Nicotine Production by Tissue Cultures of Tobacco as Influenced by Various Culture Parameters

Plant Cell Biotechnology

“…Relevant production conditions include nutrient availability, other biotic and abiotic elements introduced for co-culturing, pH, degree of mixing and aeration, and factors introduced with the inoculum (internal culture conditions) as well as shearing force, agitating frequency, temperature and light (external conditions) (Rokem and Goldberg 1985;Roper et al 1985).…”

The application of root cultures to problems of biological chemistry

“…Interestingly, the degradation of 13 did not appear to be stimulated. This is surprising as, although some carefully selected suspension cultures do make 13, 45 they can also show a strong degradative capacity. 41 Loss of biosynthetic activity in both types of culture 44,46 has been shown to be due to the rapid disappearance of the enzyme for the N-methylation of 20, putrescine N-methyl transferase (PMT).…”

Plant Cells as Producers of Secondary Compounds

“…Relevant production conditions include nutrient availability, other biotic and abiotic elements introduced for co-culturing, pH, degree of mixing and aeration, and factors introduced with the inoculum (internal culture conditions) as well as shearing force, agitating frequency, temperature and light (external conditions) (Rokem and Goldberg 1985;Roper et al 1985). Relevant production conditions include nutrient availability, other biotic and abiotic elements introduced for co-culturing, pH, degree of mixing and aeration, and factors introduced with the inoculum (internal culture conditions) as well as shearing force, agitating frequency, temperature and light (external conditions) (Rokem and Goldberg 1985;Roper et al 1985).…”