Nigrosphaerin A a new isochromene derivative from the endophytic fungus Nigrospora sphaerica

Abstract

Nigrosphaerin A, a new isochromene derivative (1), was isolated from the endophytic fungus Nigrospora sphaerica and chemically identified as 3-(3,4-dihydroxyphenyl)-4,6,8-trihydroxy-1H-isochromen-1-one-6-O-β-d-glucopyranoside. In addition nineteen known compounds (2–20) were isolated from the same fungus and chemically identified. Compounds (1–3, 5, and 7–16) were isolated for the first time from this fungus. In vitro antileukemic, antileishmanial, antifungal, antibacterial and antimalarial activities of (1–20) were examined. Compounds 5, 7, 9 and 10 showed good antileukemic activity against HL60 cells with IC50 values of 0.03, 0.39, 0.2 and 0.4 μg/mL, respectively and against K562 cells with IC50 values of 0.35, 0.35, 0.49 and 0.01 μg/mL, respectively. Compounds 3, 4 and 6 showed moderate antileishmanial activity with IC50 values of 30.2, 26.4 and 36.4 μg/ml, respectively. Compound 7 showed moderate antifungal activity against Cryptococcus neoformans with IC50 value of 14.8 μg/mL.

Production and characterization of bioactive metabolites from piezotolerant deep sea fungusNigrosporasp. in submerged fermentation

“…), isochromene derivative (Metwaly et al . ), furanone analogues (Zhang et al . ) and alkaloids (Dong et al .…”

Acremonoside, a phenolic glucoside from the sea fan-derived fungus Acremonium polychromum PSU-F125

“…Thus, the sugar unit would be a b-glucopyranoside. The bglucopyranoside moiety was confirmed by direct comparison of the hydrolysate of 1 with an authentic D-(+)-glucose by co-TLC (Metwaly et al, 2014). Based on the X-ray data of 1 (Fig.…”

Anti-leishmanial and Anti-inflammatory Agents from Endophytes: A Review

“…Recently, in a preliminary screening to search potential antileishmanial compounds, Cota et al [34] identified ethyl acetate extract of the fungus Nectria pseudotrichia, an endophyte of Caesalpinia echinata as promising against Leishmania braziliensis. The fractionation led to seven compounds including 10-acetyl trichoderonic acid A (21), 6′-acetoxy-piliformic acid (22), 5′,6′-dehydropiliformic acid (23), piliformic acid (24), hydroheptelidic acid (25), xylaric acid D (26), and cytochalasin D (27) [36]. Similarly, three new azaphilones named mycoleptones A, B, and C (36)(37)(38) along with austdiol (39), eugenitin (40), 6-methoxyeugenin (41), and 9-hydroxyeugenin (42), were also isolated from extract of Mycoleptodiscus indicus, a fungus associated with the medicinal plant Borreria verticillata.…”