Nicotine elevates sperm motility and induces<i>Pfn1</i>promoter hypomethylation in mouse testis

Dai, Jingbo; Zhan, Chang-Sheng; Xu, Wangjie; Wang, Z; Nie, Dongsheng; Zhao, Xianglong; Zhang, D; Gu, Yuwei; Wang, L; Chen, Zhiao; Qiao, Zhongdong

doi:10.1111/andr.12072

Cited by 21 publications

(9 citation statements)

References 34 publications

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“…There is evidence that smoking induces significant changes of DNA methylation in sperm 52, 53 . DNA methylation is associated with changes in the gene expression profile.…”

Section: Discussionmentioning

confidence: 99%

Maternal smoking and autism spectrum disorder: meta-analysis with population smoking metrics as moderators

Jung

Lee

McKee

et al. 2017

Sci Rep

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While exposure to nicotine during developmental periods can significantly affect brain development, studies examining the association between maternal smoking and autism spectrum disorder (ASD) in offspring have produced conflicting findings, and prior meta-analyses have found no significant association. Our meta-analysis used a novel approach of investigating population-level smoking metrics as moderators. The main meta-analysis, with 22 observational studies comprising 795,632 cases and 1,829,256 control participants, used a random-effects model to find no significant association between maternal smoking during pregnancy and ASD in offspring (pooled odds ratio (OR) = 1.16, 95% CI: 0.97–1.40). However, meta-regression analyses with moderators were significant when we matched pooled ORs with adult male smoking prevalence (z = 2.55, p = 0.01) in each country, using World Health Organization data. Our study shows that using population-level smoking metrics uncovers significant relationships between maternal smoking and ASD risk. Correlational analyses show that male smoking prevalence approximates secondhand smoke exposure. While we cannot exclude the possibility that our findings reflect the role of paternal or postnatal nicotine exposure, as opposed to maternal or in utero nicotine exposure, this study underlines the importance of investigating paternal and secondhand smoking in addition to maternal smoking in ASD.

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“…There is evidence that smoking induces significant changes of DNA methylation in sperm 52, 53 . DNA methylation is associated with changes in the gene expression profile.…”

Section: Discussionmentioning

confidence: 99%

Maternal smoking and autism spectrum disorder: meta-analysis with population smoking metrics as moderators

Jung

Lee

McKee

et al. 2017

Sci Rep

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“…Nicotine in tobacco smoke is one of the most toxic substances that cause addiction and can readily be detected in the serum and semen of smokers (Dai et al, ). Shown to act as a powerful oxidising agent, nicotine may have an impact on sperm function by altering sperm plasma membrane and DNA integrity in humans (Arabi, ).…”

Section: Components Of Cigarette Smokementioning

confidence: 99%

“…In mouse testis, nicotine exposure induced hypomethylation of profilin 1 ( Pfn1) gene encoding a protein acting in the regulation of cytoskeleton. Pfn1 promoter hypomethylation resulted in its overexpression, promoting actin polymerisation and presumably increasing sperm motility in mice (Dai et al, ).…”

Section: Cigarette Smoking and Epigenetic Alterationsmentioning

confidence: 99%

“…Smoking‐induced genetic alterations have an influence on fertility through chromosomal alterations (Härkönen, Viitanen, Larsen, Bonde, & Lähdetie, ; Pereira et al, ; Rubes et al, ), mutations (Yauk et al, ), polymorphisms (Ji, Yan, Liu, Qu, & Gu, ; Yarosh, Kokhtenko, Starodubova, Churnosov, & Polonikov, ; Yu et al, ), sister chromatid exchanges (SCE) (Papachristou et al, ), micronuclei (Lähdetie, ; Milosevic‐Djordjevic, Stosic, Grujicic, Zelen, & Sazdanovic, ) and DNA damage (Elshal, El‐Sayed, Elsaied, El‐Masry, & Kumosani, ; Fraga et al, ; Viloria et al, ; Zenzes, Bielecki, & Reed, ) (Figure ). Over the last decades, the association of epigenetic factors (such as DNA methylation, chromatin remodelling and noncoding RNAs) with male infertility‐related smoking has also been investigated (Dai et al, ; Dong et al, ; La Maestra, De Flora, & Micale, ; Yu, Ding, et al, ; Yu, Qi, et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Smoking-induced genetic and epigenetic alterations in infertile men

et al. 2018

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Male fertility rates have shown a progressive decrease in both developing and industrialised countries in the past 50 years. Clinical and epidemiological studies have demonstrated controversial results about the harmful effects of cigarette smoking on seminal parameters. Some studies could not establish a negative effect by tobacco smoking on sperm quality and function, whereas others have found a significant reduction in sperm quality and function. This study reviews the components in cigarette smoke and discusses the effects of smoking on male fertility by focusing extensively on smoking-induced genetic and epigenetic alterations in infertile men. Chromosomal aneuploidies, sperm DNA fragmentation and gene mutations are discussed in the first section, while changes in DNA methylation, chromatin remodelling and noncoding RNAs are discussed in the second section as part of epigenetic alterations.

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“…The hazardous effects of tobacco smoking on male fertility have long been studied but the underlying molecular mechanism remains vague (Dai et al 2015b). Nicotine alters mouse epididymal protein profile…”

Section: Discussionmentioning

confidence: 99%

Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa

Dai¹,

Xu²,

Zhao³

et al. 2016

Reproduction

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Many studies have revealed the hazardous effects of cigarette smoking and nicotine exposure on male fertility, but the actual, underlying molecular mechanism remains relatively unclear. To evaluate the detrimental effects of nicotine exposure on the sperm maturation process, two-dimensional gel electrophoresis and mass spectrometry analyses were performed to screen and identify differentially expressed proteins from the epididymal tissue of mice exposed to nicotine. Data mining analysis indicated that 15 identified proteins were mainly involved in the molecular transportation process and the polyol pathway, indicating impaired epididymal secretory functions. Experiments in vitro confirmed that nicotine inhibited tyrosine phosphorylation levels in capacitated spermatozoa via the downregulated seminal fructose concentration. Sord, a key gene encoding sorbitol dehydrogenase, was further investigated to reveal that nicotine induced hyper-methylation of the promoter region of this gene. Nicotine-induced reduced expression of Sord could be involved in impaired secretory functions of the epididymis and thus prevent the sperm from undergoing proper maturation and capacitation, although further experiments are needed to confirm this hypothesis.

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Nicotine elevates sperm motility and inducesPfn1promoter hypomethylation in mouse testis

Cited by 21 publications

References 34 publications

Maternal smoking and autism spectrum disorder: meta-analysis with population smoking metrics as moderators

Maternal smoking and autism spectrum disorder: meta-analysis with population smoking metrics as moderators

Smoking-induced genetic and epigenetic alterations in infertile men

Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa

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