Nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase activity in the brain of a cichlid fish, with remarkable findings in the entopeduncular nucleus: a histochemical study

Jadhao, Arun G.; Malz, Cordula R.

doi:10.1016/j.jchemneu.2003.12.001

Cited by 16 publications

(19 citation statements)

References 43 publications

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“…In addition, the large inferior hypothalamic lobes of gars also show a prominent population of NOS-ir cells located in the nucleus of the lateral recess. This peculiar brain structure in the hypothalamus of teleosts shows similar nitrergic cells in the nucleus of the lateral recess, but also in the central and diffuse nuclei of the inferior hypothalamic lobes [Arévalo et al, 1995;Brüning et al, 1995;Bordieri et al, 2003;Ando et al, 2004;Giráldez-Pérez et al, 2008], with the exceptions of C. batrachus and Haplochromis burtoni [Jadhao and Malz, 2004;Gaikwad et al, 2009]. The inferior hypothalamic lobes of teleosts are related to feeding and aggressive biting [Demski and Knigge, 1971], receiving indirect visual information from the pretectum [Wullimann and Meyer, 1990;Wullimann et al, 1991], taste information from secondary gustatory centers [Finger and Kanwal, 1992;Lamb and Caprio, 1993;Folgueira et al, 2003] and general visceral centers [Yoshimoto and Yamamoto, 2010;Kato et al, 2011] to produce a brainstem motor response that implicates activation of the trigeminal and facial motor nuclei.…”

Section: Forebrainmentioning

confidence: 84%

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

et al. 2017

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The study of the nitrergic system, formed by the networks of neurons containing the enzyme nitric oxide synthase (NOS), has been extremely useful in unraveling neuroanatomical features of the organization of the central nervous system of vertebrates. Thus, data are available for representatives of most vertebrate classes and, in particular, several studies have detailed the organization of this system in teleosts. In contrast, no information is available regarding this neurotransmission system in the brains of holosteans, an early diverged and poorly understood group of actinopterygian fishes, currently considered a sister group of teleosts that contains only 8 species. The present study provides the first detailed information on the distribution of nitrergic cell bodies and fibers in 2 holostean species of the genus Lepisosteus, the spotted gar L. oculatus and the Florida gar L. platyrhincus. NOS immunohistochemistry and the NADPH diaphorase (NADPH-d) histochemical reaction were used, and both techniques yielded identical results, with the exception of the primary olfactory and terminal nerve fibers, which only labeled for NADPH-d exclusively in L. oculatus. Double immunohistochemistry was conducted for the simultaneous detection of NOS with tyrosine hydroxylase, choline acetyltransferase, calbindin, calretinin, and serotonin to accurately establish the localization of the nitrergic neurons and fibers in the brain of holosteans, the neuroanatomy of which has been mostly neglected, and to assess possible interactions between these neuroactive substances. Distinct groups of nitrergic cells were located in subpallial areas, the basal hypothalamus, posterior tubercle, optic tectum and mesencephalic tegmentum, reticular formation, solitary tract nucleus, spinal cord, and amacrine cells in the retina. In addition, low numbers of nitrergic cells were observed in the pallium, suprachiasmatic nucleus, prethalamic and thalamic areas, torus lateralis and torus semicircularis, cerebellar and laterodorsal tegmental nuclei, and the ventral octavolateral area. Comparison of these results with those from other classes of vertebrates, and including a segmental analysis to correlate cell populations, reveals that the pattern of the nitrergic system in holosteans is very close to that in ancestral actinopterygian fishes and highlights conserved and derived traits.

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Section: Forebrainmentioning

confidence: 84%

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

et al. 2017

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“…So far, to our knowledge, NADPH-d activity has been reported only in the MC of adult swordtail fish and goldfish (Bell et al 1997) and our results on adult are in agreement with these investigations. However, other studies on the NADPH-d localization in the brain of tench, Tinca tinca (Arévalo et al 1995), goldfish, Carassius auratus (Brüning et al 1995), cichlid fish, Haplochromis burtoni (Jadhao and Malz 2004) and several catfish species (Jadhao et al 1999) do not report the presence of diaphorase in the MC. Therefore, it seems that in the larval and adult fish brains NADPH-d localization in the MC is highly species-specific.…”

Section: Nadph-d and Nnos In The Mauthner Neuronsmentioning

confidence: 95%

“…In view of such special characteristics, this technique was used to trace the central projections in the CNS of lungfish (Schober et al 1994a) and also for the demonstration of NOS-expressing neurons in the CNS of larval lamprey (Schober et al 1994a). Many workers have shown neuronal diaphorase in the CNS of fish using NADPH-d histochemistry (Arévalo et al 1995;Brüning et al 1995;Crespo et al 1998;Bell et al 1997;Jadhao et al 1999;Jadhao and Malz 2004). However, it is difficult to understand from the existing studies on NADPH-d in the fish brain whether the activity was absent from MC or whether MC were simply not examined in previous investigations, because many studies do not report MC within their results.…”

Section: General Remarksmentioning

confidence: 99%

The ontogeny of Mauthner cells in the brain of Labeo rohita as revealed by NADPH-d and nNOS immunohistochemistry

2010

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Nitric oxide (NO) is well demonstrated to act as a neuronal messenger in neurotransmission in vertebrate animals. We are for the first time reporting nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and neuronal nitric oxide synthase (nNOS) in the Mauthner cells (MC) of the carp, Labeo rohita brain during post-embryonic development. The NADPH-d and nNOS-positive MC were seen intensely labeled in all young stages on either side of the ventricle in the hindbrain. In adult, these cells were moderately stained with NADPH-d but not with nNOS. Further, the morphometeric analysis of the staining intensities showed the decrease in the optical densities of the MC as the development progresses. The gradual increase in cell diameter was evident from hatchling to adult (18-22 μm to 98-106 μm), while the nuclear diameter remained nearly unchanged (10-12 μm to 18-20 μm). In fishes, MC are known to be involved in the control of startle response. The NADPH-d enzymatic activity seen in the MC of early development may play an important role to activate the startle response to fast escape from the predators and unfavorable environmental conditions to survive during early phase of life. As nNOS is absent in the adult MC, we assume that the adult MC express a different splice variant of nNOS which is detected by NADPH-d, but not by the currently used antibody. Hence, the presence of nNOS (enzyme required for the synthesis of NO) in the MC suggests the involvement of NO in neurotransmission to generate the startle response rapidly. Furthermore, we also suggest that NO may be involved in other aspects such as pathway finding, neuronal cell migration, signaling and refinement during the development of the brain of L. rohita.

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“…NE seems to be an important candidate for NPY innervation of a considerable part of the telencephalon. These neurons are reported to have extensive fiber terminals in the dorsal and lateral regions of the telencephalon of another cichlid fish, Haplochromis burtoni (Jadhao and Malz, 2004). Pickavance et al (1992) observed that the cells of Vl as well as NE colocalize NPY with somatostatin and suggested the possibility that these neurons have extensive projections to the dorsal telencephalon.…”

Section: Response Of the Npy-ir System To Castration And Testosteronementioning

confidence: 99%

Neuropeptide Y in the forebrain of the adult male cichlid fish Oreochromis mossambicus: Distribution, effects of castration and testosterone replacement

Sakharkar

Singru

Sarkar

et al. 2005

J of Comparative Neurology

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We studied the organization of the neuropeptide Y (NPY)-immunoreactive system in the forebrain of adult male cichlid fish Oreochromis mossambicus and its response to castration and testosterone replacement by using morphometric methods. Immunoreactivity for NPY was widely distributed in the forebrain, and the pattern generally resembled that in other teleosts. Whereas immunoreactivity was conspicuous in the ganglia of nervus terminalis (NT; or nucleus olfactoretinalis), a weak reaction was detected in some granule cells in the olfactory bulb and in the cells of area ventralis telencephali pars lateralis (Vl). Moderately to intensely immunoreactive cells were distinctly seen in the nucleus entopeduncularis (NE), nucleus preopticus (NPO), nucleus lateralis tuberis (NLT), paraventricular organ (PVO), and midbrain tegmentum (MT). NPY fibers were widely distributed in the forebrain. Castration for 10/15 days resulted in a drastic loss of immunoreactivity in the cells of NE (P<0.001) and a significant decrease (P<0.01) in their cell nuclear size. However, cell nuclei of the NT neurons showed a significant increase in size. A highly significant reduction in the NPY-immunoreactive fiber density (P<0.001) was observed in several areas of the forebrain. Although testosterone replacement reversed these changes, fibers in some areas showed supranormal responses. Immunoreactive cells in Vl, NPO, NLT, PVO, and MT and fiber density in some other areas did not respond to castration. We suggest that the NPY-immunoreactive elements that respond to castration and testosterone replacement may serve as the substrate for processing the positive feedback action of the steroid hormone.

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Nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase activity in the brain of a cichlid fish, with remarkable findings in the entopeduncular nucleus: a histochemical study

Cited by 16 publications

References 43 publications

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

The ontogeny of Mauthner cells in the brain of Labeo rohita as revealed by NADPH-d and nNOS immunohistochemistry

Neuropeptide Y in the forebrain of the adult male cichlid fish Oreochromis mossambicus: Distribution, effects of castration and testosterone replacement

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