Nitrate Assimilation by Bacteria

Lin, Jeffrey; Stewart, Valley

doi:10.1016/s0065-2911(08)60014-4

Cited by 165 publications

(143 citation statements)

References 103 publications

Supporting

Mentioning

136

Contrasting

Order By: Relevance

“…Prokaryotes are able to synthesize three distinct types of nitrate reductase that all reduce nitrate to nitrite but serve different physiological functions (for reviews see Lin & Stewart, 1998;Potter et al, 2001;Richardson et al, 2001;González et al, 2006). Two nitrate reductases (Nar and Nap) terminate respiratory electron transport chains, while the cytoplasmic Nas enzyme is involved in assimilatory nitrate reduction.…”

Section: Introductionmentioning

confidence: 99%

Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes

2007

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes

2007

View full text Add to dashboard Cite

“…All three enzymes reduce nitrate to nitrite, but they are involved in different physiological processes (reviewed in Lin and Stewart, 1998;Moreno-Vivian et al, 1999;Potter et al, 2001;Richardson et al, 2001). The Nas enzyme is located in the cytoplasm and participates in nitrogen assimilation, whereas the other two nitrate reductases (Nar and Nap) are both linked to respiratory electron transport systems.…”

Section: Introductionmentioning

confidence: 99%

Deep-sea hydrothermal ventEpsilonproteobacteriaencode a conserved and widespread nitrate reduction pathway (Nap)

et al. 2014

View full text Add to dashboard Cite

Despite the frequent isolation of nitrate-respiring Epsilonproteobacteria from deep-sea hydrothermal vents, the genes coding for the nitrate reduction pathway in these organisms have not been investigated in depth. In this study we have shown that the gene cluster coding for the periplasmic nitrate reductase complex (nap) is highly conserved in chemolithoautotrophic, nitrate-reducing Epsilonproteobacteria from deep-sea hydrothermal vents. Furthermore, we have shown that the napA gene is expressed in pure cultures of vent Epsilonproteobacteria and it is highly conserved in microbial communities collected from deep-sea vents characterized by different temperature and redox regimes. The diversity of nitrate-reducing Epsilonproteobacteria was found to be higher in moderate temperature, diffuse flow vents than in high temperature black smokers or in low temperatures, substrate-associated communities. As NapA has a high affinity for nitrate compared with the membrane-bound enzyme, its occurrence in vent Epsilonproteobacteria may represent an adaptation of these organisms to the low nitrate concentrations typically found in vent fluids. Taken together, our findings indicate that nitrate reduction is widespread in vent Epsilonproteobacteria and provide insight on alternative energy metabolism in vent microorganisms. The occurrence of the nap cluster in vent, commensal and pathogenic Epsilonproteobacteria suggests that the ability of these bacteria to respire nitrate is important in habitats as different as the deep-sea vents and the human body.

show abstract

“…It is transported into the cells by an active transport system and reduced to ammonium by the sequential action of nitrate reductase (NR) and nitrite reductase (NiR) prior to fixation into amino acids through the glutamine synthetase (GS) pathway. The process of nitrite uptake and its regulation has been widely studied in higher plants and fungi (Raghuram et al, 2006;Lochab et al, 2007) algae (Berges, 1997), bacteria (Lin et al, 1998) and to a lesser extent in cyanobacteria (Herrero et al, 2001). Different responses to nitrate have been reported in literature between the non-nitrogen fixing (nitrate utilizing) and nitrogen fixing cyanobacteria.…”

Section: Introductionmentioning

confidence: 99%

Nitrate uptake and nitrite efflux in wild type and Li+-R and Na+-R strains of Spirulina platensis under different nitrogen concentration and light-dark condition

Sharma¹,

Singh²,

Patel³

2012

ABJNA

View full text Add to dashboard Cite

In the present study we have investigated that 4.0gL -1 NaNO 3 concentration is toxic to the growth of wild type Spirulina platensis and its Li + R and Na + R mutant strains. Highest level of extracellular nitrite release was shown by Na + R strain, when grown in 4.0gL -1 NaNO 3 concentration followed by Li + R and wild type strain of S. platensis whereas Li + R strain uptake nitrate more rapidly followed by Na + R and wild type strain of S. platensis, when estimated after 8d of incubation. Cultures assimilate nitrate more rapidly in light than in darkness which suggests that nitrate uptake process depend on photosynthetically generated light energy. The nitrate uptake pattern consisted of two distinct phase : initial rapid phase followed by the slower second phase. There is no lag in the initial uptake of nitrate in either of the culture suggesting that rapid phase is not dependent on reduction of nitrate whereas slower phase seemed to be dependent on metabolism. Among these three strains of S. platensis Li + R strain assimilate nitrate more rapidly and fix it into ammonia by sequential action of nitrate reductase and nitrite reductase.

show abstract

Nitrate Assimilation by Bacteria

Cited by 165 publications

References 103 publications

Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes

Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes

Deep-sea hydrothermal ventEpsilonproteobacteriaencode a conserved and widespread nitrate reduction pathway (Nap)

Nitrate uptake and nitrite efflux in wild type and Li+-R and Na+-R strains of Spirulina platensis under different nitrogen concentration and light-dark condition

Contact Info

Product

Resources

About