“…Also, methods of analysis are different among the studies, with protocols based on scanning electron microscope (SEM) analysis, [20][21]27,[30][31]34,36,38 microhardness tests, 9,11,14,36,38,40 profilometric techniques, 4,15,18,34,36,[38][39] plasma-atomic emission spectrometric analysis associated with chromatography, 37 infrared absorption spectroscopy correlated with x-ray analysis, 41 atomic force microscopy 10,28 and nanoindentation techniques. 5 Conversely, only a few studies have attempted to assess whitening effects in vivo, 22,26,29 usually based on the analysis of an enamel replica using SEM. These studies on enamel surface characteristics 22,26,29 are mainly based on the morphologically subjective assessments of the enamel surface, rather than on precise measurements of the enamel surface profile.…”