Nigella sativa Oil Alleviates Mouse Testis and Sperm Abnormalities Induced by BPA Potentially through Redox Homeostasis

Al-Griw, Mohamed A.; Shalab, Suliman M; Alghazeer, Rabia; Elnfat, Abdul Hakim; Treesh, Soad A.; Benjama, Ahmeda; Shamlan, Ghalia; Habibullah, Mahmmoud M; Eskandrani, Areej A.; Alnajeebi, Afnan M.; Babteen, Nouf Abubakr; Alansari, Wafa S.

doi:10.2174/1386207325666220514135606

Cited by 3 publications

(3 citation statements)

References 65 publications

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“…BPA can increase the ROS and lipid peroxidation in testicular cells, and decrease the content of GSH as well as the expression of antioxidant enzymes SOD and CAT, leading to testicular cell apoptosis ( Nguyen et al, 2022 ; Al-Griw et al, 2023 ). Taurine has been used to alleviate oxidative stress in mouse testicular cells ( Rezaee-Tazangi et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Melatonin alleviates oxidative stress damage in mouse testes induced by bisphenol A

Qi,

Yang,

et al. 2024

Front. Cell Dev. Biol.

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We investigated the effect of melatonin on bisphenol A (BPA)-induced oxidative stress damage in testicular tissue and Leydig cells. Mice were gavaged with 50 mg/kg BPA for 30 days, and concurrently, were injected with melatonin (10 mg/kg and 20 mg/kg). Leydig cells were treated with 10 μmol/L of BPA and melatonin. The morphology and organ index of the testis and epididymis were observed and calculated. The sperm viability and density were determined. The expressions of melatonin receptor 1A and luteinizing hormone receptor, and the levels of malonaldehyde, antioxidant enzymes, glutathione, steroid hormone synthases, aromatase, luteinizing hormone, testosterone, and estradiol were measured. TUNEL assay was utilized to detect testicular cell apoptosis. The administration of melatonin at 20 mg/kg significantly improved the testicular index and epididymis index in mice treated with BPA. Additionally, melatonin promoted the development of seminiferous tubules in the testes. Furthermore, the treatment with 20 mg/kg melatonin significantly increased sperm viability and sperm density in mice, while also promoting the expressions of melatonin receptor 1A and luteinizing hormone receptor in Leydig cells of BPA-treated mice. Significantly, melatonin reduced the level of malonaldehyde in testicular tissue and increased the expression of antioxidant enzymes (superoxide dismutase 1, superoxide dismutase 2, and catalase) as well as the content of glutathione. Moreover, melatonin also reduced the number of apoptotic Leydig cells and spermatogonia, aromatase expression, and estradiol level, while increasing the expression of steroid hormone synthases (steroidogenic acute regulatory protein, cytochrome P450 family 17a1, cytochrome P450 17α-hydroxylase/20-lyase, and, 17β-hydroxysteroid dehydrogenase) and the level of testosterone. Melatonin exhibited significant potential in alleviating testicular oxidative stress damage caused by BPA. These beneficial effects may be attributed to melatonin’s ability to enhance the antioxidant capacity of testicular tissue, promote testosterone synthesis, and reduce testicular cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Melatonin alleviates oxidative stress damage in mouse testes induced by bisphenol A

Qi,

Yang,

et al. 2024

Front. Cell Dev. Biol.

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“…Despite the use of modern therapies to support sexual desire and relieve sexual dysfunction, natural alternative medicine has earned substantial consideration among scientists due to its wide safety margin and low price [ 5 ]. Among those natural medicines are botanical sources, such as Nigella sativa [ 6 ] and honey [ 7 ]. Nigella sativa Linn., a member of the Ranunculaceae family and commonly referred to as “black seed”, or Habbatul Barakah, has long been utilized in the Middle East, Northern Africa, and Asia as a traditional medicine for digestive and respiratory disorders and boosts the immune system.…”

Section: Introductionmentioning

confidence: 99%

“…Pharmacologically, the seeds of Nigella sativa have antioxidant, anti-inflammatory, antiviral, antibacterial, and antidiabetic effects [ 10 , 11 ]. Moreover, Nigella sativa oil (NS oil) has a favorable impact on testicular protective properties, particularly, semen characteristics and testosterone [ 6 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Nigella sativa-Floral Honey and Multi-Floral Honey versus Nigella sativa Oil against Testicular Degeneration Rat Model: The Possible Protective Mechanisms

et al. 2023

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The male reproductive function, particularly the testes, and the related hormones are sensitive to various xenobiotics. This work aimed for the first time to assess Nigella sativa floral honey (NS floral honey) and multi-floral honey (M-floral honey) versus Nigella sativa oil (NS oil) against rat testicular degeneration induced with azathioprine (AZA). A total of 40 male Wister rats were assigned into 5 groups: (1) control, (2) 15 mg/kg of AZA, (3) AZA + 1.4 mL/kg of M-floral honey, (4) AZA + 1.4 mL/kg of NS floral honey, and (5) AZA + 500 mg/kg of NA oil. Total testosterone (TT), free testosterone (FT), free androgen index (FAI), gonadotrophins, sex-hormone-binding globulin (SHBG), apoptosis markers, and redox status were assessed to clarify the possible protective mechanisms. Pituitary–testicular axis disruption, apoptosis markers, poor redox status, and sperm quality (count, viability, and motility) were set with AZA. Serum TT, SHBG, and absolute and relative testis weight were significantly restored in the NS oil and NS floral honey groups. Meanwhile, the NS oil group exhibited a significant elevation in FT and FAI. Serum gonadotrophins increased significantly in the NS floral honey (p < 0.01) and M-floral honey and NS oil (p < 0.05) groups. Testicular caspase-3, caspase-9, and nitric oxide showed significant improvement in the NS floral honey and NS oil groups. NS oil supplementation significantly normalized redox status (p < 0.05), whereas NS floral honey improved malondialdehyde and superoxide dismutase activity. Sperm quality exhibited a significant improvement in the NS oil group (p < 0.05). M-floral honey did not show reliable results. Although NS floral honey could protect against testicular damage, it did not upgrade to the level of improvement achieved with NS oil. We claim that further clinical studies are essential for focusing on the quality and quantity of bioactive constituents.

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Vitamin D ameliorates liver pathology in mice caused by exposure to endocrine disruptor bisphenol A

Griw¹,

Zaed²,

Hdud³

et al. 2023

Open Vet J

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Background: Increasing evidence suggests that bisphenol A (BPA) induces liver pathological changes. Further, an association between BPA and circulating vitamin D (VitD) levels were documented. Aim: The role of VitD in BPA-induced liver pathological changes was explored in this study. Methods: Healthy 4.5-week-old male (n=35) and female (n=35) Swiss albino mice were used in this study. The animals were randomly divided into control and treated groups. The control groups were further divided into sham (no treatment) and vehicle (corn oil), whereas the treated groups were also divided into VitD (2195U/kg), BPA (50μg/kg), and BPA+VitD (50μg/kg+2195U/kg) groups. For six weeks (twice a week), the animals were dosed intraperitoneally (IP). One week later (at 10.5-weeks-old), the animals were sacrificed for biochemical and histological analyses. Results: BPA produced a considerable rise in the body and liver weights in both genders of mice when compared to control mice. BPA also caused significant increases in the liver damage markers alanine transaminase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT). It also induced liver histopathological changes, including higher apoptotic indices in both genders. On the other hand, treatment with VitD considerably reduced liver damage and slightly decreased the apoptotic index rate. The ALP, ALT, and GGT levels were also markedly reduced. VitD has been proven to have a protective effect on both genders. Conclusions: According to our findings, VitD protects mice from BPA-induced liver damage, possibly via suppressing liver damage markers.

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Nigella sativa Oil Alleviates Mouse Testis and Sperm Abnormalities Induced by BPA Potentially through Redox Homeostasis

Cited by 3 publications

References 65 publications

Melatonin alleviates oxidative stress damage in mouse testes induced by bisphenol A

Melatonin alleviates oxidative stress damage in mouse testes induced by bisphenol A

Nigella sativa-Floral Honey and Multi-Floral Honey versus Nigella sativa Oil against Testicular Degeneration Rat Model: The Possible Protective Mechanisms

Vitamin D ameliorates liver pathology in mice caused by exposure to endocrine disruptor bisphenol A

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