Night-time transpiration in barley (<i>Hordeum vulgare</i>) facilitates respiratory carbon dioxide release and is regulated during salt stress

Even, Margaux; Sabo, Marine; Meng, Delong; Kreszies, Tino; Schreiber, Lukas; Fricke, Wieland

doi:10.1093/aob/mcy174

Cited by 8 publications

(13 citation statements)

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“…The rate of day-and night-time transpirational water loss was determined gravimetrically in the growth room using balances and computer software as described previously (Even et al 2018). Transpirational water loss was expressed either on a per-plant basis (m 3 s −1 ) or related to leaf surface area (m 3 m −2 s −1 or m s −1 ).…”

Section: Transpiration Measurementsmentioning

confidence: 99%

“…The number of stomata per unit projected leaf area was determined through a double-replica technique (Even et al 2018). Leaf two was analyzed halfway along the blade on the abaxial (lower) surface.…”

Section: Stomatal Densitymentioning

confidence: 99%

“…Leaf two was analyzed halfway along the blade on the abaxial (lower) surface. Previous work on the same barley cultivar has shown that stomatal density differs little between the adaxial and abaxial surface of leaf two (Even et al 2018, Armand et al 2019. Between 7 and 8 replicate plants were analyzed of each treatment, with three to five measurements taken per plant and leaf replica.…”

Section: Stomatal Densitymentioning

confidence: 99%

“…Residual transpiration was measured on detached leaves, as water loss over time (24 h), following the procedure described in Even et al (2018). The blade of the youngest fully expanded leaf of a plant was cut near the ligule.…”

Section: Residual Transpirationmentioning

confidence: 99%

“…Measurements were made initially at 20 min, then 30 min, then 2 h-intervals and finally over a period of several hours. The rate of initial water loss is thought to reflect water loss through stomata which are closed incompletely; the water loss which occurs subsequently is thought to reflect mainly water loss through the leaf cuticle (see also, Even et al 2018). Five plants were analyzed of each PAR treatment, and all plants and treatments were analyzed in parallel.…”

Section: Residual Transpirationmentioning

confidence: 99%

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Photosynthetically active radiation impacts significantly on root and cell hydraulics in barley (Hordeum vulgare L.)

Saini

Fricke

2020

Physiologia Plantarum

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Photosynthetically active radiation (PAR) affects transpirational water loss, yet we do not know through which mechanisms root water uptake is adjusted in parallel. Here, we exposed hydroponically grown barley plants to three levels of PAR [Normal (control), Low, High] and focused on the role which aquaporins (AQPs), apoplastic barriers (Casparian bands, suberin lamellae) and root morphology play in the adjustment of root hydraulic conductivity (Lp). Plants were analyzed when they were 14-18 days (d) old. Root and cell Lp, which involves AQP activity, was determined through exudation and cell pressure probe measurements, respectively. Gene expression of AQPs was analyzed through qPCR. The formation of apoplastic barriers was studied through staining of cross-sections. The rate of transpirational water loss per plant and unit leaf area increased in response to high-PAR and decreased in response to low-PAR treatments, both during day and night. Hydraulic conductivity in roots decreased significantly at organ and cell level in response to Low-PAR, and increased (organ) or did not change (cell level) in response to High-PAR. The formation of apoplastic barriers was little affected by PAR. Gene expression of AQPs tended to be highest in the Low-PAR treatment. Lateral roots, showing few apoplastic barriers, contributed the least in Low-and the most to root surface area in High-PAR plants. It is concluded that barley plants which experience changes in shoot transpirational water loss in response to PAR adjust root water uptake through changes in root Lp, and that these changes are mediated through altered AQP activity and root morphology.

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Section: Transpiration Measurementsmentioning

confidence: 99%

Section: Stomatal Densitymentioning

confidence: 99%

Section: Stomatal Densitymentioning

confidence: 99%

Section: Residual Transpirationmentioning

confidence: 99%

Section: Residual Transpirationmentioning

confidence: 99%

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Photosynthetically active radiation impacts significantly on root and cell hydraulics in barley (Hordeum vulgare L.)

Saini

Fricke

2020

Physiologia Plantarum

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Leaf physiological and morphological constraints of water-use efficiency in C3 plants

et al. 2023

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The increasing evaporative demand due to climate change will significantly affect the balance of carbon assimilation and water losses of plants worldwide. The development of crop varieties with improved water use efficiency (WUE) will be critical for adapting agricultural strategies under predicted future climate. This review aims to summarize the most important leaf morpho-physiological constraints of WUE in C3 plants and identify gaps in knowledge. From the carbon gain side of the WUE, the discussed parameters are mesophyll conductance, carboxylation efficiency and respiratory losses. The traits and parameters affecting the waterside of WUE balance discussed in this review are stomatal size and density, stomatal control and residual water losses (cuticular and bark conductance), nocturnal conductance and leaf hydraulic conductance. In addition, we discussed the impact of leaf anatomy and crown architecture on both the carbon gain and water loss components of WUE. There are multiple possible targets for future development in understanding sources of WUE variability in plants. We identified residual water losses and respiratory carbon losses as the greatest knowledge gaps of whole plant WUE assessments. Moreover, the impact of trichomes, leaf hydraulic conductance and canopy structure on plants’ WUE is still not well understood. The development of a multi-trait approach is urgently needed for a better understanding of WUE dynamics and optimization.

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Diversity in daytime and night‐time transpiration dynamics in barley indicates adaptation to drought regimes across the Middle‐East

Sadok

Tamang

2019

J Agronomy Crop Science

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A challenge to breeding drought‐tolerant barley in the Middle‐East is that precipitation and evaporative demand patterns dictate opposite water use strategies (conservative vs. risk‐taking). To characterize these strategies, we examined high‐resolution, whole‐plant transpiration rate (TR) responses to increasing vapour pressure deficit (VPD) and nocturnal TR (TRN) dynamics among 25 local barley genotypes, using a novel phenotyping system. These traits were specifically selected because they exist under modalities enabling the expression of both strategies. The genotypes were selected from locations spread across a large aridity gradient represented by temperature and precipitation data spanning 30 years. Here, we uncovered a substantial diversity in TR responses to VPD where slopes of the linear responses correlated negatively with local maximal temperatures, pointing to opposite drought tolerance strategies. Low canopy conductance (low slopes) was associated with higher aridity, likely to enable water‐saving, while higher conductance was associated with wetter areas, likely to enable a more aggressive water use to maximize physiological activity. TRN was highly diverse and represented up to 15% of maximal daytime TR, pointing to the possibility of increasing water‐saving by reducing TRN. Furthermore, we detected pre‐dawn variation in TRN that negatively correlated with local precipitation, indicating that a tighter circadian control is associated with adaptation to drought, consistently with a circadian resonance mechanism. These findings indicate that canopy conductance and TRN are potentially beneficial to design drought‐tolerant barley germplasm adapted to different drought regimes taking place in the Middle‐East.

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Night-time transpiration in barley (Hordeum vulgare) facilitates respiratory carbon dioxide release and is regulated during salt stress

Cited by 8 publications

References 0 publications

Photosynthetically active radiation impacts significantly on root and cell hydraulics in barley (Hordeum vulgare L.)

Photosynthetically active radiation impacts significantly on root and cell hydraulics in barley (Hordeum vulgare L.)

Leaf physiological and morphological constraints of water-use efficiency in C3 plants

Diversity in daytime and night‐time transpiration dynamics in barley indicates adaptation to drought regimes across the Middle‐East

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