Niclosamide inhibits leaf blight caused by Xanthomonas oryzae in rice

Kim, Sung-Il; Song, Jong Tae; Jeong, Jin‐Yong; Seo, Hak Soo

doi:10.1038/srep21209

Cited by 22 publications

(15 citation statements)

References 54 publications

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“…The fully expanded 4th and 5th leaves staged rice seedlings were inoculated by Xoo bacterial suspensions (OD 600 = 0.5) by leaf-tip-clipping method ( Kauffman et al, 1973 ). The rice plants were sprayed with 10 µg/ml niclosamide at 4-day intervals ( Kim et al, 2016b ). Plants inoculated with the 0.02% Tween-20 solution were used as mock-inoculated controls.…”

Section: Methodsmentioning

confidence: 99%

“…Niclosamide has been successfully used as an orally bioavailable chlorinated salicylanilide, with anthelmintic and potential antineoplastic activity ( Imperi et al, 2013 ). Niclosamide is a very famous potent drug to cure gastrointestinal tapeworm infections in both humans and animals since 1960s ( Weinbach and Garbus, 1969 ; Kim et al, 2016b ). An extensive study in the animal system shows that “niclosamide has effective antiviral activity against the severe acute respiratory syndrome virus, anti-anthrax toxin properties, and anti-neoplastic activity.…”

Section: Introductionmentioning

confidence: 99%

“…These findings clearly demonstrate that niclosamide has various curative effects on humans and animals. While searching for a master regulator that effectively functions to protect both animals and plants from infectious diseases recently Kim et al (2016b) showed that “niclosamide can also inhibit the growth of bacterial plant pathogen Xoo, and it can move long distances from the site of local application to distant rice tissues. Niclosamide also increased the levels of salicylate and induced the expression of defense-related genes thereby suppressing the Xoo-induced leaf wilting.…”

Section: Introductionmentioning

confidence: 99%

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Niclosamide Blocks Rice Leaf Blight by Inhibiting Biofilm Formation of Xanthomonas oryzae

2018

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Rice (Oryza sativa) is the leading source of nutrition for more than half of the world’s population, and by far it is the most important commercial food crop. But, its growth and production are significantly hampered by the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) which causes leaf blight disease. Earlier studies have reported the antibacterial ability of FDA-approved niclosamide drug against Xoo. However, the underlying mechanism by which niclosamide blocks the growth of Xoo remained elusive. In the present study, by employing the microbiological, microscopical, molecular, bioinformatics and analytical tools we found that niclosamide can directly inhibit the growth of the Xoo by hampering the biofilm formation and the production of xanthomonadin and exopolysaccharide substances (EPS) required for relentless growth and virulence of Xoo. Interestingly, niclosamide was found to specifically suppress the growth of Xoo without affecting other bacteria like Escherichia coli. Our electron microscopic observations disclosed that niclosamide disrupts the membrane permeability of Xoo and causes the release of intracellular components. Similarly, the molecular docking analysis disclosed the molecular interaction of niclosamide with the biofilm, virulence and quorum sensing related proteins, which was further substantiated by relative gene expression analysis where niclosamide was found to significantly downregulate the expression of these key regulatory genes. In addition, considerable changes in chemical structures were detected by Fourier Transform Infrared Spectroscopy (FTIR) in response to niclosamide treatment. Overall, our findings advocate the utilization of niclosamide as a safe and potent alternative antibacterial compound to control bacterial blight disease in rice.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Niclosamide Blocks Rice Leaf Blight by Inhibiting Biofilm Formation of Xanthomonas oryzae

2018

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“…oryzae menjadi salah satu penyebab kehilangan hasil di negara-negara penghasil padi khususnya di Asia (Ezuka dan Kaku 2000). Berbagai upaya pengendalian penyakit ini telah dilakukan baik secara biotik (Putra dan Giyanto 2014; Krishanti et al 2015) maupun secara abiotik (Mustafa et al 2013;Kim et al 2015), tetapi hingga saat ini penyakit HDB masih menjadi kendala utama produksi padi di daerah tropis maupun subtropis. Berdasarkan hasil survei Sudir dan Yuliani (2016) pada 10 provinsi di Indonesia ditemukan 3 patotipe yang dominan, yaitu patotipe III (30%), IV (36%), dan VIII (34%).…”

Section: Pendahuluanunclassified

Asam Salisilat sebagai Penginduksi Ketahanan Tanaman Padi terhadap Penyakit Hawar Daun Bakteri

Leiwakabessy¹,

Sinaga²,

Mutaqin³

et al. 2018

J Fitopatol Indones

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The use of salicylic acid as resistance inducers agents in several plants species was well known. Salicylic acid has been believed to play an important role in inducing resistance against some pathogen. This research was aimed to study the role and effectiveness of salicylic acid as inducers for the resistance of some rice variety against bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. The experiment was arranged in completely randomized factorial design with three factors i.e. rice variety, salicylic acid, and pathotypes, each treatment was replicated three times. The results showed that salicylic acid could prolong the latent period of X. oryzae pv. oryzae pathotypes IV and VIII in paddy rice varieties Conde and Ciherang, induce the resistance of rice varieties as well as reducing the rate of infection of the two mentioned pathotypes. Salicylic acid was also able to induce the resistance of Conde and Ciherang varieties in order to suppress area under disease progress curve (AUDPC) of X. oryzae pv. oryzae pathotypes IV and VIII.

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“…However, enhancing genetic resistance has proven to be the most effective method of controlling BLB disease. A total of 38 BLB resistance genes (R genes), designated as Xa1 to Xa38, have been identified in rice (Khan et al, 2014;Kim et al, 2016). These R genes evoke a strong, normally race-specific, resistance that results in very short lesions and reduction of susceptibility.…”

Section: Introductionmentioning

confidence: 99%

Preliminary genome-wide association mapping of rice bacterial leaf blight resistance loci using major Korean races of Xoo (Xanthomonas oryzae)

Ali¹,

Yoon-Hyun²,

Noh³

et al. 2017

Plant Omics

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Bacterial leaf blight (BLB), caused by X. oryzae pv. oryzae (Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 96 accessions was used in the genomewide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. The results of the bioassay using a selected set of 96 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. The genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than 5 (K1 and K2) and more than 4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. These SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice cultivars.

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Niclosamide inhibits leaf blight caused by Xanthomonas oryzae in rice

Cited by 22 publications

References 54 publications

Niclosamide Blocks Rice Leaf Blight by Inhibiting Biofilm Formation of Xanthomonas oryzae

Niclosamide Blocks Rice Leaf Blight by Inhibiting Biofilm Formation of Xanthomonas oryzae

Asam Salisilat sebagai Penginduksi Ketahanan Tanaman Padi terhadap Penyakit Hawar Daun Bakteri

Preliminary genome-wide association mapping of rice bacterial leaf blight resistance loci using major Korean races of Xoo (Xanthomonas oryzae)

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