“…Nitrate is a known cause of methemoglobinemia, which affects both human and non-human vertebrates, and reduces the capacity of hemoglobin to bind oxygen and carry it through the body (reviewed by Guillette and Edwards, 2005). It has received recent Aquaculture 436 (2015) 8-12 attention as an endocrine disrupting chemical (EDC) due to its effect on reproductive and thyroid hormone systems (Edwards and Guillette, 2007;Edwards et al, 2006;Guillette and Edwards, 2005;Hamlin et al, 2008). Nitrate is a goitrogen and interferes with the production of thyroid hormones (THs) by competitively binding to the sodium/iodide symporter (NIS) on thyroid follicles, decreasing the availability of iodide (I − ) for use in TH synthesis (De Groef et al, 2006;Lahti et al, 1985;Tonacchera et al, 2004).…”