Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins

Shivakumar, Anantharaman; Nagaraja, Padmarajaiah; Al-Tayar, Naef Ghllab Saeed; Shrestha, Ashwinee Kumar

doi:10.1016/j.saa.2016.10.040

Cited by 18 publications

(5 citation statements)

References 33 publications

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“…29 However, it would be too inaccurate for amino-modified cottons to quantify amino groups by titration since cottons could not be dissolved in water. Therefore, ninhydrin was used to dye cotton fibers 8 (cotton-NH 2 ) to provide additional conformation, showing the expected color change of yellow to brown, which was a result of the proverbial chromogenic reaction 42 (SI, Figure S3).…”

Section: Surface Analysis Of Cotton Fibersmentioning

confidence: 99%

Ag NP-Loaded Cotton Fiber Materials: Preparation, Surface Deposition, and Antibacterial Activity with Different Chemical Structures

Pan

Yang

Moloney

et al. 2018

ACS Appl. Bio Mater.

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An amino-substituted bis(aryldiazomethane) was synthesized and used to modify cotton fibers by a carbene insertion reaction. A subsequent amidation of the modified cotton fibers introduced thioether groups onto the surface. Thus, three different types of cotton fibers with hydroxyl, amine, or thioether groups were expected to bind silver nanoparticles (Ag NPs), which were characterized by scanning electron microscopy to show the surface morphology, as well as X-ray photoelectron spectroscopy, energy-dispersive X-ray spectroscopy, and thermogravimetric analysis to quantify the loading of Ag NPs. A bioassay experiment showed antibacterial activity of the modified cotton fibers. The development of these antibacterial cotton fibers not only gave a clear and deep comparison of different silver-binding approaches but also had potential industrial and daily applications for medical textiles.

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Section: Surface Analysis Of Cotton Fibersmentioning

confidence: 99%

Ag NP-Loaded Cotton Fiber Materials: Preparation, Surface Deposition, and Antibacterial Activity with Different Chemical Structures

Pan

Yang

Moloney

et al. 2018

ACS Appl. Bio Mater.

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“…To evaluate the differential proximate components of propolis, bee bread and royal jelly, Fourier transform infrared (FTIR) spectroscopy was performed utilising Bruker FTIR spectrophotometer (Germany) in the range of 4000‐400 cm −1 . The standardised in vitro protocols proposed in the literature were used to evaluate moisture content, ash content, total phenol (Ainsworth & Gillespie, 2007), total carbohydrate (Masuko et al ., 2005), total protein (Anantharaman et al ., 2017), total lipid (Mishra et al ., 2014), protein digestibility and total energy (Sommano et al ., 2020) of bee products.…”

Section: Methodsmentioning

confidence: 99%

Proximate analysis, nutritional quality and anti‐amylase activity of bee propolis, bee bread and royal jelly

Fallah

Najafi

Kavoosi

2022

Int J of Food Sci Tech

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This study discussed the proximate composition, nutritional properties, amino acid profile and antiamylase activity of royal jelly, propolis and bee bread. Royal jelly, bee bread and propolis have lower protein and lipid and more sugar than soybeans and eggs. Soybeans have the highest energy, followed by bee bread, propolis, royal jelly and eggs. Eggs have the highest protein digestibility, followed by royal jelly, bee bread, soybeans and propolis. The primary amino acids in bee bread, royal jelly and propolis are proline, glycine, lysine, alanine, aspartic acid, serine, glutamic acid, threonine, arginine, leucine, tyrosine, phenylalanine, histidine, b-aminobutyric acid, valine, asparagine and methionine. Amino acids from royal jelly, propolis and bee bread inhibited amylase by increasing Km/Vmax and decreasing Vmax and Km through un-competitive or non-competitive strategies. Molecular docking, ultraviolet absorption and fluorescence quenching analysis showed that amino acids interact with amylase structure at the allosteric site through van der Waals interactions and hydrogen bonds. Bee products can be used as food supplements, ingredients and medications to manage diabetes by inhibiting amylase.

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“…The activities of CAT, POD, and SOD in cotton leaves were measured according to the Guaiacol method, NBT photochemical reduction method, and UV absorption method. The contents of H 2 O 2 , Pro, and MDA were measured according to the ammonium molybdate method [51], sulfur barbituric acid method [87], and ninhydrin color development method [88]. Soluble protein and soluble sugar were determined according to the anthrone colorimetry and Coomassie brilliant blue (G-250) methods described by Wu et al [89].…”

Section: Variable Determinationsmentioning

confidence: 99%

Mycorrhizas Affect Physiological Performance, Antioxidant System, Photosynthesis, Endogenous Hormones, and Water Content in Cotton under Salt Stress

Zhang,

Tong,

Wang

et al. 2024

Plants

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Saline–alkali stress seriously endangers the normal growth of cotton (Gossypium hirsutum). Arbuscular mycorrhizal fungi (AMF) could enhance salt tolerance by establishing symbiotic relationships with plants. Based on it, a pot experiment was conducted to simulate a salt environment in which cotton was inoculated with Paraglomus occultum to explore its effects on the saline–alkali tolerance of cotton. Our results showed that salt stress noticeably decreased cotton seedling growth parameters (such as plant height, number of leaves, dry weight, root system architecture, etc.), while AMF exhibited a remarkable effect on promoting growth. It was noteworthy that AMF significantly mitigated the inhibitory effect of salt on cotton seedlings. However, AMF colonization in root and soil hyphal length were collectively descended via salt stress. With regard to osmotic regulating substances, Pro and MDA values in roots were significantly increased when seedlings were exposed to salt stress, while AMF only partially mitigated these reactions. Salt stress increased ROS levels in the roots of cotton seedlings and enhanced antioxidant enzyme activity (SOD, POD, and CAT), while AMF mitigated the increases in ROS levels but further strengthened antioxidant enzyme activity. AMF inoculation increased the photosynthesis parameters of cotton seedling leaves to varying degrees, while salt stress decreased them dramatically. When inoculated with AMF under a salt stress environment, only partial mitigation of these photosynthesis values was observed. Under saline–alkali stress, AMF improved the leaf fluorescence parameters (φPSII, Fv′/Fm′, and qP) of cotton seedlings, leaf chlorophyll levels, and root endogenous hormones (IAA and BR); promoted the absorption of water; and maintained nitrogen balance, thus alleviating the damage from salt stress on the growth of cotton plants to some extent. In summary, mycorrhizal cotton seedlings may exhibit mechanisms involving root system architecture, the antioxidant system, photosynthesis, leaf fluorescence, endogenous hormones, water content, and nitrogen balance that increase their resistance to saline–alkali environments. This study provide a theoretical basis for further exploring the application of AMF to enhance the salt tolerance of cotton.

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Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins

Cited by 18 publications

References 33 publications

Ag NP-Loaded Cotton Fiber Materials: Preparation, Surface Deposition, and Antibacterial Activity with Different Chemical Structures

Ag NP-Loaded Cotton Fiber Materials: Preparation, Surface Deposition, and Antibacterial Activity with Different Chemical Structures

Proximate analysis, nutritional quality and anti‐amylase activity of bee propolis, bee bread and royal jelly

Mycorrhizas Affect Physiological Performance, Antioxidant System, Photosynthesis, Endogenous Hormones, and Water Content in Cotton under Salt Stress

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