) compare 15 NH 4 ϩ uptake and assimilation by roots during successive light and dark periods, and (c) determine the relationship of the latter processes to carbohydrate levels in shoots and roots. MATERIALS AND METHODS Plant CultureMaize (Zea mays L. cv Pioneer 3320) caryopses were germinated at 30°C in contact with 0.1 mm CaSO 4 . After 30 h, uniform seedlings were selected and their seminal roots excised. Cultures of eight seedlings each were transplanted into 160 L of basal nutrient solution, pH 6.0, containing 0.125 mm (NH 4 ) 2 SO 4 , 1.25 mm K 2 SO 4 , 0.25 mm Ca(H 2 PO 4 ) 2 , 1 mm CaSO 4 , 46 m B, 9 m Mn, 0.8 m Zn, 0.3 m Cu, 0.1 m Mo, and 54 m Fe as ferric diethylenetriamine pentaacetate. The solution was aerated with compressed air that had been washed with H 2 SO 4 and water to remove ambient NH 4 ϩ . A combination of sodium vapor and metal halide lamps provided 1140 E m Ϫ2 s Ϫ1 illumination at canopy height during a 14-h photoperiod (7 am to 9 pm). The average air temperature during the experiment was 23.4°C Ϯ 1.6°C. Both pH and [NH 4 ϩ ] of the nutrient

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Nitrate induction in spruce: an approach using compartmental analysis

Cited by 27 publications

References 40 publications

Differential NO₃⁻ dependent patterns of NO₃⁻ uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

Differential NO₃⁻ dependent patterns of NO₃⁻ uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

The cytosolic Na⁺ : K⁺ ratio does not explain salinity‐induced growth impairment in barley: a dual‐tracer study using ⁴²K⁺ and ²⁴Na⁺

Source and Magnitude of Ammonium Generation in Maize Roots

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Nitrate induction in spruce: an approach using compartmental analysis

Cited by 27 publications

References 40 publications

Differential NO3− dependent patterns of NO3− uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

Differential NO3− dependent patterns of NO3− uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

The cytosolic Na+ : K+ ratio does not explain salinity‐induced growth impairment in barley: a dual‐tracer study using 42K+ and 24Na+

Source and Magnitude of Ammonium Generation in Maize Roots

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Differential NO₃⁻ dependent patterns of NO₃⁻ uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

Differential NO₃⁻ dependent patterns of NO₃⁻ uptake in Pinus pinaster, Rhizopogon roseolus and their ectomycorrhizal association

The cytosolic Na⁺ : K⁺ ratio does not explain salinity‐induced growth impairment in barley: a dual‐tracer study using ⁴²K⁺ and ²⁴Na⁺