Nigerian rotavirus serotype G8 could not be typedby PCR due to nucleotide mutation at the 3′ endof the primer binding site

Adah, Mohammed I.; Rohwedder, Angela; Olaleyle, O. D.; Werchau, H

doi:10.1007/s007050050206

Cited by 80 publications

(91 citation statements)

References 18 publications

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“…The result of enzyme assays showed elevation of both ALP and AST in all the groups of infected rats seen in table 2. The elevaton of these enzymes is in consonance with earlier reports by Gray (1986) and Adah et al, (1997). This has been reported to due to inflammation and necrosis in the infected host particularly in organs like liver, kidney muscle and even heart (Losos and Ikede, 1972).…”

Section: Discussionsupporting

confidence: 90%

Crude methanolic extract of <em>Moringa oleifera</em> leaves improves the efficacy of diminazene aceturate in the treatment of trypanosome infected rats

et al. 2017

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A trypanocidal efficacy study of Moringa oleifera leaf supplement alone and in combination with diminazene aceturate on Wistar rats was conducted using Trypanosoma brucei. Thirty five rats were randomly allotted into seven groups (A-G) with include five rats each. Groups B and C were treated with methanolic extract of M. oleifera leaves at 200mg/kg for 14 days prior to infection. The E and F were treated with M. oleifera for 90 days at 200mg/kg prior to infection. Group A and G are negative and positive controls, respectively. All rats in groups, B -G were individually infected with 3x10 6 of Trypanosoma brucei per ml of blood and the prepatent period was monitored from the second day. At day five post infection, infected rats in group C, D and F were treated with diminazene aceturate at 7mg/kg while those in group B and E treated with M. oleifera leaf extract. Parasites were cleared within 72 hours post treatment from blood of rats in groups C and F which were treated with both M. aoleifera and diminazine aceturate. However, it took 96 hours post treatment for parasites to be cleared from rats in group D which was treated only with diminazene aceturate. There were significant (P<0.05) increase in the erythrocyte count, haematocrit and mean corpuscular volume (MCV) in M. oleifera treated groups. Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Haemoglobin Concentration (MCHC) decreased significantly (P<0.05) in groups E, F and G. There was neutropenia in groups E, F and G when compared with the negative control, while other leucocytes manifested leukocytosis. Serum chemistry showed that all groups had significantly increased (P<0.05) globulin, blood urea nitrogen (BUN) and liver enzymes when compared with the negative control group "A". Histopathological findings showed that congestion in the spleen and lymph nodes were reduced in the treated groups when compared to the untreated groups. Conclusively, our study gives credence that M. oleifera does not possess trypanocidal effect but however could be coadministered as a supportive.

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Section: Discussionsupporting

confidence: 90%

Crude methanolic extract of <em>Moringa oleifera</em> leaves improves the efficacy of diminazene aceturate in the treatment of trypanosome infected rats

et al. 2017

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“…Sequence analysis, shows that there are point mutations at the P-type-specifi c primer binding sites which may caused genotyping failure. The mutation in the primer binding site that cause nucleotide mismatches between the VP4 gene and primer sequence also reported by Adah et al (1997), Iturriza-Gomara et al (2000), and Iturriza-Gomara et al (2004). Figure 2 and 3 described the location of primer that were used in this study.…”

Section: Resultssupporting

confidence: 60%

Genotyping of Rotavirus by Using RT-PCR Methods

et al. 2015

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There is a great diversity of rotavirus genotypes circulating worldwide, with dominant genotypes changing from year to year. Rotavirus genotyping was performed by using reverse transcription PCR with type-specifi c-primers. Since rotavirus is a RNA virus that has high mutation rate, there was a possibility of technical diffi culty in genotyping due to mutation in the primer binding sites. During Indonesian rotavirus surveillance study 2006-2009, it was reported that 17% of samples subjected for G type and 21% of samples subjected for P type were untypeable. The objective of this study was to identify genotypes of the samples that were untypeable previously using RT-PCR based on the method described by Das et al. (1994) and Gentsch et al. (1992). There were 30 samples subjected to G type and 61 samples subjected to P type to be re-typed using method described by Gouvea et al. (1990) and Simmond et al. (2008) for G and P typing, respectively. By using another set of primer, the genotype of all samples was identifi ed. This study highlights the importance of a constant reconsideration of primer sequences employed for the molecular typing of rotaviruses.

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“…Therefore, failure to type is most likely to be associated with technical issues and/or low viral load, although occasionally they may represent usual genotypes, particularly in those associated with an animal rotavirus G-or P-types [21][22][23][24][25][26]. The failure to identify G-types associated with P [6], P [9], P [10], P [11] and P [14] may be associated with the presence of unusual G-types for which G genotype-specific primers were not included in the study protocol.…”

Section: Distribution According To Gendermentioning

confidence: 99%

Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network

Iturriza‐Gómara¹,

Dallman²,

Bányai³

et al. 2010

Epidemiol. Infect.

205

254

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SUMMARYEuroRotaNet, a laboratory network, was established in order to determine the diversity of co-circulating rotavirus strains in Europe over three or more rotavirus seasons from 2006/2007 and currently includes 16 countries. This report highlights the tremendous diversity of rotavirus strains co-circulating in the European population during three years of surveillance since 2006/ 2007 and points to the possible origins of these strains including genetic reassortment and interspecies transmission. Furthermore, the ability of the network to identify strains circulating with an incidence of o1% allowed the identification of possible emerging strains such as G8 and G12 since the beginning of the study ; analysis of recent data indicates their increased incidence.

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Nigerian rotavirus serotype G8 could not be typedby PCR due to nucleotide mutation at the 3′ endof the primer binding site

Cited by 80 publications

References 18 publications

Crude methanolic extract of <em>Moringa oleifera</em> leaves improves the efficacy of diminazene aceturate in the treatment of trypanosome infected rats

Crude methanolic extract of <em>Moringa oleifera</em> leaves improves the efficacy of diminazene aceturate in the treatment of trypanosome infected rats

Genotyping of Rotavirus by Using RT-PCR Methods

Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network

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