Niobium-Doped Hydroxyapatite Bioceramics:  Synthesis, Characterization and In Vitro Cytocompatibility

Capanema, Nádia S.V.; Mansur, Alexandra A.P.; Carvalho, Sandhra M.; Silva, Alexandra R P; Ciminelli, Virgínia S. T.

doi:10.3390/ma8074191

Cited by 39 publications

(19 citation statements)

References 42 publications

(68 reference statements)

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“…These structural modifications can alter the solubility, thermal stability, and mechanical properties as well as the in-vitro bioactivity of HA crystals [16][17][18][19]. Over the past decades, many different methods have been introduced for the production of various substituted apatites with precise control of the microstructure, particle shape and size [20][21][22][23][24]. Among them, powders synthesized by mechanochemical reactions typically possess a well-defined structure due to the perturbation of surface-bonded species as a result of pressure, which enhances the thermodynamic and kinetic reactions between solids [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Structural insights of mechanically induced aluminum-doped hydroxyapatite nanoparticles by Rietveld refinement

Fahami

Beall

Basirun

2017

Chinese Journal of Chemical Engineering

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Aluminum doped hydroxyapatite (HA:Al 3+) nanopowders were successfully prepared via a simple and efficient one-pot mechanochemical route. The effects of dopant loading on phase compositions and structural features were assessed by Rietveld analysis. The XRD-Rietveld refinement revealed the stabilization of HA in hexagonal structure for all the samples. The sharpness and intensity of the apatite-derived XRD peaks decreased as the dopant content increased to 10% due to the increase in lattice imperfections and mechanically induced amorphization. The incorporation of Al 3+ into the HA lattice decreased the unit cell parameters. From the FTIR measurements, the representing bands of apatite were identified in all cases. The mechanosynthesized nanopowders consisted of nanospheroids with an average size of 44±20 nm and therefore are promising for bone tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Structural insights of mechanically induced aluminum-doped hydroxyapatite nanoparticles by Rietveld refinement

Fahami

Beall

Basirun

2017

Chinese Journal of Chemical Engineering

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“…Several other studies have also shown that the addition of niobium into biomaterials do not result in cytotoxicity. These investigations include tests with different materials, such as fluorapatite glass‐ceramics (Kushwaha et al, ), phosphate glasses (Minghui, Wenchuan, Jun, et al, ), and bioactive glass‐niobium granules (Fernandes et al, 2012) hydroxyapatite bioceramics (Capanema et al, ) and calcium silicate cements with different cell lines (Mestieri et al, ). In one of these assays, human osteogenic cells (SAOS) were incubated with Nb‐doped hydroxyapatite for 72 h and cell viability was tested by means of a MTT assay.…”

Section: Resultsmentioning

confidence: 99%

“…In one of these assays, human osteogenic cells (SAOS) were incubated with Nb‐doped hydroxyapatite for 72 h and cell viability was tested by means of a MTT assay. They observed no difference or even a statistically significant increase in cell proliferation when compared to the control group (reference material) which allowed them to conclude that Nb‐doped hydroxyapatite has the ability to support cell proliferation (Capanema et al, ). Furthermore, a very recent investigation evaluated the cytocompatibility of calcium silicate‐based cements combined with Nb 2 O 5 micro and nanoparticles, comparing the response in four different cell lines: (a) human dental pulp cells—hDPCs; (b) human dental follicle cells—hDFCs; (c) human osteoblast‐like cells (SAOS‐2); and (d) mouse periodontal ligament cells—mPDL.…”

Section: Resultsmentioning

confidence: 99%

In vitro and in vivo osteogenic potential of niobium‐doped 45S5 bioactive glass: A comparative study

et al. 2019

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In vitro and in vivo experiments were undertaken to evaluate the solubility, apatite‐forming ability, cytocompatibility, osteostimulation, and osteoinduction for a series of Nb‐containing bioactive glass (BGNb) derived from composition of 45S5 Bioglass. Inductively coupled plasma optical emission spectrometry (ICP‐OES) revealed that the rate at which Na, Ca, Si, P, and Nb species are leached from the glass decrease with the increasing concentration of the niobium oxide. The formation of apatite as a function of time in simulated body fluid was monitored by 31P Magic Angle Spinning (MAS) Nuclear magnetic resonance spectroscopy. Results showed that the bioactive glasses: Bioglass 45S5 (BG45S5) and 1 mol%‐Nb‐containing‐bioactive glass (BGSN1) were able to grow apatite layer on their surfaces within 3 h, while glasses with higher concentrations of Nb2O5 (2.5 and 5 mol%) took at least 12 h. Nb‐substituted glasses were shown to be compatible with bone marrow‐derived mesenchymal stem cells (BMMSCs). Moreover, the bioactive glass with 1 mol% Nb2O5 significantly enhanced cell proliferation after 4 days of treatment. Concentrations of 1 and 2.5 mol% Nb2O5 stimulated osteogenic differentiation of BMMSCs after 21 days of treatment. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their osteoconductivity and osteostimulation. Two morphometric parameters were analyzed: (a) thickness of new‐formed bone layer and (b) area of new‐formed subperiostal bone. Results showed that BGNb bioactive glass is osteoconductive and osteostimulative. Therefore, these results indicate that Nb‐substituted glass is suitable for biomedical applications.

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“…In recent years, many foreign ions were incorporated in trace levels, either in the HAP crystal lattice or adsorbed on the crystal surface and, such modifications have led to a significant influence on the stoichiometry, crystallinity, crystallite size, and morphological features of HAP . The most common trace element or ionic moiety having a potential to improve physical, chemical, and biological properties upon incorporation in the HAP matrix are Mg 2+ , Zn 2+ , Sr 2+ , CO 3 2− , F − , etc . Incorporation of these elements plays a substantial role in improving the biological activity of the apatites and the eventual bone formation and regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5][6] The most common trace element or ionic moiety having a potential to improve physical, chemical, and biological properties upon incorporation in the HAP matrix are Mg 2+ , Zn 2+ , Sr 2+ , CO 2À 3 , F À , etc. [7][8][9][10][11][12][13][14][15] Incorporation of these elements plays a substantial role in improving the biological activity of the apatites and, the eventual bone formation and regeneration.…”

Section: Introductionmentioning

confidence: 99%

Crystalline selenite substituted carbonated hydroxyapatite nanorods: Synthesis, characterization, evaluation of bioactivity and cytotoxicity

Rajendran

Ravichandran

Nellaiappan

2016

Int J Applied Ceramic Tech

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Synthesis of pure and selenite substituted hydroxyapatites (HAP and Se-HAP with 0.02-0.10Se) by sol-gel method and evaluation of their morphological features, nature of functional groups, phase purity, in vitro bioactivity and cytotoxicity are addressed. Spectral studies confirm the incorporation of selenite into the HAP lattice, accompanied by an increase in CO 3 2-content to maintain the charge imbalance following replacement of P 5+ with Se 4+, thus making the formation of selenite substituted carbonated HAP. Selenite substitution in the HAP lattice has led to a higher crystallinity and increased the crystallite size. The morphology of HAP is changed from sphere to rod-like structure upon substitution by selenite and the size of the rod is increased with an increase in the selenite content.Among the Se-HAP's, a better in vitro bioactivity and cell viability are observed for 0.02Se-HAP and 0.04Se-HAP while the trend is reversed when the extent of selenite substitution becomes higher. role in improving the biological activity of the apatites and, the eventual bone formation and regeneration. Selenium is a vital micro-nutrient and one of the most essential elements required for many biological metabolisms. It plays an important role in the protein functions, induction of cancer cell apoptosis, proliferation, and differentiation of bone cells to improve bone health. It also exhibits good antibacterial property. [16][17][18][19][20] Thus, substitution of selenite in the HAP matrix is likely to improve biological functions. However, studies on selenite incorporated HAPs are rather limited. The biological influence of selenium on human system is dose dependant and hence the use of selenium beyond the tolerance level could lead to selenosis and toxicity to cellular environment. [21][22][23] Hence, it is imperative to optimize the concentration of selenite in HAP. In earlier reports, selenite-substituted HAP (Se-HAP) was synthesized by precipitation method. 16,17,24,25 Nevertheless, this method led to a decrease in crystallinity of Se-HAP and promoted agglomeration of crystals even at lower concentrations of selenite. Crystalline HAP's are known to improve biomineralization process (the bone like apatite formation) and hence, they serve as exceptional biomaterials. Moreover, orthopedic implant applications warrant the use of highly crystalline apatites due to their stability during the early stages of resorption process and their ability to increase the longevity of the coating. 26Among the various methods available for the synthesis of HAP, 27-33 the sol-gel method has received much attention owing to its inherent advantages such as high product purity, improved crystallinity, homogenous molecular mixing and, its ability to synthesize HAP's at relatively low temperatures. 34,35 The sol-gel method, which has not been attempted previously for the synthesis of Se-HAP is an ideal choice to prepare high pure crystalline apatites. 36-38The present study aims to synthesize Se-HAP's with varying precursor selenite co...

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Niobium-Doped Hydroxyapatite Bioceramics: Synthesis, Characterization and In Vitro Cytocompatibility

Cited by 39 publications

References 42 publications

Structural insights of mechanically induced aluminum-doped hydroxyapatite nanoparticles by Rietveld refinement

Structural insights of mechanically induced aluminum-doped hydroxyapatite nanoparticles by Rietveld refinement

In vitro and in vivo osteogenic potential of niobium‐doped 45S5 bioactive glass: A comparative study

Crystalline selenite substituted carbonated hydroxyapatite nanorods: Synthesis, characterization, evaluation of bioactivity and cytotoxicity

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