Nidogen-2: Location and expression during corneal wound healing

Gallego‐Muñoz, Patricia; Lorenzo-Martín, Elvira; Fernández, Itziar; Herrero-Pérez, Cristina; Martínez‐García, Carmen

doi:10.1016/j.exer.2018.09.004

Cited by 5 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA-seq analysis revealed significant differences in the transcription levels of 16 genes involved in extracellular matrix and proteinaceous extracellular terms. The NIH/3T3 cells exposed to the control-derived metabolites treated group exhibited a significant increase in cysteine-rich secretory protein LCCL domain containing 2 ( Crispld2 ), dermatopontin ( Dpt ), SPARC-related modular calcium binding 2 ( Smoc2 ), lumican ( Lum ), nidogen 2 ( Nid ), laminin gamma 3 ( Lamc3 ), thrombospondin domain containing 4 ( Thsd4 ), collagen type XXVIII alpha 1 chain ( Col28a1 ), ADAM metallopeptidase with thrombospondin Type 1 motif 15 ( Adamts15 ), and retinoic acid responder 2 ( Rarres2 ), and these genes were found to have a positive association with fibrosis. − In the control treatment group, there was a notable reduction in the transcript levels of leucine rich repeat protein 3 ( Lrrn 3), which was believed to have a reverse correlation with fibrosis (Figure B) . In contrast, NIH/3T3 cells treated with inulin-derived metabolites exhibited a significant increase in wingless-type MMTV integration site family 5b ( Wnt5b ) and Von Willebrand factor ( Vwf ), both recognized for their positive correlation with fibrosis (Figure B). , The quantification of these genes via qPCR corresponded with the results of RNA sequencing (Figure C).…”

Section: Resultsmentioning

confidence: 99%

Exploring the Role of Inulin in Targeting the Gut Microbiota: An Innovative Strategy for Alleviating Colonic Fibrosis Induced By Irradiation

Ji,

Zhang,

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

The use of radiation therapy to treat pelvic and abdominal cancers can lead to the development of either acute or chronic radiation enteropathy. Radiation-induced chronic colonic fibrosis is a common gastrointestinal disorder resulting from the above radiation therapy. In this study, we establish the efficacy of inulin supplements in safeguarding against colonic fibrosis caused by irradiation therapy. Studies have demonstrated that inulin supplements enhance the proliferation of bacteria responsible to produce short-chain fatty acids (SCFAs) and elevate the levels of SCFAs in feces. In a mouse model of chronic radiation enteropathy, the transplantation of gut microbiota and its metabolites from feces of inulin-treated mice were found to reduce colonic fibrosis in validation experiments. Administering inulin-derived metabolites from gut microbiota led to a notable decrease in the expression of genes linked to fibrosis and collagen production in mouse embryonic fibroblast cell line NIH/3T3. In the cell line, inulin-derived metabolites also suppressed the expression of genes linked to the extracellular matrix synthesis pathway. The results indicate a novel and practical approach to safeguarding against chronic radiation-induced colonic fibrosis.

show abstract

Section: Resultsmentioning

confidence: 99%

Exploring the Role of Inulin in Targeting the Gut Microbiota: An Innovative Strategy for Alleviating Colonic Fibrosis Induced By Irradiation

Ji,

Zhang,

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Formation of this basement membrane may play an important role in protecting the stroma from pro-fibrotic factors that are secreted by the epithelium following injury [2,3]. Epithelial cells are considered the dominant source for assembling the epithelial basement membrane with possible contributions from stromal keratocytes [39,40]. To determine if the epithelium begins to express basement membrane proteins by 1 week when cultured in our co-culture system, we stained for laminin-1β, a highly abundant protein found in the epithelial basement membrane [38].…”

Section: Resultsmentioning

confidence: 99%

Corneal Epithelial–Stromal Fibroblast Constructs to Study Cell–Cell Communication in Vitro

et al. 2019

View full text Add to dashboard Cite

Cell–cell communication plays a fundamental role in mediating corneal wound healing following injury or infection. Depending on the severity of the wound, regeneration of the cornea and the propensity for scar development are influenced by the acute resolution of the pro-fibrotic response mediated by closure of the wound via cellular and tissue contraction. Damage of the corneal epithelium, basement membrane, and anterior stroma following a superficial keratectomy is known to lead to significant provisional matrix deposition, including secretion of fibronectin and thrombospondin-1, as well as development of a corneal scar. In addition, corneal wounding has previously been shown to promote release of extracellular vesicles from the corneal epithelium, which, in addition to soluble factors, may play a role in promoting tissue regeneration. In this study, we report the development and characterization of a co-culture system of human corneal epithelial cells and corneal stromal fibroblasts cultured for 4 weeks to allow extracellular matrix deposition and tissue maturation. The secretion of provisional matrix components, as well as small and large extracellular vesicles, was apparent within the constructs, suggesting cell–cell communication between epithelial and stromal cell populations. Laminin-1β was highly expressed by the corneal epithelial layer with the presence of notable patches of basement membrane identified by transmission electron microscopy. Interestingly, we identified expression of collagen type III, fibronectin, and thrombospondin-1 along the epithelial–stromal interface similar to observations seen in vivo following a keratectomy, as well as expression of the myofibroblast marker, α-smooth muscle actin, within the stroma. Our results suggest that this corneal epithelial–stromal model may be useful in the study of the biochemical phenomena that occur during corneal wound healing.

show abstract

“…Throughout the healing process, activated stromal cells also secreted nidogen-2. The stromal contribution may, however, be inadequate to revitalize BM structure and function in severe alkaline wounds [ 79 ].…”

Section: Nidogenmentioning

confidence: 99%

Wound healing of the corneal epithelium: a review

2021

View full text Add to dashboard Cite

The corneal epithelium (CE) forms the outermost layer of the cornea. Despite its thickness of only 50 μm, the CE plays a key role as an initial barrier against any insults to the eye and contributes to the light refraction onto the retina required for clear vision. In the event of an injury, the cornea is equipped with many strategies contributing to competent wound healing, including angiogenic and immune privileges, and mechanotransduction. Various factors, including growth factors, keratin, cytokines, integrins, crystallins, basement membrane, and gap junction proteins are involved in CE wound healing and serve as markers in the healing process. Studies of CE wound healing are advancing rapidly in tandem with the rise of corneal bioengineering, which employs limbal epithelial stem cells as the primary source of cells utilizing various types of biomaterials as substrates.

show abstract

Nidogen-2: Location and expression during corneal wound healing

Cited by 5 publications

References 37 publications

Exploring the Role of Inulin in Targeting the Gut Microbiota: An Innovative Strategy for Alleviating Colonic Fibrosis Induced By Irradiation

Exploring the Role of Inulin in Targeting the Gut Microbiota: An Innovative Strategy for Alleviating Colonic Fibrosis Induced By Irradiation

Corneal Epithelial–Stromal Fibroblast Constructs to Study Cell–Cell Communication in Vitro

Wound healing of the corneal epithelium: a review

Contact Info

Product

Resources

About