Nicotine regulates autophagy of human periodontal ligament cells through α7 nAchR that promotes secretion of inflammatory factors IL-1β and IL-8

Du, Yang; Yang, Kuan; Zhou, Zhifei; Wu, Li-An; Wang, Lulu; Chen, Yujiang; Wang, Xiaojing

doi:10.1186/s12903-021-01894-5

Cited by 9 publications

(7 citation statements)

References 54 publications

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“…Studies have shown that nicotine may inhibit the production of inflammatory factors in oral epithelial cells, 35 while other studies have shown that nicotine can also be produce cytotoxicity to human periodontal cells and induce human periodontal cells to produce IL-1β and IL-8. 36 E-cigarettes are cytotoxic to a variety of cells, including gingival fibroblasts, gingival mesenchymal stem cells, and gingival epithelial cells, and affect cell proliferation, but e-cigarettes have less effect on cell proliferation and viability than tobacco. 37 Therefore, more in-depth research is needed to explore the safety of e-cigarettes and how they compare to tobacco.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Liu

Yue

et al. 2023

ACS Omega

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Smoking increases the risk of a number of diseases, including cardiovascular, oral, and lung diseases. E-cigarettes are gaining popularity among young people as an alternative to cigarettes, but there is debate over whether they are less harmful to the mouth than e-cigarettes. In this study, human gingival epithelial cells (HGECs) were treated with four commercially available e-cigarette aerosol condensates (ECAC) or commercially available generic cigarette smoke condensates (CSC) with different nicotine concentrations. Cell viability was determined by MTT assay. Cell apoptosis was observed by acridine orange (AO) and Hoechst33258 staining. The levels of type I collagen, matrix metalloproteinase (MMP-1, MMP-3), cyclooxygenase 2, and inflammatory factors were detected by ELISA and RT-PCR. Finally, ROS levels were analyzed by ROS staining. The different effects of CSC and ECAC on HGECs were compared. The results showed that higher nicotine concentration of CS significantly reduced the activity of HGECs. By contrast, all ECAC had no significant effect. The levels of matrix metalloproteinase, COX-2, and inflammatory factors were higher in HGECs treated with CSC than those treated with ECAC. In contrast, the level of type I collagen was higher in HGECs treated with ECAC than those treated with CSC. In conclusion, all four flavors of e-cigarettes were less toxic to HGE cells than tobacco, but further clinical studies are needed to determine whether e-cigarettes are less harmful to oral health than conventional cigarettes.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Liu

Yue

et al. 2023

ACS Omega

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“…A nicotina, presente nos cigarros convencionais e eletrônicos, aumentou significativamente a autofagia das células do ligamento periodontal e estudos in vitro também mostram que o vaping tem um efeito prejudicial semelhante ao tabagismo na saúde do periodonto 8,28 .…”

Section: Discussionunclassified

“…Nessa perspectiva, a nicotina aumentou significativamente a autofagia das células do ligamento periodontal e a secreção dos fatores através do receptor nicotínico de acetilcolina, agravando, assim, a resposta inflamatória dos tecidos periodontais e, consequentemente, da periodontite. Portanto, os aerossóis dos cigarros eletrônicos são menos prejudiciais para os estreptococos comensais orais em comparação com a fumaça do cigarro convencional, mas os aromatizantes apresentam um efeito bactericida, matando os estreptococos, mas mantendo sua morfologia intacta, resultando em um prejuízo para o restante do micro bioma oral 28,29 .…”

Section: Discussionunclassified

Considerações atuais sobre o uso dos cigarros eletrônicos relacionados às doenças periodontais: revisão da literatura

Martins¹,

Neto²,

Freitas³

et al. 2023

Braz. J. Hea. Rev.

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As doenças periodontais, gengivite e periodontite, são multifatoriais e muitas vezes imperceptíveis aos pacientes e cirurgiões-dentistas. O tabagismo é reconhecido como um importante fator de risco para o desenvolvimento dessas doenças interferindo diretamente no mecanismo inflamatório básico assim dificultando o diagnóstico. Esses dispositivos eletrônicos, foram introduzidos no mercado como alternativa para o cigarro tradicional, mas tornaram-se populares entre os jovens por serem aromatizados, atraentes e a falsa percepção de efeitos negativos à saúde. A atividade de cigarros eletrônicos vem sendo promovida como uma alternativa mais saudável, contudo não deve ser confundida com inofensivo. A literatura aponta que os agentes aromatizantes dos cigarros eletrônicos podem ter efeitos adversos semelhantes aos produtos do tabaco e que, assim como o tabagismo, aumentam as chances de riscos à periodontite, além de causar alterações pulmonares e cardiovasculares. Os pacientes fumantes de cigarros tradicionais e eletrônicos respondem menos favoravelmente ao tratamento periodontal não cirúrgico quando comparados aos não fumantes.

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“…This suggests that although nicotine is not a carcinogen and promotes the development of oral cancer through EGFR, P44/42 mitogen-activated protein kinase (ERK) and protein kinase B (AKT) [7]. Nicotine also significantly increased autophagy expression in human periodontal cells (hPDLC) via α7nAChR, which in turn regulated the liberation of inflammatory factors Interleukin-1β (IL-1β), and IL-8 from hPDLCs [8]. An in vitro study showed that a certain concentration of nicotine significantly inhibited the proliferation of oral epithelial cells and that nicotine concentration-dependently decreased the expression of Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) and TNF-α-induced cell surface expression of intercellular adhesion molecule-1 (ICAM-1).…”

Section: Composition Analysis Of Electronic Cigarettes and Traditiona...mentioning

confidence: 99%

Electronic cigarettes, traditional cigarettes, and oral health

Liu

Yue²,

Zhao³

et al. 2022

International Conference on Biomedical and Intelligent Systems (IC-BIS 2022)

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Background: Electronic cigarettes are increasingly preferred by young people today, and most people use them to quit smoking. However, the impact of e-cigarettes on human oral health is still controversial. It is debatable whether e-cigarettes are as safe, regulated, and harmless as one might believe, and whether they have potential side effects on the human body. Objective: In this paper, we reviewed the literature related to traditional cigarettes and e-cigarettes and oral health in recent years, and investigate the impact of e-cigarettes and traditional cigarettes on oral health from four aspects: analysis of the composition of e-cigarettes and traditional cigarettes, effects on oral cells, oral flora, and clinical oral health. Conclusion: Both e-cigarettes and traditional cigarettes have an impact on a person's oral health. However, extensive experimental data is needed to prove whether e-cigarette aerosol is more harmful to the oral cavity than cigarette smoke (CS).

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Nicotine regulates autophagy of human periodontal ligament cells through α7 nAchR that promotes secretion of inflammatory factors IL-1β and IL-8

Cited by 9 publications

References 54 publications

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Considerações atuais sobre o uso dos cigarros eletrônicos relacionados às doenças periodontais: revisão da literatura

Electronic cigarettes, traditional cigarettes, and oral health

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