Nickel Toxicity, Regulation, and Resistance in Bacteria

Macomber, Lee; Hausinger, Robert P.

doi:10.1002/9781119004813.ch110

Cited by 9 publications

(5 citation statements)

References 134 publications

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“…Lower threshold concentrations for toxicity were previously observed for Ni when compared with Zn under the same experimental conditions (Hao et al 1994;Poulson et al 1997), again potentially due to the tendency for Zn to form Zn sulfides over Ni in mixed metal solutions, as predicted by PHREEQC. Divalent Ni competes catabolically and allosterically with Zn and Fe and therefore interferes with metalloenzyme activities, as well as generates reactive oxygen (Macomber and Hausinger 2016). Therefore, the observed inhibition of SCN − biodegradation in Ni-amended experiments may be attributed to Ni 2+ species that comprised a larger proportion of total Ni at higher amendment concentrations, as modeled.…”

Section: Discussionmentioning

confidence: 93%

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

Shafiei

Watts

Pajank

et al. 2020

Appl Microbiol Biotechnol

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Bioremediation systems represent an environmentally sustainable approach to degrading industrially generated thiocyanate (SCN−), with low energy demand and operational costs and high efficiency and substrate specificity. However, heavy metals present in mine tailings effluent may hamper process efficiency by poisoning thiocyanate-degrading microbial consortia. Here, we experimentally tested the tolerance of an autotrophic SCN−-degrading bacterial consortium enriched from gold mine tailings for Zn, Cu, Ni, Cr, and As. All of the selected metals inhibited SCN− biodegradation to different extents, depending on concentration. At pH of 7.8 and 30 °C, complete inhibition of SCN− biodegradation by Zn, Cu, Ni, and Cr occurred at 20, 5, 10, and 6 mg L−1, respectively. Lower concentrations of these metals decreased the rate of SCN− biodegradation, with relatively long lag times. Interestingly, the microbial consortium tolerated As even at 500 mg L−1, although both the rate and extent of SCN− biodegradation were affected. Potentially, the observed As tolerance could be explained by the origin of our microbial consortium in tailings derived from As-enriched gold ore (arsenopyrite). This study highlights the importance of considering metal co-contamination in bioreactor design and operation for SCN− bioremediation at mine sites. Key points • Both the efficiency and rate of SCN−biodegradation were inhibited by heavy metals, to different degrees depending on type and concentration of metal. • The autotrophic microbial consortium was capable of tolerating high concentrations of As, potential having adapted to higher As levels derived from the tailings source.

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Section: Discussionmentioning

confidence: 93%

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

Shafiei

Watts

Pajank

et al. 2020

Appl Microbiol Biotechnol

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“…Copper displays a high affinity for metalloproteins, and if equivalent quantities of all divalent metals were present, proteins would probably all bind copper [ 35 ]. The decrease in zinc content can also be explained by the inhibition of zinc metalloenzymes by nickel [ 36 ]. The reduction of strontium content can also be explained by its replacement with nickel ions [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Assessment of Metal Accumulation by Arthrospira platensis and Its Adaptation to Iterative Action of Nickel Mono- and Polymetallic Synthetic Effluents

et al. 2022

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Cyanobacteria-mediated wastewater remediation is an economical, efficient, and eco-friendly technology. The present work deals with the bioaccumulation performance of Arthrospira platensis (Spirulina) grown for four cycles in a medium containing nickel mono- and polymetallic synthetic effluents. The metal uptake by spirulina biomass was evaluated using neutron activation analysis. The effects of effluents on biomass production, protein, and phycobiliprotein content were assessed. Metal accumulation in the biomass depended on the effluent composition and metal ion concentrations. Nickel accumulation in the biomass was directly proportional to its concentration in effluents, and maximum uptake (1310 mg/kg) was attained in the Ni/Cr/Fe system. In the same system, biomass accumulated 110 times more chromium and 4.7 times more iron than control. The highest accumulation of copper (2870 mg/kg) was achieved in the Ni/Cu/Zn/Mo system and zinc (1860 mg/kg)—in the Ni/Cu/Zn/Sr system. In biomass grown in the media loaded with nickel and also chromium, iron, copper, strontium, zinc, and molybdenum, a decrease in productivity (on average by 10%) during the first cycle of cultivation and moderate reduction of protein content (by 15–27%) was observed. The presence of metals in the cultivation media inhibited phycobiliprotein synthesis, especially of phycocyanin, and promoted the synthesis of allophycocyanin. The maximum reduction of phycocyanin content was 77%, and the increase of allophycocyanin content—by 45%. Arthrospira platensis may be deemed as bioremediation of nickel-polluted wastewaters of complex composition.

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“…Redox‐active metals like Ni promote oxidative damage as they can exist in the (II) and (I) states; Pd(II) can possibly act as a ‘surrogate’ for Ni(II) (they are in the same group of the periodic table) but not substitute functionally. Some similarities in biochemical ‘processing’ of Ni(II) and Pd(II) might be expected, e.g.…”

Section: Discussionmentioning

confidence: 99%

Probing the viability of palladium‐challenged bacterial cells using flow cytometry

Omajali

Mikheenko²,

Overton

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND The ability of bacterial cells to retain membrane integrity and membrane potential when challenged with palladium (II) solution has not being examined previously, which would provide a platform towards the bio‐fabrication of a multifunctional tandem bio‐nanoparticles. This study investigates the use of flow cytometry coupled with fluorescent probes to determine membrane integrity and membrane potential of cells of Desulfovibrio desulfuricans and Bacillus benzeovorans challenged with 1 mmol L−1 of sodium tetrachloropalladate (II) (Na2PdCl4) solution at pH 2 followed by reduction of palladium (II) (Pd(II)) with formate to give 1 wt% loading of Pd(0) on the cells. RESULTS Fluorescently labelled active bacterial cells retained over 80% of membrane potential when challenged with Pd(II) solutions except for Bacillus benzeovorans (Bb) with about 32% retention. Cell viability was also seen to be variable and strain‐dependent while dead cells lack any membrane integrity. Since esterase activity is energy independent and unable to confirm the membrane potential of the bacterial cells, the dye 3,3′‐dihexyloxacarbocyanine iodide [DiO6(3)] was used to determine and confirm the membrane potential of the bacterial cells. CONCLUSION The results revealed that since fluorescently labelled bacterial cells containing Pd(0) can retain metabolic activity when analysed with flow cytometry, it provides the potential for combining chemical catalysis with biochemical activity in reactions that require metabolic synergy. © 2018 Society of Chemical Industry

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Nickel Toxicity, Regulation, and Resistance in Bacteria

Cited by 9 publications

References 134 publications

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings

Assessment of Metal Accumulation by Arthrospira platensis and Its Adaptation to Iterative Action of Nickel Mono- and Polymetallic Synthetic Effluents

Probing the viability of palladium‐challenged bacterial cells using flow cytometry

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