Nile tilapia (Oreochromis niloticus) as an aquatic vector for Pseudomonas species of medical importance: Antibiotic Resistance Association with Biofilm Formation, Quorum Sensing and Virulence

Osman, Kamelia M.; Pires, Állan da Silva; Franco, Octávio Luiz; Saad, Alaa; Hamed, Mai; Naim, Heba; Ali, Amr H.M.; Elbehiry, Ayman

doi:10.1016/j.aquaculture.2020.736068

Cited by 18 publications

(8 citation statements)

References 79 publications

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“…The observed clinical signs and PM lesions were similar to that reported by (McCarthy et al, 2013;Kumar et al, 2015;Mohammed, 2017;Ummey et al, 2020). The exhibition of virulence factors and formation of biofilms are the major factors for the establishment of disease in aquaculture animals by the bacterial pathogens (Osman et al, 2020). Isolation and identification of isolated strains based on the colonial characters on selective media and the results of the biochemical reactions.…”

Section: Discussionsupporting

confidence: 85%

Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes

El-Sharaby¹,

El-Ballal²,

Ali³

et al. 2021

Journal of Current Veterinary Research

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One of the major threats to aquaculture sector is bacterial diseases. In the current study, samples from different species of cultured fishes were collected from different fish farms at different localities in Menofiya and Kafr El-Sheikh Provinces, Egypt for the detection of causative agents of some disease. Clinically, the infected fishes were inspected for symptoms like darkness of skin and, hemorrhages in different parts of the fish's body including the base of fins, tail, and rot of fins. Identification of the retrieved bacterial pathogens using conventional biochemical and molecular methods were demonstrated. Based on the phenotypic and biochemical characterization using API20E, API 20 NE and VITEK® 2 compact, the retrieved isolates were identified as Yersinia ruckeri and Psychrobacter species. Molecular characterization was accomplished through polymerase chain reaction (PCR) and confirmed by DNA-sequencing and phylogenetic analysis. The high molecular identity and close phylogenetic relationship confirmed that the isolates were genetically homologous to Yersinia ruckeri and Psychrobacter spp.

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Section: Discussionsupporting

confidence: 85%

Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes

El-Sharaby¹,

El-Ballal²,

Ali³

et al. 2021

Journal of Current Veterinary Research

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“…Pathogens such as Aeromonas spp., Pseudomonas spp. and several Enterobacteriaceae are frequently reported in aquaculture, causing disease in fish and other aquatic animals [ 20 , 21 ]. Hence, food-animals have been pointed out as reservoirs of ARGs and antibiotic-resistant pathogens [ 22 ], which can be transferred to humans through the food chain.…”

Section: Introductionmentioning

confidence: 99%

Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure

Almeida

Tacão

Soares

et al. 2021

IJERPH

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The emergence of antibiotic-resistant pathogens due to worldwide antibiotic use is raising concern in several settings, including aquaculture. In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytetracycline (OTC) for two months, followed by a recovery period. The selection of ARB in water and fish was determined using selective media. The abundance of tetA genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control samples, although statistical significance was only achieved five days after exposure. Isolates recovered from samples exposed to the antibiotic were affiliated with Pseudomonas and Stenotrophomonas. Various antibiotic susceptibility profiles were detected and 37% of the isolates displayed multidrug resistance (MDR). The selection of the tetA gene was confirmed by qPCR at the highest OTC concentration tested. Two MDR isolates, tested using zebrafish embryos, caused significant mortality, indicating a potential impact on fish health and survival. Overall, our work highlights the potential impact of antibiotic contamination in the selection of potential pathogenic ARB and ARGS.

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“…In recent years in Egypt, the high mortality and morbidity rates recorded in freshwater fish farms were due to the prevalence of bacterial diseases that are concomitant with water temperature in summer (Enany et al, 2019). In Egypt, Osman et al (2021) reported that Pseudomonas sp. Isolated from Nile tilapia farmed or wild captured (River Nile) were carried antibiotic resistance, Quorum sensing, and virulence genes.…”

Section: Introductionmentioning

confidence: 99%

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2022

JJBS

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Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell a n d tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscripts that meet the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately in one to two months after acceptance.

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Nile tilapia (Oreochromis niloticus) as an aquatic vector for Pseudomonas species of medical importance: Antibiotic Resistance Association with Biofilm Formation, Quorum Sensing and Virulence

Cited by 18 publications

References 79 publications

Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes

Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes

Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure

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