Nicotinamide riboside reduces cardiometabolic risk factors and modulates cardiac oxidative stress in obese Wistar rats under caloric restriction

Castro, Juliane Machado de; Assumpção, José Antônio Fagundes; Stein, Dirson João; Toledo, Roberta Ströher; Silva, Lisiane Santos da; Caumo, Wolnei; Carraro, Cristina Campos; Araújo, Alex Sander da Rosa; Torres, Iraci Lucena da Silva

doi:10.1016/j.lfs.2020.118596

Cited by 16 publications

(16 citation statements)

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“…As far as we know, these preliminary data are the first in the literature to evidence the combined effects of NR and CR on muscle tissue, especially in obesity conditions. We have previously shown that 28 days of CR in rats with obesity effectively reverses obesogenic indicators ( 12 ). In fact, CR is undeniably effective in the short term and medium term to reduce BW and adiposity ( 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…The data presented in this study were obtained and continued from our previous experiments ( 12 ). A full version of the methods is also published in this study.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, these three subgroups were further subdivided into the vehicle and NR-supplemented animals, resulting in six subgroups ( Figure 1 ). Noteworthy, CAFD for 6 weeks was efficient in inducing obesity ( 12 ). CAFD is a robust and translational obesity model that aims to offer animals the same and main obesity-generating hypercaloric foods in humans, for example, flavored snacks, stuffed crackers, processed meats, and sugar-sweetened beverages ( 14 ).…”

Section: Methodsmentioning

confidence: 99%

“…Thus, the CR protocol consisted of replacing a hypercaloric diet (CAFD) with a standard diet (SD) for 4 weeks, starting immediately after the establishment of obesity. This process promoted, on average, a 62% reduction in calories ingested when compared with calories ingested during CAFD access ( 12 ). Nutritional characteristics of both diets are presented in Table 1 , and complete nutritional information in Supplementary Material .…”

Section: Methodsmentioning

confidence: 99%

“…In line with this, nicotinamide riboside (NR), a dietary compound analogous to vitamin B3, has been shown in recent experiments as effective for weight loss and SMT health maintenance ( 9 – 11 ). We have previously shown that supplementation with NR positively modulates obesity-related parameters in both obese rats and obese rats under CR, including reduced BW gain and reduced adiposity ( 12 ), unaware of whether this process affected SMT or neuroinflammatory parameters. As studies on this topic are really scarce, with no clinical or preclinical evidence reporting its potential effects on muscle mass or neuroinflammatory events when associated with a CR strategy under obesity conditions, in this study, we aimed to investigate whether NR supplementation, combined or not with CR, affects muscle mass and hypothalamic inflammatory parameters of rats with obesity induced by a hypercaloric diet model.…”

Section: Introductionmentioning

confidence: 99%

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Nicotinamide Riboside Neutralizes Hypothalamic Inflammation and Increases Weight Loss Without Altering Muscle Mass in Obese Rats Under Calorie Restriction: A Preliminary Investigation

et al. 2021

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Obesity treatments, such as calorie restriction (CR), eventually lead to muscle wasting and higher rates of neuroinflammation, whereas hypothalamic inflammatory conditions impair body weight (BW) control. Nicotinamide riboside (NR) has been proposed against obesity but with little evidence on skeletal muscle tissue (SMT) and neuroinflammation. Therefore, we aimed to investigate the effects of CR on SMT and on hypothalamic inflammatory biomarkers in obese adult male Wistar rats, and whether NR supplementation alone or in combination with CR affects these parameters. Obesity was induced in rats through a cafeteria diet for 6 weeks. After that, a group of obese rats was exposed to CR, associated or not associated with NR supplementation (400 mg/kg), for another 4 weeks. As a result, obese rats, with or without CR, presented lower relative weight of SMT when compared with eutrophic rats. Rats under CR presented lower absolute SMT weight compared with obese and eutrophic rats, in addition to presenting elevated hypothalamic levels of TNF-α. NR supplementation, in all groups, enhanced weight loss and increased relative weight of the SMT. Furthermore, in animals under CR, NR reversed increases TNF-α levels in the hypothalamus. In this study, these data, although succinct, are the first to evidence the effects of NR on SMT and neuroinflammation when associated with CR, especially in obesity conditions. Therefore, this provides preliminary support for future studies in this investigative field. Furthermore, NR emerges as a potential adjuvant for preventing muscle mass loss in the weight loss processes.

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Section: Discussionmentioning

confidence: 99%

“…The data presented in this study were obtained and continued from our previous experiments ( 12 ). A full version of the methods is also published in this study.…”

Section: Methodsmentioning

confidence: 99%