Nisin and acid resistance in Salmonella is enhanced by N-dodecanoyl-homoserine lactone

Freitas, Leonardo Luiz de; Santos, Clarissa Isabela Aparecida dos; Carneiro, Deisy Guimarães; Vanetti, Maria Cristina Dantas

doi:10.1016/j.micpath.2020.104320

Cited by 12 publications

(6 citation statements)

References 37 publications

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“…Indole can affect the expression of the AcrB efflux pump and mediate AR through the induction of oxidative stress pathways and phage shock response. Additionally, DSF-and indole-mediated QS have also been reported in S. enterica serovar Typhimurium and are known to play a fundamental role in virulence regulation and AR [10,14,[38][39][40][41][42].…”

Section: Salmonella Enterica Serovar Typhimurium Quorum Sensing Inter...mentioning

confidence: 99%

Polyphenols and CRISPR as Quorum Quenching Agents in Antibiotic-Resistant Foodborne Human Pathogens (Salmonella Typhimurium, Campylobacter jejuni and Escherichia coli 0157:H7)

Higuera-Ciapara,

Benitez-Vindiola,

Figueroa-Yañez

et al. 2024

Foods

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Antibiotic resistance in foodborne pathogens is an increasing threat to global human health. Among the most prevalent antibiotic-resistant bacteria are Salmonella enterica serovar Typhimurium, Campylobacter jejuni and E. coli 0157:H7. Control of these and other pathogens requires innovative approaches, i.e., discovering new molecules that will inactivate them, or render them less virulent without inducing resistance. Recently, several polyphenol molecules have been shown to possess such characteristics. Also, the use of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) approaches has recently been proposed for such purpose. This review summarizes the main findings regarding the application of both approaches to control the above-mentioned foodborne pathogens by relying on Quorum Sensing interference (Quorum Quenching) mechanisms and highlights the avenues needed for further research.

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Section: Salmonella Enterica Serovar Typhimurium Quorum Sensing Inter...mentioning

confidence: 99%

Polyphenols and CRISPR as Quorum Quenching Agents in Antibiotic-Resistant Foodborne Human Pathogens (Salmonella Typhimurium, Campylobacter jejuni and Escherichia coli 0157:H7)

Higuera-Ciapara,

Benitez-Vindiola,

Figueroa-Yañez

et al. 2024

Foods

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“…This study analyzed the main features of the Salmonella Enteritidis PT4 578 genome, a serotype and a phage type of significant clinical relevance and used in several studies of virulence, quorum sensing, and biofilm Carneiro et al, 2020;Byun et al, 2021;Luiz de Freitas et al, 2021;Kim et al, 2022). Furthermore, Salmonella Enteritidis PT4 is predominant in outbreaks in the western states of the United States and is the most frequently isolated from chicken in Europe and South America (Kipper et al, 2022).…”

Section: B C 4 Discussionmentioning

confidence: 99%

“…The sdiA was not influenced by C12-HSL at any of the temperatures. Carneiro et al (2020) observed that C12-HSL also does not influence sdiA expression in Salmonella Enteritidis PT4 planktonic cells at 37 °C incubation in anaerobiosis. However, C12-HSL increased luxS expression at 28 and 37 °C.…”

mentioning

confidence: 83%

“…After 20 h incubation, the culture medium was replaced by a new culture medium supplemented with 50 nmol.L - Conditions used for the amplification were 95 °C for 10 min, 40 denaturing cycles at 95 °C for 30 s, followed by annealing and extension at 60 °C for 1 min. After the 40 cycles, the temperature was gradually increased, by 1 °C increments, to 95 °C, resulting in the generation of a melting curve (Carneiro et al, 2020). The efficiency of amplification was determined by running a standard curve for each primer with serial dilutions DNA and calculated with the formula E=(10 (1/-slope) -1) x 100.…”

Section: Expression Of Genes Related To Biofilm and Quorum Sensingmentioning

confidence: 99%

“…Interestingly, an analysis of the metabolome of planktonic Salmonella Enteritidis PT4 578 grown at 37 °C under anaerobic conditions demonstrated that C12-HSL strongly influenced purine metabolism (Carneiro et al, 2020). Although the physiology of planktonic and sessile cells differes (Joo and Otto, 2012;Giaouris et al, 2013), increased purine metabolism in the presence of C12-HSL may indicate increased c-di-GMP pools.…”

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confidence: 99%

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Whole genome sequencing analysis and biofilm formation in Salmonella Enteritidis PT4 578

Carneiro

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Salmonella is an important and versatile foodborne pathogen responsible for numerous infections and deaths worldwide. The difficulty of controlling and eradicating this pathogen is related to its ability to form complex aggregates of cells, called biofilms. In a biofilm, cells are surrounded by a matrix of self-produced extracellular polymeric substances that protect against various types of stress, such as desiccation, sanitization, and host defense. The Salmonella biofilm matrix is composed mainly of curli fimbriae and cellulose. A highly complex genetic network regulates the synthesis of these components. The main regulators associated with biofilm formation are the CsgD protein and the bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP), which activate the biosynthesis of most polymers. Quorum sensing, a cellular communication mechanism, is also described as an important regulator of biofilm formation in Salmonella. The objective of the present work was to sequence the genome of Salmonella enterica Enteritidis PT4 578, analyze the main characteristics related to virulence and biofilm formation, and compare the genome and morphotype formation, biofilm and motility phenotypes with four related serotypes. Furthermore, evaluate the influence of temperature, atmosphere, and quorum sensing on biofilm formation by this serotype. Genome analyzes showed that Salmonella Enteritidis PT4 578 has 165 virulence genes, representing 3.66% of the coding sequences. Twelve Salmonella pathogenicity islands (SPI) were identified, some extremely conserved, such as SPI1 and SPI2, that encode the apparatus of intracellular invasion and colonization,. A total of thirteen gene clusters related to fimbriae biosynthesis, including the csg operon, were also annotated. Under aerobic and anaerobic conditions, Salmonella Enteritidis PT4 578 can form a biofilm on stainless steel coupons at 28 °C and only under aerobic conditions at 37 °C. However, adding of N-hexanoyl homoserine lactone (C12-HSL) to the anaerobic culture medium reverses the biofilm formation phenotype at 37 °C. In this condition, C12-HSL increased the expression of the adrA gene, a diguanylate cyclase related to the synthesis of c-di-GMP and the luxS gene, which is part of another quorum sensing mechanism that uses autoinducer-2 (AI-2), present in Salmonella. Although it did not influence biofilm formation at 28 ºC, C12- HSL also increased luxS expression. The data presented here reinforce the role of conditions of atmosphere and temperature, as well as quorum sensing in Salmonella biofilm formation. Finally, it reports a possible connection between this pathogen's two main quorum sensing mechanisms. Understanding the factors that influence Salmonella biofilm formation can contribute to developing control and eradication strategies. Keywords: Genome analyses. Biofilm. Quorum sensing.

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Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses

Nguyen

Nguyen-Ho

et al. 2021

Arch Microbiol

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Environmental factors can alter exopolysaccharide biosynthesis in LAB. To further clarify this potential relationship, the mRNA expression of exopolysaccharide-related genes such as glmU, pgmB1, cps4E, cps4F, cps4J and cps4H genes in Lactiplantibacillus plantarum VAL6 under different conditions including high temperature, acidic or alkaline pH, sodium chloride (NaCl) and carbon dioxide (CO 2 ) intensi cation culture was studied. The transcriptomic data revealed that the exposure of L. plantarum VAL6 to acid at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E. Under pH 8 stress, cps4F and cps4E were signi cantly upregulated, whereas pgmB1 was downregulated.Similarly, the expression levels of cps4F, cps4E and cps4J increased sharply under stresses at 42 or 47 o C. In the case of NaCl stress, glmU, pgmB1, cps4J and cps4H were downregulated in exposure to NaCl at 7-10% concentrations while cps4E and cps4F were upregulated at 1 h of 10% NaCl treatment and at 5 h of 4% NaCl treatment. Remarkably, CO 2 intensi cation culture stimulated the expression of all tested genes.In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes. These ndings indicated that the expression level of exopolysaccharide-related genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.

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Nisin and acid resistance in Salmonella is enhanced by N-dodecanoyl-homoserine lactone

Cited by 12 publications

References 37 publications

Polyphenols and CRISPR as Quorum Quenching Agents in Antibiotic-Resistant Foodborne Human Pathogens (Salmonella Typhimurium, Campylobacter jejuni and Escherichia coli 0157:H7)

Polyphenols and CRISPR as Quorum Quenching Agents in Antibiotic-Resistant Foodborne Human Pathogens (Salmonella Typhimurium, Campylobacter jejuni and Escherichia coli 0157:H7)

Whole genome sequencing analysis and biofilm formation in Salmonella Enteritidis PT4 578

Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses

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