Analysis of long-term data from (2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009) in four springs that discharge from the Upper Floridan aquifer into the Flint River (southwestern Georgia, United States) indicate aquifer and surface-water susceptibility to nutrient loading. Nitrate-N concentrations ranged from 1.74 to 3.30 mg ⁄ l, and exceeded historical levels reported for the Upper Floridan aquifer (0.26-1.52 mg ⁄ l). Statistical analyses suggest increasing nitrate-N concentration in groundwater discharging at the springs (n = 146 over eight years) and that nitrate-N concentration is influenced by a dynamic interaction between depth to groundwater (an indicator of regional hydrologic conditions) and land use. A one-time synoptic survey of 10 springs (6 springs in addition to the 4 previously mentioned) using stable isotopes generated d 15 N-NO 3 ) values (4.8-8.4& for rural springs and 7.7-13.4& for developed ⁄ urban springs) suggesting mixed sources (i.e., fertilizer, animal waste, and soil organic nitrogen) of nitrate-N to rural springs and predominantly animal ⁄ human waste to urban springs. These analyses indicate a direct relation between nitrate-N loading since the 1940s and intensification of agricultural and urban land use. This study demonstrates the importance of evaluating long-term impacts of land use on water quality in groundwater springs and in determining how rapidly these changes occur.