Nicotine Attenuates Macrophage Infiltration in Rat Lung Allografts

Hirschburger, Markus; Zakrzewicz, Anna; Kummer, Wolfgang; Padberg, Winfried

doi:10.1016/j.healun.2009.02.005

Cited by 11 publications

(13 citation statements)

References 53 publications

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“…Immunohistochemical staining for markers of inflammatory cells was performed as described recently [34]. In detail, monoclonal antibodies (mAbs) ED1, ED2 and R73 were purchased from Serotec (Dü sseldorf, Germany).…”

Section: Immunohistochemistrymentioning

confidence: 99%

All-trans retinoic acid results in irregular repair of septa and fails to inhibit proinflammatory macrophages

Seifart

Muyal

Plagens

et al. 2011

European Respiratory Journal

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All-trans retinoic acid (ATRA) is controversially discussed in emphysema therapy. We re-evaluated ATRA in the elastase model and hypothesised that beneficial effects should be reflected by increased alveolar surface area, elastin expression and downregulation of inflammatory mediators and matrix metalloproteinases (MMPs).Emphysema was induced by porcine pancreatic elastase versus saline in Sprague-Dawley rats. On days 26-37, rats received daily intraperitoneal injections with ATRA (500 mg?kg -1 body weight)versus olive oil. Lungs were removed at day 38. Rat alveolar epithelial L2 cells were incubated with/without elastase followed by ATRA-or vehicle-treatment, respectively. ATRA only partially ameliorated structural defects. Alveolar walls exhibited irregular architecture: increased arithmetic mean thickness, reduction in surface coverage by alveolar epithelial cells type II. ATRA only partially restored reduced soluble elastin. It tended to increase the ratio of ED1 + :ED2 + macrophages. Bronchoalveolar lavage (BAL) cells exhibited a proinflammatory state and high expression of interleukin-1b, cytokine-induced neutrophil chemoattractant-1, tumour necrosis factor-a, nuclear factor-kB, MMP-2, MMP-9, MMP-12, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in emphysema, with ATRA exerting only few effects. MMP-7 was highly induced by ATRA in healthy but not in emphysematous lungs. ATRA reduced both MMP-2 and TIMP-1 activity in BAL fluid of emphysematous lungs. ATRA-therapy may bear the risk of unwanted side-effects on alveolar septal architecture in emphysematous lungs.

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Section: Immunohistochemistrymentioning

confidence: 99%

All-trans retinoic acid results in irregular repair of septa and fails to inhibit proinflammatory macrophages

Seifart

Muyal

Plagens

et al. 2011

European Respiratory Journal

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“…The macrophages were stained by immunohistochemistry for ED1, as described previously (13,46). Positively stained cells were counted in a blinded fashion in 20 high-power fields (HPFs) per each section and expressed as the number of cells per HPF.…”

Section: Staining For Infiltrating Ed1-positive Macrophagesmentioning

confidence: 99%

Inhaled Hydrogen Gas Therapy for Prevention of Lung Transplant-Induced Ischemia/Reperfusion Injury in Rats

Kawamura¹,

Huang²,

Lee³

et al. 2010

Transplantation Journal

102

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Background. Successful abrogation of ischemia/reperfusion (I/R) injury of lung grafts could significantly improve short-and long-term outcomes for lung transplant (LTx) recipients. Hydrogen gas has potent antioxidant and antiapoptotic properties and has been recently used in number of experimental and clinical studies. The purpose of this research was to investigate whether inhaled hydrogen gas could reduce graft I/R injury during lung transplantation. Methods. Orthotopic left LTxs were performed in syngenic Lewis rats. Grafts were perfused with and stored in low potassium dextran solution at 4°C for 6 hr. The recipients received 100% O 2 or 98% O 2 with 2% N 2 , 2% He, or 2% H 2 during surgery and 1 hr after reperfusion. The effects of hydrogen were assessed by functional, pathologic, and molecular analysis. Results. Gas exchange was markedly impaired in animals exposed to 100% O 2 , 2% N 2 , or 2% He. Hydrogen inhalation attenuated graft injury as indicated by significantly improved gas exchange 2 hr after reperfusion. Graft lipid peroxidation was significantly reduced in the presence of hydrogen, demonstrating antioxidant effects of hydrogen in the transplanted lungs. Lung cold I/R injury causes the rapid production and release of several proinflammatory mediators and epithelial apoptosis. Exposure to 2% H 2 significantly blocked the production of several proinflammatory mediators and reduced apoptosis with induction of the antiapoptotic molecules B-cell lymphoma-2 and B-cell lymphoma-extra large. Conclusion. Treatment of LTx recipients with inhaled hydrogen can prevent lung I/R injury and significantly improve the function of lung grafts after extended cold preservation, transplant, and reperfusion.

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“…It is unlikely that this response is due to direct changes in resident populations as both sympathetic blockade [80][81][82][83][84][85] and radiation [63,86,87] have been shown to inhibit mitogen-induced proliferation and promote apoptosis in splenocytes. Instead, it seems much more likely that this response is due to changes in immunocyte trafficking.…”

Section: Impact Of Sympathetic Blockade On the Radiation Responsementioning

confidence: 99%

“…Not only is trafficking through the splenic vasculature controlled by sympathetic innervation [90], but immunocytes, including lymphocytes, monocytes, and granulocytes, are known to express nicotinic receptors [91][92][93]. Furthermore, other investigators have demonstrated that administration of nicotine can inhibit leukocyte infiltration at inflamed sites [82][83][84][85].…”

Section: Impact Of Sympathetic Blockade On the Radiation Responsementioning

confidence: 99%

Chlorisondamine, a sympathetic ganglionic blocker, moderates the effects of whole-body irradiation (WBI) on early host defense to a live bacterial challenge

et al. 2015

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Nicotine Attenuates Macrophage Infiltration in Rat Lung Allografts

Cited by 11 publications

References 53 publications

All-trans retinoic acid results in irregular repair of septa and fails to inhibit proinflammatory macrophages

All-trans retinoic acid results in irregular repair of septa and fails to inhibit proinflammatory macrophages

Inhaled Hydrogen Gas Therapy for Prevention of Lung Transplant-Induced Ischemia/Reperfusion Injury in Rats

Chlorisondamine, a sympathetic ganglionic blocker, moderates the effects of whole-body irradiation (WBI) on early host defense to a live bacterial challenge

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