Nitrate assimilation, dissimilatory nitrate reduction to ammonium, and denitrification coexist in Pseudomonas putida Y-9 under aerobic conditions

Huang, Xuejiao; Weisener, Christopher G.; Ni, Jinren; He, Binghui; Xie, Deti; Li, Zhenlun

doi:10.1016/j.biortech.2020.123597

Cited by 104 publications

(43 citation statements)

References 36 publications

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“…P. putida Y-9 (Genbank No. KP410740), conducting both denitrification and DNRA under aerobic conditions [ 15 ], was used in the study.…”

Section: Methodsmentioning

confidence: 99%

“…Strain Y-9 could perform denitrification and DNRA under aerobic conditions [ 15 ]. However, under aerobic conditions, the assimilation of NO 3 − in strain Y-9 is also a major metabolic pathway [ 16 ], which might disturb the study of denitrification and DNRA in strain Y-9.…”

Section: Methodsmentioning

confidence: 99%

“…However, under aerobic conditions, the assimilation of NO 3 − in strain Y-9 is also a major metabolic pathway [ 16 ], which might disturb the study of denitrification and DNRA in strain Y-9. However, when using nitrite as substrate, there was nearly no assimilation observed of NO 2 − in the short time (1 h) whereas the accumulation of NH 4 + reached 1.90 mg/L [ 15 ]. Therefore, NO 2 − instead of NO 3 − was used as a substrate to explore the fate of denitrification and DNRA by strain Y-9 in this study [ 8 ].…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study clarified that both denitrification and DNRA exist in Pseudomonas putida Y-9 under aerobic conditions by genome-wide and gene knockout as well as 15 N isotope technologies [ 15 ]. Given the scarcity of data available to assess the effect of the environmental factors on denitrification and DNRA in a well-defined experimental system, the object of this study is to explore the external factors (carbon source, C/N ratio, pH, and dissolved oxygen) that determine the denitrification and DNRA fate in strain Y-9.…”

Section: Introductionmentioning

confidence: 99%

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Low C/N Ratios Promote Dissimilatory Nitrite Reduction to Ammonium in Pseudomonas putida Y-9 under Aerobic Conditions

et al. 2021

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The biogeochemical consequences of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) have a significant influence on nitrogen (N) cycling in the ecosystem. Many researchers have explored these two pathways in soil and sediment ecosystems under anaerobic conditions. However, limited information is available regarding the influence of external environmental conditions on these two pathways in a well-defined experimental system under aerobic conditions. In this study, the impacts of the external environmental factors (carbon source, C/N ratio, pH, and dissolved oxygen) on nitrite reduction through the denitrification and DNRA routes in Pseudomonas putida Y-9 were studied. Results found that sodium citrate and sodium acetate favored denitrification and DNRA, respectively. Furthermore, neutral pH and aerobic conditions both facilitated DNRA and denitrification. Especially, low C/N ratios motivated the DNRA while high C/N ratios stimulated the denitrification, which was opposite to the observed phenomena under anaerobic conditions.

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“…P. putida Y-9 (Genbank No. KP410740), conducting both denitrification and DNRA under aerobic conditions [ 15 ], was used in the study.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low C/N Ratios Promote Dissimilatory Nitrite Reduction to Ammonium in Pseudomonas putida Y-9 under Aerobic Conditions

et al. 2021

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“…Bacillus and Pseudomonas are the main biocontrol and plant growth-promoting bacteria [ 7 , 8 ], and it is feasible to select bacteria belonging to the genera of Bacillus and Pseudomonas as functional bacteria for secondary soil salinization bioremediation. Many research studies showed that BS, PS and PP have the denitrifying ability [ 9 , 10 , 11 ], so we compared the growth and nitrogen removal ability of these three bacteria, for the purpose of providing reference for selecting better bacteria to repair secondary soil salinization.…”

Section: Introductionmentioning

confidence: 99%

An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal

et al. 2021

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Excessive use of nitrogen fertilizer in intensively managed agriculture has resulted in abundant accumulation of nitrate in soil, which limits agriculture sustainability. How to reduce nitrate content is the key to alleviate secondary soil salinization. However, the microorganisms used in soil remediation cause some problems such as weak efficiency and short survival time. In this study, seaweed polysaccharides were used as stimulant to promote the rapid growth and safer nitrate removal of denitrifying bacteria. Firstly, the growth rate and NO3−-N removal capacity of three kinds of denitrifying bacteria, Bacillus subtilis (BS), Pseudomonas stutzeri (PS) and Pseudomonas putida (PP), were compared. The results showed that Bacillus subtilis (BS) had a faster growth rate and stronger nitrate removal ability. We then studied the effects of Enteromorpha linza polysaccharides (EP), carrageenan (CA), and sodium alginate (AL) on growth and denitrification performance of Bacillus subtilis (BS). The results showed that seaweed polysaccharides obviously promoted the growth of Bacillus subtilis (BS), and accelerated the reduction of NO3−-N. More importantly, the increased NH4+-N content could avoid excessive loss of nitrogen, and less NO2−-N accumulation could avoid toxic effects on plants. This new strategy of using denitrifying bacteria for safely remediating secondary soil salinization has a great significance.

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Cyanobacterial Genomes from a Brackish Coastal Lagoon Reveal Potential for Novel Biogeochemical Functions and Their Evolution

Ray,

Manu,

Rastogi

et al. 2024

J Mol Evol

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Nitrate assimilation, dissimilatory nitrate reduction to ammonium, and denitrification coexist in Pseudomonas putida Y-9 under aerobic conditions

Cited by 104 publications

References 36 publications

Low C/N Ratios Promote Dissimilatory Nitrite Reduction to Ammonium in Pseudomonas putida Y-9 under Aerobic Conditions

Low C/N Ratios Promote Dissimilatory Nitrite Reduction to Ammonium in Pseudomonas putida Y-9 under Aerobic Conditions

An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal

Cyanobacterial Genomes from a Brackish Coastal Lagoon Reveal Potential for Novel Biogeochemical Functions and Their Evolution

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