Nicotinamide Mononucleotide Restores the Meiotic Competency of Porcine Oocytes Exposed to Ethylene Glycol Butyl Ether

Miao, Yun; Li, Xinyu; Shi, Xiaoyan; Gao, Qian; Chen, Jingyue; Wang, Rui; Fan, Yong; Xiong, Bo

doi:10.3389/fcell.2021.628580

Cited by 11 publications

(9 citation statements)

References 38 publications

(42 reference statements)

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“…These data indicated that NMN inhibited the ROS accumulation in oocytes of HFD mice which further improved oocyte quality of HFD mice. It was reported that the actin dynamics is involved in oocyte quality control and NMN could recover the abnormal actin dynamics in porcine oocytes 34 . In line with this, we found that high fat diet disturbed the integrity of actin in oocytes and NMN could restore the actin dynamics in the HFD mice oocytes.…”

Section: Discussionsupporting

confidence: 88%

Administration of nicotinamide mononucleotide improves oocyte quality of obese mice

et al. 2022

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Objectives: Obesity has become a common health concern around the world. Maternal obesity could cause poor reproductive outcomes due to chronic ovarian inflammation and decreased oocyte quality. However, the strategies to improve the poor reproductive outcomes of obese females have not been fully studied. In this study, we aimed to explore the effects and underlying mechanisms of nicotinamide mononucleotide (NMN) on oocyte quality and reproductive performance of obese mice. Materials and Methods: The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test. The expression of ovary development related genes and inflammation related genes, including Lhx8, Bmp4, Adgre1, Ccl2, TNF-α, Gal-3, Clec10a and IL-10 in ovaries and the expression of Bax and Sod1 in oocytes were detected using quantitative reverse transcription PCR (RT-qPCR). The adipose size of abdominal fat tissue was determined with haematoxylin and eosin (H&E) staining. Immunofluorescence staining was performed to measure the ROS level, spindle/ chromosome structure, mitochondrial function, actin dynamics and DNA damage of oocytes. Results: The administration of NMN restored ovarian weight and reduced the adipose size of abdominal fat tissue and ovarian inflammation in high fat diet (HFD) mice. Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice. On the long-term effect, NMN restored offspring body weight of HFD mice. Conclusion: NMN could improve the oocyte quality of HFD-induced obese mice. | INTRODUCTIONObesity has become a major global health issue with the rise of overweight. 1 Maternal obesity is closely related to poor reproductive outcomes, including early pregnancy failure, preeclampsia and neonatal conditions. 2,3 Chronic inflammation caused by obesity impacts ovarian physiology and impairs reproductive health. 4 Multiple studies have found that the oocyte quality of obese mice is impaired, due to

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Section: Discussionsupporting

confidence: 88%

Administration of nicotinamide mononucleotide improves oocyte quality of obese mice

et al. 2022

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“…We also evaluated the mitochondrial membrane potential (ΔΨm), an important indicator for mitochondrial function, by JC-1 staining [ 28 ]. Mitochondria with high ΔΨm showed red fluorescence, and with low ΔΨm showed green fluorescence (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…However, there is no better strategy to solve the damage of heat stress to animals. We previously reported that the restoration of mitochondrial function by NMN supplementation improves the quality of mouse oocytes deteriorated by maternal aging and porcine oocytes exposed to the environmental pollutant ethylene glycol butyl ether [ 23 , 28 ]. Therefore, we hypothesized that NMN is likely to ameliorate the quality of porcine oocytes under heat stress.…”

Section: Discussionmentioning

confidence: 99%

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Song

Liu

Zhou

et al. 2022

J Animal Sci Biotechnol

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Background Elevated ambient temperature-caused heat stress is a major concern for livestock production due to its negative impact on animal feed intake, growth, reproduction, and health. Particularly, the germ cells are extremely sensitive to the heat stress. However, the effective approach and strategy regarding how to protect mammalian oocytes from heat stress-induced defects have not been determined. Methods Germinal vesicle (GV) porcine oocytes were cultured at 41.5 °C for 24 h to induce heat stress, and then cultured at 38.5 °C to the specific developmental stage for subsequent analysis. Nicotinamide mononucleotide (NMN) was dissolved in water to 1 mol/L for a stock solution and further diluted with the maturation medium to the final concentrations of 10 μmol/L, 20 μmol/L, 50 μmol/L or 100 μmol/L, respectively, during heat stress. Immunostaining and fluorescence intensity quantification were applied to assess the effects of heat stress and NMN supplementation on the key processes during the oocyte meiotic maturation. Results Here, we report that NMN supplementation improves the quality of porcine oocytes under heat stress. Specifically, we found that heat stress resulted in oocyte maturation failure by disturbing the dynamics of meiotic organelles, including the cytoskeleton assembly, cortical granule distribution and mitochondrial function. In addition, heat stress induced the production of excessive reactive oxygen species (ROS) and DNA damage, leading to the occurrence of apoptosis in oocytes and subsequent embryonic development arrest. More importantly, we validated that supplementation of NMN during heat stress restored the meiotic defects during porcine oocyte maturation. Conclusions Taken together, our study documents that NMN supplementation is an effective approach to improve the quality of oocytes under heat stress by promoting both nuclear and cytoplasmic maturation.

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“…All data were normalized by GAPDH, and the comparative CT method was used to quantify the fold changes. The list of primers used was as follows (Miao et al, 2021):…”

Section: Rna Extraction and Quantitativementioning

confidence: 99%

Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function

Chen

Cui

Qiu

et al. 2021

Front. Cell Dev. Biol.

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Copper (Cu) is an essential trace element for animals, and also an important nutritional component for the normal physiology and metabolism of animal reproductive systems. An excess or lack of Cu will directly or indirectly affect animal reproductive activities. However, the effect of Cu, in particular excessive Cu, on the reproductive performance of sows has not been studied. Here, we report that excessive Cu had negative effects on oocyte maturation and organelle functions. We showed that Cu exposure perturbed porcine oocyte meiotic maturation and impaired spindle/chromosome structure, resulting in a defective spindle assembly, as well as the abnormal distribution of actin dynamics and cortical granules. In addition, single-cell transcriptome analysis identified the target effectors of Cu actions in porcine oocytes, further demonstrating that Cu exposure affects the mitochondrial distribution and function, leading to the high levels of reactive oxygen species, DNA damage, and early apoptosis of porcine oocytes. These findings demonstrate that Cu exposure causes abnormalities in the mitochondrial distribution and function, resulting in the increased oxidative stress and levels of reactive oxygen species, DNA damage, and apoptosis, ultimately leading to a decreased porcine oocyte quality.

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Nicotinamide Mononucleotide Restores the Meiotic Competency of Porcine Oocytes Exposed to Ethylene Glycol Butyl Ether

Cited by 11 publications

References 38 publications

Administration of nicotinamide mononucleotide improves oocyte quality of obese mice

Administration of nicotinamide mononucleotide improves oocyte quality of obese mice

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function

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