NIGMS Human/Rodent Somatic Cell Hybrid Mapping Panels 1 and 2

Drwinga, Helen L.; Toji, Lorraine; Kim, C.H.; Greene, A.E.; Mulivor, R.A.

doi:10.1006/geno.1993.1190

Cited by 134 publications

(54 citation statements)

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“…Several genes linked to agouti in mouse chromosome 2 map to the long arm of human chromosome 20 (14,15). In an attempt to determine whether clone h20B1 also maps to human chromosome 20, PCR was performed on 24 DNA samples from the NIOMS Cell Repository human-rodent somatic cell hybrid panel 2 (24). Primers for this experiment overlapped the region homologous to mouse exon II, and, as expected, gave rise to a 238-bp amplified fragment using human genomic DNA or DNA from the parental human cell line NAIMR91 as a template (data not shown).…”

Section: Methodsmentioning

confidence: 78%

“…Primers for this experiment overlapped the region homologous to mouse exon II, and, as expected, gave rise to a 238-bp amplified fragment using human genomic DNA or DNA from the parental human cell line NAIMR91 as a template (data not shown). Analysis of the somatic-cell-hybrid panel 2 revealed that the agouti-specific 238-bp fragment was detected only in cell line NA10478 (data not shown), which is known to carry only human chromosome 20 in 84% of its cells and only chromosome 4 in another 8% of its cells (24). No specific products were amplified from the parental mouse line (NA05862), from the Chinese hamster cell line (NA10658), or from any of the other cell hybrids-particularly hybrid NAlOllS, which carries only human chromosome 4.…”

Section: Methodsmentioning

confidence: 99%

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Molecular structure and chromosomal mapping of the human homolog of the agouti gene.

Kwon

Bultman

Löffler

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

154

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The agouti (a) locus in mouse chromosome 2 normally regulates coat color pigmentation. The mouse agouti gene was recently cloned and shown to encode a distinctive 131-amino acid protein with a consensus signal peptide. Here we describe the cloning of the human homolog of the mouse agouti gene using an interspecies DNA-hybridization approach. Sequence analysis revealed that the coding region of the human agouti gene is 85% identical to the mouse gene and has the potential to encode a protein of 132 amino acids with a consensus signal peptide. Chromosomal assignment using somatic-cell-hybrid mapping panels and fluorescence in situ hybridization demonstrated that the human agouti gene maps to chromosome band 20q11.2. This result revealed that the human agouti gene is closely linked to several traits, including a locus called MODY (for maturity onset diabetes of the young) and another region that is associated with the development of myeloid leukemia. Initial expression studies with RNA from several adult human tissues showed that the human agouti gene is expressed in adipose tissue and testis.

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Section: Methodsmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Molecular structure and chromosomal mapping of the human homolog of the agouti gene.

Kwon

Bultman

Löffler

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

154

View full text Add to dashboard Cite

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“…Chromosomal Localization of the CaT-L Gene-The chromosomal localization of the human CaT-L gene was performed using NIGMS somatic hybrid mapping panel 2 (Coriell Institute, Camden, NJ) described previously (22,23) and primers corresponding to amino acids 115 YEGQTA and 158 NLIYFG of the CaT-L sequence (Fig. 3a).…”

Section: Construction Of Expression Plasmids and Transfection Of Hekmentioning

confidence: 99%

Expression of CaT-like, a Novel Calcium-selective Channel, Correlates with the Malignancy of Prostate Cancer

Wissenbach¹,

Niemeyer²,

Fixemer

et al. 2001

Journal of Biological Chemistry

261

232

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The regulation of intracellular Ca 2؉ plays a key role in the development and growth of cells. Here we report the cloning and functional expression of a highly calciumselective channel localized on the human chromosome 7. The sequence of the new channel is structurally related to the gene product of the CaT1 protein cloned from rat duodenum and is therefore called CaT-like (CaT-L). CaT-L is expressed in locally advanced prostate cancer, metastatic and androgen-insensitive prostatic lesions but is undetectable in healthy prostate tissue and benign prostatic hyperplasia. Additionally, CaT-L is expressed in normal placenta, exocrine pancreas, and salivary glands. New markers with well defined biological function that correlate with aberrant cell growth are needed for the molecular staging of cancer and to predict the clinical outcome. The human CaT-L channel represents a marker for prostate cancer progression and may serve as a target for therapeutic strategies.

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“…RP gene STSs were chromosomally assigned by use of National Institute of General Medical Sciences (NIGMS) human-rodent hybrid cell line panels 1 and 2 (Drwinga et al 1993).…”

Section: Physical Mappingmentioning

confidence: 99%

“…To assign each of these 75 rp genes to an individual human chromosome, we tested two panels of human-rodent hybrid cell line DNAs (Drwinga et al 1993) for the presence of the corresponding STS. For each of the rp gene STSs the chromosomal assignments derived by use of the first and second hybrid panels were concordant.…”

Section: Chromosomal Assignmentsmentioning

confidence: 99%

A Map of 75 Human Ribosomal Protein Genes

Kenmochi

Kawaguchi²,

Rozen³

et al. 1998

Genome Res.

146

105

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We mapped 75 genes that collectively encode >90% of the proteins found in human ribosomes. Because localization of ribosomal protein genes (rp genes) is complicated by the existence of processed pseudogenes, multiple strategies were devised to identify PCR-detectable sequence-tagged sites (STSs) at introns. In some cases we exploited specific, pre-existing information about the intron/exon structure of a given human rp gene or its homolog in another vertebrate. When such information was unavailable, selection of PCR primer pairs was guided by general insights gleaned from analysis of all mammalian rp genes whose intron/exon structures have been published. For many genes, PCR amplification of introns was facilitated by use of YAC pool DNAs rather than total human genomic DNA as templates. We then assigned the rp gene STSs to individual human chromosomes by typing human-rodent hybrid cell lines. The genes were placed more precisely on the physical map of the human genome by typing of radiation hybrids or screening YAC libraries. Fifty-one previously unmapped rp genes were localized, and 24 previously reported rp gene localizations were confirmed, refined, or corrected. Though functionally related and coordinately expressed, the 75 mapped genes are widely dispersed: Both sex chromosomes and at least 20 of the 22 autosomes carry one or more rp genes. Chromosome 19, known to have a high gene density, contains an unusually large number of rp genes (12). This map provides a foundation for the study of the possible roles of ribosomal protein deficiencies in chromosomal and Mendelian disorders.[The sequence data described in this paper have been submitted to GenBank. They are listed in Table 1.]Although the ribosome, as catalyst for protein synthesis, is known to be essential for organismal growth and development, the effects of ribosomal mutations and their role in human disease have been explored barely. The mammalian ribosome is a massive structure composed of 4 RNA species and ∼80 different proteins (Wool 1979). Typical mammalian cells contain about 4 ‫ן‬ 10 6 ribosomes, and ribosomal RNAs and proteins constitute ∼80% of all cellular RNA and 5%-10% of cellular protein. One might predict that genetic defects in ribosomal components would invariably result in early embryonic death. However, there is strong evidence in Drosophila and suggestive evidence in humans that quantitative deficiencies of ribosomal proteins may yield viable but abnormal phenotypes. In Drosophila, the Minute phenotype (reduced body size, diminished fertility, and short, thin bristles) results from heterozygous deficiencies (deletions) at any 1 of 50 loci scattered about the genome (Schultz 1929;FlyBase 1997). Several Minute loci have been characterized molecularly, and all have been found to encode ribosomal proteins (Kongsuwan et al. 1985;Hart et al. 1993;Andersson et al. 1994;Cramton and Laski 1994;Saebøe-Larssen and Lambertsson 1996;Schmidt et al. 1996; A. Cheng, A. Zinn, J. Mach, R. Lehman, and D.C. Page, unpubl.). Thus, it appears that redu...

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NIGMS Human/Rodent Somatic Cell Hybrid Mapping Panels 1 and 2

Cited by 134 publications

References 0 publications

Molecular structure and chromosomal mapping of the human homolog of the agouti gene.

Molecular structure and chromosomal mapping of the human homolog of the agouti gene.

Expression of CaT-like, a Novel Calcium-selective Channel, Correlates with the Malignancy of Prostate Cancer

A Map of 75 Human Ribosomal Protein Genes

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