“… 4 These epigenetic changes increased the cardiomyocyte expression of hypertrophic genes such as Nppb and contributed to Echs1-associated cardiomyopathy in mouse models. In the present study, Cai et al 2 identified another new epigenetic mechanism of ECHS1 function in cardiac fibroblasts that modulates fibrotic gene expression and fibrosis.…”
mentioning
confidence: 58%
“…Loss-of-function mutations of ECHS1 in humans cause a range of mild to severe symptoms that often include cardiomyopathy, but the underlying mechanisms are not well understood. In this issue of JACC: Basic to Translational Science , Cai et al 2 report a new mechanism for ECHS1 in epigenetic regulation of cardiac fibroblast gene expression and cardiac fibrosis ( Figure 1 ). Figure 1 Cell Type–Specific Epigenetic Mechanisms for ECHS1 in Regulating Cardiac Gene Expression and Function In cardiomyocytes (left) , Echs1 haploinsufficiency (red arrow) results in elevated crotonyl-CoA levels and increases the expression of hypertrophic genes via histone crotonylation of H3K18 and H2BK12.…”
mentioning
confidence: 99%
“…Because Echs1 -knockout mice exhibit embryonic lethality 5 , Cai et al 2 carefully evaluated the cardiac functions of Echs1 -heterozygous ( Echs1 +/− ) mice in which the cardiac levels of both Echs1 RNA and protein are about one-half that of control. Both male and female Echs1 +/− mice showed decreased contractile function and increased heart weight, left ventricular internal dimension, and interventricular septum thickness, which were more pronounced in older mice.…”
mentioning
confidence: 99%
“…To investigate whether epigenetic mechanisms may explain these results, Cai et al 2 examined multiple histone modifications. They found that H3K9 acetylation (H3K9Ac) was specifically increased in Echs1 +/− mouse hearts.…”
mentioning
confidence: 99%
“…These results led the authors to investigate other additional mechanisms underlying the cardiac fibroblast–specific H3K9Ac changes. Analyzing Echs1-interacting proteome results from their previous study, 7 Cai et al 2 found that Echs1 physically interacted with the histone acetyltransferase p300. While other known H3K9Ac-modifying enzymes remained unchanged in primary cardiac fibroblasts from Echs1 +/− mice, decreased Echs1 increased p300 translocation into the nucleus and consequently nuclear H3K9Ac levels.…”
“… 4 These epigenetic changes increased the cardiomyocyte expression of hypertrophic genes such as Nppb and contributed to Echs1-associated cardiomyopathy in mouse models. In the present study, Cai et al 2 identified another new epigenetic mechanism of ECHS1 function in cardiac fibroblasts that modulates fibrotic gene expression and fibrosis.…”
mentioning
confidence: 58%
“…Loss-of-function mutations of ECHS1 in humans cause a range of mild to severe symptoms that often include cardiomyopathy, but the underlying mechanisms are not well understood. In this issue of JACC: Basic to Translational Science , Cai et al 2 report a new mechanism for ECHS1 in epigenetic regulation of cardiac fibroblast gene expression and cardiac fibrosis ( Figure 1 ). Figure 1 Cell Type–Specific Epigenetic Mechanisms for ECHS1 in Regulating Cardiac Gene Expression and Function In cardiomyocytes (left) , Echs1 haploinsufficiency (red arrow) results in elevated crotonyl-CoA levels and increases the expression of hypertrophic genes via histone crotonylation of H3K18 and H2BK12.…”
mentioning
confidence: 99%
“…Because Echs1 -knockout mice exhibit embryonic lethality 5 , Cai et al 2 carefully evaluated the cardiac functions of Echs1 -heterozygous ( Echs1 +/− ) mice in which the cardiac levels of both Echs1 RNA and protein are about one-half that of control. Both male and female Echs1 +/− mice showed decreased contractile function and increased heart weight, left ventricular internal dimension, and interventricular septum thickness, which were more pronounced in older mice.…”
mentioning
confidence: 99%
“…To investigate whether epigenetic mechanisms may explain these results, Cai et al 2 examined multiple histone modifications. They found that H3K9 acetylation (H3K9Ac) was specifically increased in Echs1 +/− mouse hearts.…”
mentioning
confidence: 99%
“…These results led the authors to investigate other additional mechanisms underlying the cardiac fibroblast–specific H3K9Ac changes. Analyzing Echs1-interacting proteome results from their previous study, 7 Cai et al 2 found that Echs1 physically interacted with the histone acetyltransferase p300. While other known H3K9Ac-modifying enzymes remained unchanged in primary cardiac fibroblasts from Echs1 +/− mice, decreased Echs1 increased p300 translocation into the nucleus and consequently nuclear H3K9Ac levels.…”
The great potential of zinc oxide nanoparticles (ZnO NPs) for biomedical applications is attributed to their physicochemical properties. In this work, pure and Ag and Ce dual-doped ZnO NPs were synthesized through a facile and green route to examine their cytotoxicity in breast cancer and normal cells. The initial preparation of dual-doped nanoparticles was completed by the usage of
taranjabin
. The synthesis of Ag and Ce dual-doped ZnO NPs was started with preparing the Ce:Ag ratios of 1:1, 1:2, and 1:4. The cytotoxicity effects of synthesized nanoparticles against breast normal cells (MCF-10A) and breast cancer cells (MDA-MB-231) were examined. The hexagonal structure of synthesized nanoparticles was observed through the results of X-ray diffraction (XRD). Scanning electron microscopy (SEM) images exhibited the spherical shape and smooth surfaces of prepared particles along with the homogeneous distribution of Ag and Ce in ZnO with high-quality lattice fringes without any distortions. According to the cytotoxic results, the effects of Ag/Ce dual-doped ZnO NPs on breast cancer (MDA-MB-231) cells were significantly more than of pure ZnO NPs, while dual-doped and pure nanoparticles remained indifferent towards breast normal (MCF-10A) cells. In addition, we investigated the antimicrobial activity against harmful bacteria.
Graphical Abstract
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
