Nickel oxide nanoparticles induce genotoxicity and cellular alterations in the ground beetleBlaps polycresta(Coleoptera: Tenebrionidae)

Abstract

Nickel nanoparticles (Ni-NPs) have advantageous applications in the industry; however, little is known of their adverse effects on biological tissues. In the present study, the ground beetle Blaps polycresta was employed as a sensitive indicator for nickel oxide nanoparticles (NiO-NPs) toxicity. Adult male beetles were injected with six dose levels of NiO-NPs (0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 mg/g body weight). Mortality was reported daily over 30 days under laboratory conditions to establish an LD50. Nickel was detected in the testicular tissues of the beetles using X-ray analysis and transmission electronic microscopy. Beetles treated with the sublethal dose of 0.02 mg/g were selected to observe molecular, cellular, and subcellular changes. Gene transcripts of HSP70, HSP90, and MT1 were found to be increased >2.5-, 1.5-, and 2-fold, respectively, in the treated group compared with the controls. Decreased gene expression of AcPC01, AcPC02, and AcPC04 (≤1.5-, ≤2-, and < 2.5-fold, respectively, vs. controls) also were reported in the treated group. Under light microscopy, various structural changes were observed in the testicular tissues of the treated beetles. Ultrastructure observations using scanning and transmission electron microscopy showed severe damage to the subcellular organelles as well as deformities of the heads and flagella of the spermatozoa. Therefore, the present study postulated the impact of NiO-NPs in an ecological model.

Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model

“…It is well known that heat shock proteins (HSPs) play a paramount role in insects and mammals to maintain cellular homeostasis [ 46 ]. In our previous work, we found a noticeable upregulation of specific protective proteins, such as HSPs, as a defense strategy to govern oxidative stress [ 47 ]. Our findings demonstrate that the expressions of HSP70 and HSP90 were highly upregulated in the Al 2 O 3 NPs-exposed animals compared to the controls.…”

“…To provide more evidence on the protective influence of PAE in relation to attenuating the hazardous episodes provoked by the toxicity of the testicular tissues with Al 2 O 3 NPs, we inspected the histological and ultrastructural characteristics of the testes compared to the testicular tissues exposed to a single application of Al 2 O 3 NPs and those from control locusts. Prior investigations have evidenced that treatment with either Ag NPs or NiO NPs incited various anomalies in the testicular tissue of beetles, including deformed spermatogenic elements and agglutinated spermatids, in addition to axonemal and mitochondrial deformations [ 4 , 47 , 57 ]. Furthermore, Wang et al [ 58 ] reported that exposure to nanoparticles such as TiO 2 , gold alloys, nickel NPs, and Ag NPs had serious effects on the male reproductive system.…”

“…In this study, histological, SEM, and TEM analyses demonstrated different aberrations in testicular tissues from locusts treated with Al 2 O 3 NPs, particularly a substantial degradation of the mitochondria. The disintegration of mitochondrial structures and their derivatives conceivably disrupts the ATP supply for sperm motility, leading to infertility [ 4 , 47 ]. These observations imply the alarming toxicity of Al 2 O 3 NPs connected with bioburden toward cells, even if they are administered in low doses.…”

“…These observations imply the alarming toxicity of Al 2 O 3 NPs connected with bioburden toward cells, even if they are administered in low doses. Kheirallah et al [ 47 ] elucidated that the secondary fusion of the spermatogenic elements during mitosis could be the underlying cause behind the appearance of bi- and tetra-flagellated sperms. In contrast to these findings, the testicular tissues of the group pretreated with PAE showed the ameliorative capacity of PAE and its role in protecting testicular tissues, with no manifestations of sperm cell agglutination.…”

Physiological responses (HSH70, Mt), Oxidative stress, toxicity impacts, and risk assessment of the biomarker (Enochrus tenuicosta) to heavy metals contamination along the Red Sea coasts- Egypt.

“…Insects are growing commonplace worldwide and have been recognized as possible bioindicators of water quality and ecosystems. Due to their accurate sensory system, which enables them to qualify environmental conditions in specific situations and also quantify environmental damages caused to the environment, they refer to changes in the environment because they are very sensitive to pollutants and other types of environmental stress (15,16). To assess the water quality, physicochemical characteristics, and insects as indicators may be used.…”