NIR-II fluorescence imaging using indocyanine green nanoparticles

Bhavane, Rohan; Starosolski, Zbigniew; Stupin, Igor; Ghaghada, Ketan B.; Annapragada, Ananth

doi:10.1038/s41598-018-32754-y

Cited by 97 publications

(90 citation statements)

References 49 publications

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“…The fact that Cy5 performs this well may be related to its superior brightness relative to ICG caused by a different quantum yield. Optical imaging in the NIR range holds considerable promise due to reduced light scattering by the tissue and increased penetration depth when compared to the visible range [27].…”

Section: Discussionmentioning

confidence: 99%

Multicolor fluorescence imaging using a single RGB-IR CMOS sensor for cancer detection with smURFP-labeled probiotics

Cho

Suh

et al. 2020

Biomed. Opt. Express

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A multicolor fluorescence imaging device was recently developed for image-guided surgery. However, conventional systems are typically bulky and function with two cameras. To overcome these issues, we developed an economical home-built fluorescence imaging device based on a single RGB-IR sensor that can acquire both color and fluorescence images simultaneously. The technical feasibility of RGB-IR imaging was verified ex vivo in chicken breast tissue using fluorescein isothiocyanate (FITC), cyanine 5 (Cy5), and indocyanine green (ICG) as fluorescent agents. The minimum sensitivities for FITC, Cy5, and ICG were 0.200 µM, 0.130 µM, and 0.065 µM, respectively. In addition, we validated the fluorescence imaging of this device in vitro during a minimally invasive procedure using smURFP-labeled probiotics, which emit a spectrum similar to that of Cy5. Our preliminary study of the ex vivo tissue suggests that Cy5 and ICG are good candidates for deep tissue imaging. In addition, the tumor-specific amplification process was visualized using cancer cells incubated with probiotics that had been labeled with a fluorescent protein. Our approach indicates the potential for in vivo screening of tumors in rodent tumor models.

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Section: Discussionmentioning

confidence: 99%

Multicolor fluorescence imaging using a single RGB-IR CMOS sensor for cancer detection with smURFP-labeled probiotics

Cho

Suh

et al. 2020

Biomed. Opt. Express

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“…Clinical transformation of NIR-II imaging technology can be accelerated when ICG, an FDA approved imaging agent, is used. Later, the research team published another paper on NIR-II fluorescence imaging using indocyanine green nanoparticles (Bhavane et al, 2018). In this study, they collected the fluorescence spectra of ICG liposomes in PBS and plasma.…”

Section: Nanoparticles Based On Organic Fluorescent Dyesmentioning

confidence: 99%

Recent Progress in NIR-II Contrast Agent for Biological Imaging

Cao

Zhu

Zheng

et al. 2020

Front. Bioeng. Biotechnol.

208

190

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Fluorescence imaging technology has gradually become a new and promising tool for in vivo visualization detection. Because it can provide real-time sub-cellular resolution imaging results, it can be widely used in the field of biological detection and medical detection and treatment. However, due to the limited imaging depth (1-2 mm) and self-fluorescence background of tissue emitted in the visible region (400-700 nm), it fails to reveal biological complexity in deep tissues. The traditional near infrared wavelength (NIR-I, 650-950 nm) is considered as the first biological window, because it reduces the NIR absorption and scattering from blood and water in organisms. NIR fluorescence bioimaging's penetration is larger than that of visible light. In fact, NIR-I fluorescence bioimaging is still interfered by tissue autofluorescence (background noise), and the existence of photon scattering, which limits the depth of tissue penetration. Recent experimental and simulation results show that the signal-to-noise ratio (SNR) of bioimaging can be significantly improved at the second region near infrared (NIR-II, 1,000-1,700 nm), also known as the second biological window. NIR-II bioimaging is able to explore deep-tissues information in the range of centimeter, and to obtain micron-level resolution at the millimeter depth, which surpass the performance of NIR-I fluorescence imaging. The key of fluorescence bioimaging is to achieve highly selective imaging thanks to the functional/targeting contrast agent (probe). However, the progress of NIR-II probes is very limited. To date, there are a few reports about NIR-II fluorescence probes, such as carbon nanotubes, Ag 2 S quantum dots, and organic small molecular dyes. In this paper, we surveyed the development of NIR-II imaging contrast agents and their application in cancer imaging, medical detection, vascular bioimaging, and cancer diagnosis. In addition, the hotspots and challenges of NIR-II bioimaging are discussed. It is expected that our findings will lay a foundation for further theoretical research and practical application of NIR-II bioimaging, as well as the inspiration of new ideas in this field.Keywords: fluorescence imaging technology, the second region near infrared (NIR-II), biological imaging, contrast agents, biomedical applications Frontiers in Bioengineering and Biotechnology | www.frontiersin.org

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“…NIR-II fluorescence imaging has received extensive attentions after the first use of in vivo NIR-II fluorescence imaging in mice in 2009. [3,26] Currently, various materials, such as carbon nanotubes, [20,27,28] quantum dots, [29][30][31][32] rare-earth nanoparticles, [33][34][35][36][37][38] organic small molecules, [39][40][41][42][43][44][45][46][47][48][49][50][51][52] and polymers, [53,54] have been applied in NIR-II fluorescence bioimaging. Although several reviews of NIR-II fluorescence probe for bio medical imaging have been reported, [3,6,11,15,18,24,55,56] currently no review article focuses on activatable NIR-II fluorescence probes since the first activatable NIR-II fluorescence probe was reported in 2013.…”

Section: "Always On" Versus Activatable Fluorescence Probesmentioning

confidence: 99%

Recent Advances on Activatable NIR‐II Fluorescence Probes for Biomedical Imaging

Tang

Pei

Lü

et al. 2019

Advanced Optical Materials

125

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Newly emerging fluorescence imaging in the second near‐infrared window (NIR‐II, 1000–1700 nm) permits visualization of deep anatomical features with unprecedented spatial resolution due to its minimized autofluorescence, optical scattering and absorption of tissue, and increased applicable power at longer wavelengths than the traditional NIR (NIR‐I, 650–900 nm) fluorescence imaging. Compared with “always‐on” NIR‐II fluorescence probes, activatable NIR‐II fluorescence probes that can change their fluorescence signals by pathologic parameters stimuli, hold great promise for providing high target‐to‐background ratio, significantly improving specificity and sensitivity of early disease detection. Additionally, such probes provide an excellent opportunity to understand the underlying pathological mechanism at the molecular level in order to optimize therapeutic interventions. Here, the recent advances in the development of activatable NIR‐II fluorescence probes for bioimaging and biosensing are reviewed. The photochemical design mechanisms and biomedical applications of these probes are described in detail. Moreover, the present challenges to completely exhibit the potential of activatable NIR‐II fluorescence probes for optical theranostics in life science are also discussed. Finally, the future perspective is further analyzed.

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NIR-II fluorescence imaging using indocyanine green nanoparticles

Cited by 97 publications

References 49 publications

Multicolor fluorescence imaging using a single RGB-IR CMOS sensor for cancer detection with smURFP-labeled probiotics

Multicolor fluorescence imaging using a single RGB-IR CMOS sensor for cancer detection with smURFP-labeled probiotics

Recent Progress in NIR-II Contrast Agent for Biological Imaging

Recent Advances on Activatable NIR‐II Fluorescence Probes for Biomedical Imaging

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