Nicotine can modulate the effects of the mesenchymal stem cells on neutrophils

Pourtayeb, Samira; Froushani, Seyyed Meysam Abtahi

doi:10.1016/j.advms.2016.07.006

Cited by 11 publications

(11 citation statements)

References 27 publications

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“…It was revealed that MSCs in tissues significantly increased the lifespan of neutrophils by protecting them from apoptosis (10,23). The MTT reduction test is a simple and rapid way of evaluating cell vitality (23). In the present survey, the data showed that the vitality of neutrophils, primed with CM, was higher than their survival rate in the absence of CM.…”

Section: Discussionmentioning

confidence: 54%

“…Also, MSCs primed with caffeine at low to moderate concentrations (0.1 and 0.5 µM) could preserve the potential of neutral red ingestion by neutrophils and appointed the MSCs' potential to protect neutrophils from apoptotic death. Nonetheless, caffeine at a high concentration (100 µM) intervenes with some schema of the mutual communication between MSCs and neutrophils (23).…”

Section: Discussionmentioning

confidence: 99%

“…Neutrophils have a short lifespan and therefore, their homeostasis and turnover should be highly regulated (38). It was revealed that MSCs in tissues significantly increased the lifespan of neutrophils by protecting them from apoptosis (10,23). The MTT reduction test is a simple and rapid way of evaluating cell vitality (23).…”

Section: Discussionmentioning

confidence: 99%

“…Multiple documents, similar to our results, exhibited that MSCs or their CM could change significantly the inflammatory function of neutrophils, like respiratory burst and MPO activity. (23,24,44). Interestingly, it has been proposed that similar to macrophages, migrating neutrophils are plastic and diverse and can possess different phenotypes depending on environmental factors (44).…”

Section: Discussionmentioning

confidence: 99%

“…The crosstalk between MSCs and neutrophils has been reported in some valuable studies (21)(22)(23)(24). Nonetheless, there are no or very little data about the role of theobromine as a routine food material in the crosstalk between MSCs and neutrophils.…”

Section: Introductionmentioning

confidence: 99%

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Conditioned Medium of Mesenchymal Stem Cells Pulsed with Theobromine Can Instruct Anti-Inflammatory Neutrophils

Froushani

Abbasi

2020

Zahedan J Res Med Sci

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Background: Both adenosine signaling and phosphodiesterase inhibitor agents can alter the survivability and differentiation potential of Mesenchymal Stem Cells (MSCs). On the other hand, the crosstalk between MSCs and immunocytes like neutrophils is clear. Objectives: Here, we examined the consequence of inflammatory functions of neutrophils after co-culture with conditioned MSC Medium (CM) whose MSCs had previously been pulsed with theobromine. Methods: Mesenchymal stem cells were separated and characterized by the bone marrow of Wistar rats. These cells were primed with different concentrations of theobromine (0, 10, 50, and 100 μM) for 48 hours. Neutrophils were primed with CM for four hours and their performance was examined. Results: CM primed with theobromine at low to moderate concentrations protected the neutral red removal by neutrophils and potentiated CM potential to support neutrophils from apoptosis. CM from MSC primed with theobromine augmented the phagocytosis potential of co-cultured neutrophils. Conversely, CM isolated from MSCs pulsed with theobromine reduced the production of potentially noxious reactive oxygen species and myeloperoxidase activity more profoundly than did CM from un-pulsed MSCs. Conclusions: Conditioned medium of MSCs pulsed with theobromine can instruct anti-inflammatory neutrophils.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%