Ninhydrin reactive contaminants from Dowex-50 cation exchange resins

Greenland, Robert D.; Roth, William; Gieske, T. H.; Michaelson, I.Arthur

doi:10.1016/0003-2697(74)90393-5

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…The fraction eluted with 2 M-NH3 was evaporated and washed three times with water to remove the NH3 and hydrolysed for amino acid analysis. Control experiments were performed to determnine base-line values arising from residual buffer comnponents or from the AG-50 resin (Greenland et al, 1974).…”

Section: Fractionation Ofhair Proteinsmentioning

confidence: 99%

Loss of a homologous group of proteins in a dominantly inherited ectodermal malformation

Tenenhouse¹,

Gold²

1976

Biochemical Journal

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Hair from mice bearing the dominantly inherited Naked trait (NN) and from normal (NN) mice of the same inbred strain was separated into its major protein components by standard techniques. The relative amounts of proteins in these components were then determined by a regression method from the amino acid composition of the hair samples and of the fractions into which they had been separated. The results indicated that the amount of soluble fibril in Naked-mouse hair is decreased. Polyacrylamide-gel electrophoresis of this fraction prepared from the hair of both normal and Naked mice revealed that all protein bands present in the normal are also present in the Naked mice. However, a densitometric scan of the gels at 280 nm showed that the soluble fibril fraction from Naked-mouse hair is deficient in several proteins which, on amino acid analysis, were found to contain 31% glycine and 10% tyrosine. Gel filtration of S-carboxymethylkerateine prepared from normal and mutant hair showed that the mutant hair is deficient in a heterogeneous, low-molecular-weight fraction also rich in glycine and tyrosine. Our present data do not reveal the mechanism whereby a single gene locus modulates the production of several different proteins.

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Section: Fractionation Ofhair Proteinsmentioning

confidence: 99%

Loss of a homologous group of proteins in a dominantly inherited ectodermal malformation

Tenenhouse¹,

Gold²

1976

Biochemical Journal

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“…The "internal" and "external" porosity of a resin packed in a column is measured by the retention of co-ions and counterions (53). Ninhydrin-positive impurities were leached by ammonia from highly-purified cation-exchange resins (173).…”

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confidence: 99%

Ion exchange and liquid column chromatography

Walton¹

1976

Anal. Chem.

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This year the reviews of ion exchange and of liquid column chromatography are combined. It was logical to do this, for the techniques of high-performance liquid .chromatography include the use of ion exchangers. For one reviewer to report on two years' advances in liquid chromatography as well as in ion exchange is, however, a formidable task. It was made somewhat easier by the decision to include ion-exchanging thin-layer and paper chromatography in another review. "Liquid ion exchangers" are likewise omitted except for a few references to partition chromatography.To cite all the literature references in the two overlapping fields of ion exchange and liquid column chromatography is clearly impossible. Computer print-outs can be obtained from the abstract services, but a reviewer should do more than merely copy these lists. I preferred to peruse the Harold F. Walton joined the staff of the University of Colorado in 1947. His research interests in ion exchange date from 1938 when he went to work for the Permutit Co. as a research chemist; from there he went to Northwestern University in 1940. He obtained the BA and DPhil degrees at Oxford University. He is the author of three textbooks on inorganic and analytical chemistry and coauthor with William Rieman III of "Ion Exchange in Analytical Chemistry" published in 1970. He has contributed chapters on the physical and analytical chemistry of ion exchange to several cooperative works. In 1961 he was chairman of the Gordon Research Conference on Ion Exchange. Dr. Walton spent the 1966-67 academic year and half of 1970 as a Fulbright visiting professor at the University of Trujillo, Peru. He was a member of the Advisory Board of Analytical Chemistry.

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