Nickel contamination analysis at cost-effective silver printed paper-based electrodes based on carbon black dimethylglyoxime ink as electrode modifier

Pokpas, Keagan; Jahed, Nazeem; Bezuidenhout, Petrone; Smith, Suzanne; Land, Kevin; Iwuoha, Emmanuel I.

doi:10.5599/jese.1173

Cited by 6 publications

(4 citation statements)

References 41 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method obtained a resolution of 600 dpi, which was less than that reported in a previous study. Pokpas et al [119,120] studied two approaches of carbon-nanomaterial-modified Ag nanoparticle electrodes for metal analysis in water.…”

Section: Inkjet Printingmentioning

confidence: 99%

See 1 more Smart Citation

Advances in paper-based electrochemical immunosensors: review of fabrication strategies and biomedical applications

du Plooy,

Jahed,

Iwuoha

et al. 2023

R. Soc. open sci.

Self Cite

View full text Add to dashboard Cite

Cellulose paper-based sensing devices have shown promise in addressing the accuracy, sensitivity, selectivity, analysis time and cost of current disease diagnostic tools owing to their excellent physical and physiochemical properties, high surface-area-to-volume ratio, strong adsorption capabilities, ease of chemical functionalization for immobilization, biodegradability, biocompatibility and liquid transport by simple capillary action. This review provides a comprehensive overview of recent advancements in the field of electrochemical immunosensing for various diseases, particularly in underdeveloped regions and globally. It highlights the significant progress in fabrication techniques, fluid control, signal transduction and paper substrates, shedding light on their respective advantages and disadvantages. The primary objective of this review article is to compile recent advances in the field of electrochemical immunosensing for the early detection of diseases prevalent in underdeveloped regions and globally, including cancer biomarkers, bacteria, proteins and viruses. Herein, the critical need for new, simplistic early detection strategies to combat future disease outbreaks and prevent global pandemics is addressed. Moreover, recent advancements in fabrication techniques, including lithography, printing and electrodeposition as well as device orientation, substrate type and electrode modification, have highlighted their potential for enhancing sensitivity and accuracy.

show abstract

Section: Inkjet Printingmentioning

confidence: 99%

“…Pokpas et al . [ 119 , 120 ] studied two approaches of carbon-nanomaterial-modified Ag nanoparticle electrodes for metal analysis in water.…”

Section: Electrode Modificationmentioning

confidence: 99%

Advances in paper-based electrochemical immunosensors: review of fabrication strategies and biomedical applications

du Plooy,

Jahed,

Iwuoha

et al. 2023

R. Soc. open sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Carbon nanomaterials have gained significant attention in recent years, specifically in energy, biomedical, and sensing applications, driven by the desire to reduce costs and adopt more environmentally friendly practices in various industries. In electroanalytical applications, they have proven to be particularly valuable to improving electrode conductivity and active surface area [23,24]. This is attributed to their chemical inertness, wide operational potential range, versatility in different types of analyses, and outstanding electrochemical properties [25].…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanostructured Immunosensing of Anti-SARS-CoV-2 S-Protein Antibodies

du Plooy,

Kock,

Jahed

et al. 2023

Molecules

View full text Add to dashboard Cite

The rampant spread and death rate of the recent coronavirus pandemic related to the SARS-CoV-2 respiratory virus have underscored the critical need for affordable, portable virus diagnostics, particularly in resource-limited settings. Moreover, efficient and timely monitoring of vaccine efficacy is needed to prevent future widespread infections. Electrochemical immunosensing poses an effective alternative to conventional molecular spectroscopic approaches, offering rapid, cost-effective, sensitive, and portable electroanalysis of disease biomarkers and antibodies; however, efforts to improve binding efficiency and sensitivity are still being investigated. Graphene quantum dots (GQDs) in particular have shown promise in improving device sensitivity. This study reports the development of a GQD-functionalized point-of-contamination device leveraging the selective interactions between SARS-CoV-2-specific Spike (S) Protein receptor binding domain (RBD) antigens and IgG anti-SARS-CoV-2-specific S-protein antibodies at screen-printed carbon electrode (SPCE) surfaces. The immunocomplexes formed at the GQD surfaces result in the interruption of the redox reactions that take place in the presence of a redox probe, decreasing the current response. Increased active surface area, conductivity, and binding via EDC/NHS chemistry were achieved due to the nanomaterial inclusion, with 5 nm, blue luminescent GQDs offering the best results. GQD concentration, EDC/NHS ratio, and RBD S-protein incubation time and concentration were optimized for the biosensor, and inter- and intra-screen-printed carbon electrode detection was investigated by calibration studies on multiple and single electrodes. The single electrode used for the entire calibration provided the best results. The label-free immunosensor was able to selectively detect anti-SARS-CoV-2 IgG antibodies between 0.5 and 100 ng/mL in the presence of IgM and other coronavirus antibodies with an excellent regression of 0.9599. A LOD of 2.028 ng/mL was found, offering comparable findings to the literature-reported values. The detection sensitivity of the sensor is further compared to non-specific IgM antibodies. The developed GQD immunosensor was compared to other low-oxygen content carbon nanomaterials, namely (i) carbon quantum dot (CQD), (ii) electrochemically reduced graphene oxide, and (iii) carbon black-functionalized devices. The findings suggest that improved electron transfer kinetics and increased active surface area of the CNs, along with surface oxygen content, aid in the detection of anti-SARS-CoV-2 IgG antibodies. The novel immunosensor suggests a possible application toward monitoring of IgG antibody production in SARS-CoV-2-vaccinated patients to study immune responses, vaccine efficacy, and lifetime to meet the demands for POC analysis in resource-limited settings.

show abstract

“…Nafion/TiO2 for the selective determination of olopatadine [10] and with Cu complexes for the simultaneous determination of dopamine and uric acid [11]. Modified carbon paste electrodes were also used in the determination of nickel contamination [12] and glutathione [13].…”

mentioning

confidence: 99%