Nigella A ameliorates inflammation and intestinal flora imbalance in DSS induced colitis mice

Abstract

Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. However, whether Nigella A could treat ulcerative colitis (UC) is still unknown. As saponins always be regarded as the kinds of ingredients that could regulate immunity and intestinal flora. This research aimed to investigate the therapeutic effect of Nigella A on UC and explore its effect on intestinal flora. We noted that Nigella A and Sulfasalazine (SASP) could significantly improve the signs and symptoms, alleviate colonic pathological injury in DSS-induced mice. The changing of many specific bacterial genus such as Lactobacillus, Porphyromonadaceae, Bacteroides and Escherichia might closely related to the recovery of intestinal inflammatory response. This study initially confirmed the therapeutic effect of Nigella A and SASP on DSS-induced colitis by improving the diversity of intestinal microbial composition. Nigella A has the potential to be developed for the treatment of UC and other disorders related to the imbalance of intestinal flora.

Desmethylbellidifolin Attenuates Dextran Sulfate Sodium-Induced Colitis: Impact on Intestinal Barrier, Intestinal Inflammation and Gut Microbiota

“…We have found a striking difference of Bacteroides in DSS-treated mice ( Fig. 6 b ), which was similar with the observations reported by Okayasu et al and Hu et al 32 , 33 . Moreover, it has been reported that mice transplanted with Bacteroides fragilis could develop persistent colitis 34 .…”

Hydroxytyrosol Alleviates Dextran Sulfate Sodium-Induced Colitis by Modulating Inflammatory Responses, Intestinal Barrier, and Microbiome

“…As shown in Figure a, an obvious difference in the microbial diversity was observed among the NC, DSS, and HT-treated groups ( p < 0.05). The Shannon index (community diversity) of the DSS treatment group mice decreased by 20.19% compared with the NC group ( p < 0.05), confirming that DSS induced the decrease in the intestinal flora diversity in mice . The abatement of the gut microbial diversity in HT-L- and HT-H-treated groups was improved by 4.60 and 13.10%, respectively.…”

“…The Shannon index (community diversity) of the DSS treatment group mice decreased by 20.19% compared with the NC group (p < 0.05), confirming that DSS induced the decrease in the intestinal flora diversity in mice. 40 The abatement of the gut microbial diversity in HT-L-and HT-H-treated groups was improved by 4.60 and 13.10%, respectively. A similar trend was found in β-diversity, where the DSS-treated group caused significant changes in the diversity of the gut microbiota but the high-dose HT treatment greatly reversed this transformation by shifting it back toward the NC group (Figure 6b).…”

Taenia solium cysticerci's extracellular vesicles Attenuate the AKT/mTORC1 pathway for Alleviating DSS‐induced colitis in a murine model

“…Intriguingly, we did not see any weight gain in the EV‐stimulated groups, and it is possible that a longer stimulation duration of EV may be required in mice to see recovery in weight. Apart from that, therapeutic investigation of different molecules showed improved histopathology scores without any weight gain indicating weight gain is not always associated with improved disease severity and can be explored further (Hu et al., 2020 ; Xue et al., 2023 ). Colon lengths in the EV groups were longer than the DSS‐only group.…”