Nicotine-derived NNK induces the stemness enrichment of CRC cells through regulating the balance of DUSP4-ERK1/2 feedback loop

Chen, Yansu; Wang, Qinzhi; Cao, Lin; Tang, Yu; Yao, Meixue; Bi, Haoran; Huang, Yefei; Sun, Guochen; Song, Jun

doi:10.1016/j.ecoenv.2021.112057

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…The results revealed a high expression of DUSP4, BATF, and TNFRSF18 in cytotoxic T cells derived from CRC tissues, all of which have been reported to be associated with malignant progression of tumors (Fig. 4H) [45][46][47]. The results of pathway enrichment analysis further supported the signi cant activation of energy metabolism-related pathways and the signi cant inactivation of immune-related signaling pathways in cytotoxic T cells derived from CRC tissues (Fig.…”

Section: Epithelial Cells Inhibited Activation Of T Cells Via Mif/cd7...supporting

confidence: 53%

Identification of a novel T cell-related signature to predict prognosis in colorectal cancer via integrating single-cell and bulk RNA sequencing

Zhang,

Zhu,

Wen

et al. 2024

Preprint

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Background: T cells, the key mediators of tumor destruction, have a considerable impact on tumor prognosis. However, the clinical significance of T cell-associated biomarkers in colorectal cancer (CRC) haven’t been well understood. The aim of this study was to investigate the expression profile of T cell marker genes in CRC and develop a prognostic signature based on these genes. Methods: Single-cell RNA-sequencing (scRNA-seq) data were retrieved from the Gene Expression Omnibus (GEO) database. Bulk RNA-sequencing data and clinical information were downloaded from The Cancer Genome Atlas (TCGA) and GEO databases. We firstly conducted a comprehensive analysis of scRNA-seq data to investigate the heterogeneity of various cells in the CRC tumor microenvironment (TME). Then, we performed cell-cell communication analysis and cell trajectory analysis to explore the intercellular interactions and functional changes of T cells. By combing the bulk RNA-seq data, a T-cell related gene signature was eventually constructed and its predictive ability was determined by the Kaplan–Meier (K-M), and receiver operating characteristic (ROC) curves in three independent cohorts. Results: ScRNA-seq data obtained from the GEO database were re-integrated and analyzed, resulting in 23 cell clusters. Distinct cell clusters were annotated using extensively reported cell markers. The CellChat algorithm revealed that tumor cells suppress the cellular function of tumor-infiltrating T cells through the MIF/CD74 pathway. The evolutionary trajectory of tumor-infiltrating T cells was elucidated by the CytoTRACE and monocle2 algorithms. Eventually, a prognostic prediction model based on 5 T cell-related genes was constructed using single-cell and bulk RNA sequencing data. The validation results from several independent CRC cohorts indicated that the 5 T cell-related genes prognostic model could accurately predict the survival outcomes of CRC patients, providing new evidence for precision treatment in CRC. Conclusions: Our study not only offers prospects for a better understanding of the cellular heterogeneity of TME, but also provides a useful tool for stratifying patients with different prognoses and facilitating personalized treatment.

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Section: Epithelial Cells Inhibited Activation Of T Cells Via Mif/cd7...supporting

confidence: 53%

Identification of a novel T cell-related signature to predict prognosis in colorectal cancer via integrating single-cell and bulk RNA sequencing

Zhang,

Zhu,

Wen

et al. 2024

Preprint

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“…DUSP4 is considered a biomarker in various cancer studies, but its precise role is controversial. Previous studies have been performed mainly on breast cancer [19][20][21][22], colorectal cancer [23][24][25][26], lung cancer [27,28], and thyroid papillary carcinoma [12,13,29,30]. Many studies reported that DUSP4 showed an oncogenic effect.…”

Section: Discussionmentioning

confidence: 99%

Loss of DUSP4 Expression as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma

et al. 2021

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Dual-specificity protein phosphatase 4 (DUSP4) is a negative regulator of mitogen-activated protein kinases. The prognostic impact of DUSP4 expression in renal cell carcinoma is not well studied. Therefore, we evaluated the clinicopathological implications of DUSP4 expression in clear cell renal cell carcinoma by performing immunohistochemistry (IHC). The clinical outcome according to DUSP4 expression was evaluated through survival analyses, and the association between mRNA expression and prognosis was confirmed by online analysis (Kaplan–Meier plotter). Loss of DUSP4 expression was noted in most histological subtypes of renal cell carcinoma. Loss of DUSP4 expression in clear cell renal cell carcinoma was significantly correlated with old age (p = 0.033), high histologic grade (p < 0.001), tumor necrosis (p < 0.001), and high pT category (p < 0.001). In survival analysis, loss of DUSP4 expression was associated with poor clinical outcomes in cancer-specific survival and recurrence-free survival (p = 0.010 and p = 0.007, respectively). Upon TCGA data analysis, patients with low DUSP4 mRNA expression showed a shorter overall survival (p = 0.023). These results suggest that loss of DUSP4 expression can be used as a potential biomarker for predicting clinical outcomes in clear cell renal cell carcinoma patients.

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GCF2 mediates nicotine-induced cancer stemness and progression in hepatocellular carcinoma

Li,

Tuo,

Cheng

et al. 2024

Ecotoxicology and Environmental Safety

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Nicotine-derived NNK induces the stemness enrichment of CRC cells through regulating the balance of DUSP4-ERK1/2 feedback loop

Cited by 5 publications

References 37 publications

Identification of a novel T cell-related signature to predict prognosis in colorectal cancer via integrating single-cell and bulk RNA sequencing

Identification of a novel T cell-related signature to predict prognosis in colorectal cancer via integrating single-cell and bulk RNA sequencing

Loss of DUSP4 Expression as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma

GCF2 mediates nicotine-induced cancer stemness and progression in hepatocellular carcinoma

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