Nicotine biosynthesis is regulated by two more layers: Small and long non-protein-coding RNAs

Xie, Jiahua; Fan, Longjiang

doi:10.1080/15592324.2016.1184811

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…These interesting results indicate that the trend of nicotine levels is stably controlled by tobacco plants. The mechanism behind this trend stability of nicotine remain uninvestigated, although its biosynthetic pathway has been studied intensively(Xie and Fan, 2016; Kajikawa et al, 2017). Furthermore, it has been vastly documented that nicotine is exclusively biosynthesized in roots and then transported to leaves for storage.…”

Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics of Nicotiana tabacum Grown in United States and India Characterizes the Association of Plant Metabolomes With Natural Climate and Geography

Gandra

Manoharlal

et al. 2019

Front. Plant Sci.

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Climate change and geography affect all the living organisms. To date, the effects of climate and geographical factors on plant metabolome largely remain open for worldwide and local investigations. In this study, we designed field experiments with tobacco (Nicotiana tabacum) in India versus USA and used untargeted metabolomics to understand the association of two weather factors and two different continental locations with respect to tobacco metabolism. Field research stations in Oxford, North Carolina, USA, and Rajahmundry, Andhra Pradesh India were selected to grow a commercial tobacco genotype (K326) for 2 years. Plant growth, field management, and leaf curing followed protocols standardized for tobacco cultivation. Gas chromatography–mass spectrometry based unbiased profiling annotated 171 non-polar and 225 polar metabolites from cured tobacco leaves. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) showed that two growing years and two field locations played primary and secondary roles affecting metabolite profiles, respectively. PCA and Pearson analysis, which used nicotine, 11 other groups of metabolites, two locations, temperatures, and precipitation, revealed that in North Carolina, temperature changes were positively associated with the profiles of sesquiterpenes, diterpenes, and triterpenes, but negatively associated with the profiles of nicotine, organic acids of tricarboxylic acid, and sugars; in addition, precipitation was positively associated with the profiles of triterpenes. In India, temperature was positively associated with the profiles of benzenes and polycyclic aromatic hydrocarbons, but negatively associated with the profiles of amino acids and sugar. Further comparative analysis revealed that nicotine levels were affected by weather conditions, nevertheless, its trend in leaves was independent of two geographical locations and weather changes. All these findings suggested that climate and geographical variation significantly differentiated the tobacco metabolism.

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Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics of Nicotiana tabacum Grown in United States and India Characterizes the Association of Plant Metabolomes With Natural Climate and Geography

Gandra

Manoharlal

et al. 2019

Front. Plant Sci.

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“…Nicotine, one of the major components of cigarette smoking, has a role on cell proliferation of colorectal cancer, as well as of cell lines derived from several different cancer types [19]. It can suppress cell apoptosis induced by extracellular stress stimuli both in normal cells, such as vascular endothelial and smooth muscle cells [5], and in several human cancer cell lines derived from various organs [4,20]. Although there are a few studies on the mechanism of nicotine carcinogenesis, the effects of nicotine on lung cancer is still needed to fulfill in detail.…”

Section: Discussionmentioning

confidence: 99%

Nicotine promotes the development of non-small cell lung cancer through activating LINC00460 and PI3K/Akt signaling

2019

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Objective: Nicotine, the main ingredient in tobacco, is identified to facilitate tumorigenesis and accelerate metastasis in tumor. Studies in recent years have reported that long intergenic non-protein coding RNA 460 (LINC00460) is strongly associated with lung cancer poor prognosis and nicotine dependence. Nonetheless, it is unclear whether nicotine promotes the development of lung cancer through activation of LINC00460. Methods: We determined that LINC00460 expression in lung cancer tissues and the prognosis in patients with non-small cell lung carcinoma (NSCLC) using Gene Expression Profiling Interactive Analysis (GEPIA) website and The Cancer Genome Atlas (TCGA) database. Through in vitro experiments, we studied the effects of nicotine on LINC00460 in NSCLC cells lines using Cell Counting Kit-8 (CCK-8), transwell test, flow cytometry, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blot assays. Results: We identified the significant up-regulated expression level of LINC00460 in NSCLC tissues and cell lines, especially, the negative correlation of LINC00460 expression level with overall survival (OS). In in vitro experiments, LINC00460 was overexpressed in NSCLC cell lines under nicotine stimulation. Nicotine could relieve the effect of LINC00460 knockdown on NSCLC cell proliferation, migration and apoptosis. The same influence was observed on PI3K/Akt signaling pathway. Conclusions: In summary, this is the first time to examine the potential roles of LINC00460 in lung cancer cell proliferation, migration and apoptosis induced by nicotine. This may help to develop novel therapeutic strategies for the prevention and treatment of metastatic tumors from cigarette smoke-caused lung cancer by blocking the nicotine-activated LINC00460 pathway.

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“…Interaction studies so far in plants mostly focused on two types of RNAs, such as miRNAs and their mRNA targets [1], circRNAs and their parental genes [32], and miRNAs and their eTM lncRNAs [2]. Regarding regulation of nicotine biosynthesis and other secondary metabolism in tobacco, a role of ncRNAs has rarely been demonstrated [33]. The present work investigated the dynamic changes of mRNAs, miRNAs, circRNAs and lncRNAs, and their interactions in response to topping treatment in tobacco, with a focus on coding and non-coding RNAs related to nicotine biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

A transcriptomic profile of topping responsive non-coding RNAs in tobacco roots (Nicotiana tabacum)

et al. 2019

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BackgroundNon-coding RNAs (ncRNAs), including microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs), accomplish remarkable variety of biological functions. However, the composition of ncRNAs and their interactions with coding RNAs in modulating and controlling of cellular process in plants is largely unknown. Using a diverse group of high-throughput sequencing strategies, the mRNA, miRNA, lncRNA and circRNA compositions of tobacco (Nicotiana tabacum) roots determined and their alteration and potential biological functions in response to topping treatment analyzed.ResultsA total of 688 miRNAs, 7423 non-redundant lncRNAs and 12,414 circRNAs were identified, among which, some selected differentially expressed RNAs were verified by quantitative real-time PCR. Using the differentially expressed RNAs, a co-expression network was established that included all four types of RNAs. The number of circRNAs identified were higher than that of miRNAs and lncRNAs, but only two circRNAs were present in the co-expression network. LncRNAs appear to be the most active ncRNAs based on their numbers presented in the co-expression network, but none of them seems to be an eTM (endogenous Target Mimicry) of miRNAs. Integrated with analyses of sequence interaction, several mRNA-circRNA-miRNA interaction networks with a potential role in the regulation of nicotine biosynthesis were uncovered, including a QS-circQS-miR6024 interaction network. In this network miR6024 was significantly down-regulated, while the expression levels of its two targets, circQS and its host gene QS, were sharply increased following the topping treatment.ConclusionsThese results illustrated the transcriptomic profiles of tobacco roots, the organ responsible for nicotine biosynthesis. mRNAs always play the most important roles, while ncRNAs are also expressed extensively for topping treatment response, especially circRNAs are the most activated in the ncRNA pool. These studies also provided insights on the coordinated regulation module of coding and non-coding RNAs in a single plant biological sample. The findings reported here indicate that ncRNAs appear to form interaction complex for the regulation of stress response forming regulation networks with transcripts involved in nicotine biosynthesis in tobacco.

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Nicotine biosynthesis is regulated by two more layers: Small and long non-protein-coding RNAs

Cited by 9 publications

References 31 publications

Untargeted Metabolomics of Nicotiana tabacum Grown in United States and India Characterizes the Association of Plant Metabolomes With Natural Climate and Geography

Untargeted Metabolomics of Nicotiana tabacum Grown in United States and India Characterizes the Association of Plant Metabolomes With Natural Climate and Geography

Nicotine promotes the development of non-small cell lung cancer through activating LINC00460 and PI3K/Akt signaling

A transcriptomic profile of topping responsive non-coding RNAs in tobacco roots (Nicotiana tabacum)

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