Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells

Qian, Jie; Mummalaneni, Shobha; Larsen, James D; Grider, John R.; Spielman, Andrew I.; Özdener, Mehmet Hakan; Lyall, Vijay

doi:10.1371/journal.pone.0194089

Cited by 6 publications

(6 citation statements)

References 54 publications

(102 reference statements)

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“…3.3.4) ( 170 , 171 , 349 , 350 ). Again, using HBO cells, Ozdener and colleagues ( 351 ) showed that T2R-expressing cells express nicotinic receptors and respond directly to nicotine and acetylcholine, producing transient elevations in intracellular calcium. The significance of this opus is that it may provide a mechanism for the taste disturbances that are a side effect of certain anticholinergic drugs.…”

Section: Research In Progressmentioning

confidence: 99%

Physiology of the tongue with emphasis on taste transduction

Doyle

Premathilake

Yao

et al. 2023

Physiological Reviews

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The tongue is a complex multifunctional organ that interacts and senses both interoceptively and exteroceptively. Although it is easily visible to almost all of us it is relatively understudied and what is in the literature as regards taste mechanisms is often contradictory or is not comprehensively reported. The tongue is both a motor and a sensory organ: motor in that it is required for speech and mastication, and sensory in that it receives information to be relayed to the central nervous system pertaining to the safety and quality of the contents of the oral cavity. Additionally, the tongue and its taste apparatus form part of an innate immune surveillance system. For example, loss or alteration in taste perception can be an early indication of infection as became evident during the present global SARS-CoV-2 pandemic. Here we particularly emphasize the latest updates in the mechanisms of taste perception, taste bud formation and adult taste bud renewal, the presence, and effects of hormones on taste perception, review the understudied lingual immune system with specific reference to SARS-CoV-2, discuss nascent work on tongue microbiome, as well as addressing the effect of systemic disease on tongue structure and function, especially in relation to taste.

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Section: Research In Progressmentioning

confidence: 99%

Physiology of the tongue with emphasis on taste transduction

Doyle

Premathilake

Yao

et al. 2023

Physiological Reviews

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“…HBO cells were derived from two male and two female volunteers and were developed in Dr. Ozdener's lab at Monell Chemical Senses Center, as a model of human TRCs in vivo. HBO cells stably display all molecular and physiological features characteristic of mature taste cells and exhibit an increase in intracellular calcium ([Ca 2+ ] i ) in response to taste stimuli representing all five taste qualities, indicating the presence of all known signaling pathways [5,[21][22][23][24][25]. HBO cells were cultured as described earlier [5,21,22] and were used between passages 4 and 8.…”

Section: Hbo Cell Culturementioning

confidence: 99%

“…HBO cells stably display all molecular and physiological features characteristic of mature taste cells and exhibit an increase in intracellular calcium ([Ca 2+ ] i ) in response to taste stimuli representing all five taste qualities, indicating the presence of all known signaling pathways [5,[21][22][23][24][25]. HBO cells were cultured as described earlier [5,21,22] and were used between passages 4 and 8. HBO cells express all four ENaC subunits [5,23].…”

Section: Hbo Cell Culturementioning

confidence: 99%

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Relationship between ENaC Regulators and SARS-CoV-2 Virus Receptor (ACE2) Expression in Cultured Adult Human Fungiform (HBO) Taste Cells

et al. 2022

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In addition to the α, β, and γ subunits of ENaC, human salt-sensing taste receptor cells (TRCs) also express the δ-subunit. At present, it is not clear if the expression and function of the ENaC δ-subunit in human salt-sensing TRCs is also modulated by the ENaC regulatory hormones and intracellular signaling effectors known to modulate salt responses in rodent TRCs. Here, we used molecular techniques to demonstrate that the G-protein-coupled estrogen receptor (GPER1), the transient receptor potential cation channel subfamily V member 1 (TRPV1), and components of the renin-angiotensin-aldosterone system (RAAS) are expressed in δ-ENaC-positive cultured adult human fungiform (HBO) taste cells. Our results suggest that RAAS components function in a complex with ENaC and TRPV1 to modulate salt sensing and thus salt intake in humans. Early, but often prolonged, symptoms of COVID-19 infection are the loss of taste, smell, and chemesthesis. The SARS-CoV-2 spike protein contains two subunits, S1 and S2. S1 contains a receptor-binding domain, which is responsible for recognizing and binding to the ACE2 receptor, a component of RAAS. Our results show that the binding of a mutated S1 protein to ACE2 decreases ACE2 expression in HBO cells. We hypothesize that changes in ACE2 receptor expression can alter the balance between the two major RAAS pathways, ACE1/Ang II/AT1R and ACE2/Ang-(1–7)/MASR1, leading to changes in ENaC expression and responses to NaCl in salt-sensing human fungiform taste cells.

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“…A similar mechanism may also be involved in the aversive responses to ethanol at high concentrations [ 92 ]. In support of this mechanism, both in rodent and human taste receptor cells, nAChRs are expressed in TRPM5 positive cells [ 93 , 94 ]. These studies suggest that both TRPM5-dependent and TRPM5-independent but nAChR-dependent pathways are present in bitter taste cells.…”

Section: Potential Role Of Trpa1 and Trpv1 As A Bridge Between Tasmentioning

confidence: 99%

Interactions between Chemesthesis and Taste: Role of TRPA1 and TRPV1

Rhyu

Kim

Lyall

2021

IJMS

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In addition to the sense of taste and olfaction, chemesthesis, the sensation of irritation, pungency, cooling, warmth, or burning elicited by spices and herbs, plays a central role in food consumption. Many plant-derived molecules demonstrate their chemesthetic properties via the opening of transient receptor potential ankyrin 1 (TRPA1) and transient receptor potential vanilloid 1 (TRPV1) channels. TRPA1 and TRPV1 are structurally related thermosensitive cation channels and are often co-expressed in sensory nerve endings. TRPA1 and TRPV1 can also indirectly influence some, but not all, primary taste qualities via the release of substance P and calcitonin gene-related peptide (CGRP) from trigeminal neurons and their subsequent effects on CGRP receptor expressed in Type III taste receptor cells. Here, we will review the effect of some chemesthetic agonists of TRPA1 and TRPV1 and their influence on bitter, sour, and salt taste qualities.

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Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells

Cited by 6 publications

References 54 publications

Physiology of the tongue with emphasis on taste transduction

Physiology of the tongue with emphasis on taste transduction

Relationship between ENaC Regulators and SARS-CoV-2 Virus Receptor (ACE2) Expression in Cultured Adult Human Fungiform (HBO) Taste Cells

Interactions between Chemesthesis and Taste: Role of TRPA1 and TRPV1

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