NIH ImageJ and Slice‐O‐Matic Computed Tomography Imaging Software to Quantify Soft Tissue

Abstract

Research Methods and Procedures:Abdominal and midthigh images were obtained using single-slice computed tomography. Two trained investigators analyzed each computed tomography image in duplicate. Adipose tissue and skeletal muscle cross-sectional areas (centimeters squared) were calculated using standard Hounsfield unit ranges (adipose tissue: Ϫ190 to Ϫ30 and skeletal muscle: Ϫ29 to 150). Regions of interest included abdominal total area, total fat area, subcutaneous fat area, visceral fat area (AVF), and right and left thigh total area, fat area, and skeletal muscle area. Results: For all images, intra-investigator coefficients of variation ranged from 0.2% to 3.4% and from 0.4% to 5.6% and inter-investigator coefficients of variation ranged from 0.9% to 4.8% and 0.2% to 2.6% for Slice-O-Matic and NIH ImageJ, respectively, with intra-and inter-investigator coefficients of reliability of R 2 ϭ 0.99. Mean AVF values for investigators A and B ranged from 168 to 170 cm 2 using Slice-O-Matic and NIH ImageJ. Bland-Altman analyses revealed that Slice-O-Matic and NIH ImageJ results were comparable. The mean differences (95% confidence intervals) between the AVF cross-sectional areas obtained using the Slice-O-Matic and NIH ImageJ medical imaging software were ϩ2.5 cm 2 (Ϫ5.7, ϩ10.8 cm 2 ) or ϩ1.4% (Ϫ3.4%, ϩ6.4%). Discussion: These findings show that both the Slice-OMatic and NIH ImageJ medical imaging software systems provide reliable measurements of adipose tissue and skeletal muscle cross-sectional areas.

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