Nibrin expression in oral squamous cell carcinoma: association with clinicopathological parameters

Dave, Jigna H.; Vora, Hemangini H.; Trivedi, Trupti; Ghosh, Nandita

doi:10.20517/2394-4722.2015.82

Cited by 7 publications

(14 citation statements)

References 32 publications

(41 reference statements)

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“…The scoring system has already been published in our previous literature . All sections were scored independently by two independent researchers in a blinded fashion.…”

Section: Methodsmentioning

confidence: 99%

Mediator of DNA damage checkpoint protein 1 (MDC1) as a prognostic marker for patients with oral squamous cell carcinoma

Dave

Vora

Ghosh

et al. 2017

J Oral Pathology Medicine

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“…The scoring system has already been published in our previous literature . All sections were scored independently by two independent researchers in a blinded fashion.…”

Section: Methodsmentioning

confidence: 99%

Mediator of DNA damage checkpoint protein 1 (MDC1) as a prognostic marker for patients with oral squamous cell carcinoma

Dave

Vora

Ghosh

et al. 2017

J Oral Pathology Medicine

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“…Nibrin protein plays an important central role in double-stranded breakage repair pathway; oncoproteins are responsible for the activation of mutated nibrin which further induces the epithelial mesenchymal transition increasing the invasiveness and metastasis in the later stages of head and neck cancer [29]. Another study by Jigna H Dave et al in 2016 also showed similar findings as significant inverse-correlation of Nibrin over expression was associated with tumor size and tumor stage [14].…”

Section: Discussionmentioning

confidence: 80%

“…Some recent studies have reported abbarent NBS1expression in proliferating oral tissues suggesting a potential to predict carcinogenic process early on [14,15]. However, the status of NBS1 in subset of oral cancer from our population remains unknown.…”

Section: Introductionmentioning

confidence: 82%

“…Nibrin is a 754 amino acid containing DNA breakdown repairing protein formed by NBN gene. It is identified as an important tri-meric member of NBS1/hMre11/RAD50 (N/M/R), referred as MRN (DNA) DSB break repair complex [14]. Whenever the DNA has been damaged this trimeric complex identifies DNA breakdown and forms nuclear foci at these DSB sites.…”

Section: Discussionmentioning

confidence: 99%

“…All positive cases were scored by employing; Hscoring criteria to determine the nuclear expression of Nibrin and interpretated as; Negative (0), Weak (1+), Moderate (2++); more than 25% in tumor cells while strong Intensity (3+++) reflects strong staining in at least 50% of tumor cells. To authenticate the reaction chronic cholecystitis specimen were used as positive IHC control [14].…”

Section: Methodsmentioning

confidence: 99%

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Immunohistochemical Expression of Nibrin in Epithelial Dysplasia and OSCC: A Cross-Sectional Study

Abidi

Hosein

Butt

et al. 2021

JAMMR

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Inherited defect in DNA repair capability is the fundamental problem causing mutations to be passed on to new generation of cells leading to cancer. The NBS1/Nibrin/p95 belongs to the family of the DNA double-strand break repair complex (hMre11 complex) which is a transcript of the mutated NBS gene, located on human chromosome 8q21 The protein complex containing nibrin binds to the edges of the DNA double-stranded break causing defective repair process. Nibrin also activates various signaling cascades such as; phosphatidylinositide 3-kinases PI3-kinase/Akt triggering the oncogenic process. Aims: The purpose of this research was to aid in the diagnosis of oral cancer and dysplastic lesions in Karachi population. Study Design: This cross-sectional study was conducted at a tertiary care hospital. All samples were recruited for the study after obtaining written informed consent from patients and seeking ethical approval from Ethic Review Committee Ziauddin University (Reference Code: 0330618FAOM), Karachi Pakistan. Place and Duration of Study: Ziauddin hospital north Nazimabad Karachi, November 2018 to September 2019. Methodology: Expression of Nibrin (NBS1) was evaluated by Immunohistochemistry on dysplasia and OSCC biopsies by H-Score method. The tumor was graded by broder system. Immunohistochemical analysis for nibrin was performed on the tumor block. Mean, frequency, percentages were calculated for quantitative variables, chi square was used for qualitative variables. A value <0.05 was considered as significant. Results: 92% OSCC cases were positive for Nibrin while 80% of oral dysplasia revealed Nibrin expression. This accounts for significant statistical association of Nibrin with OSCC (0.012*) and dysplasia (0.001*). Unfortunately, apart from moderaterly histological differentiation (0.012) none of these clinicopathological parameters showed statistical significance. Conclusion: We conclude that a Nibrin protein showed overexpression among 92% of OSCC patients out of total 100 cases and in 80% out of 30 cases of dysplasia so the association of histological grading with expression of Nibrin was significant and expression of Nibrin intensity with histological grading was significant as well. Our study suggests that this expression of Nibrin may be used as a diagnostic marker for potential OSCC patients.

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Intense orthodontic force induces the three dental pulp nitric oxide synthase isoforms and leads to orofacial discomfort in rats

Sobrinho

Aguiar

Machado

et al. 2022

Orthod Craniofacial Res

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Objective The objective of this study was to evaluate the role of nitric oxide synthase (NOS) isoforms influence during tooth movement with different forces. Settings and sample population 100 male Wistar rats (n = 10/group) were divided into a Sham group (animals not submitted to device installation nor Induced Toot Movement [ITM]), Negative Control Group (NCG) (animals submitted to device installation but not to ITM) and three experimental groups (F1, F2 and F3) (submitted to ITM with forces of 25, 50 and 100 gF respectively). Materials and methods A daily count of biting and scratching on the vibrissae and the Grimace scale were applied. After 4 (D4) and 11 (D11) days, the molar diastema was measured, and the animals were euthanized for histological (vascular parameters) and immunohistochemistry (iNOS, eNOS and nNOS) in the dental pulp. Results On D4, there was significant movement in the F3 group (P = .001) and on D11 in F1, F2 and F3 (P < .001). The number of bites (P < .001) and scratching (P = .006) was higher in F2‐F3, and F3 had higher Grimace scores (P < .001) and weight loss (P < .001). At D4, there was an increase in pulp ectasia in F2‐F3 (P = .021) and a reduction in the number of vessels in F3 (P = .005). In D4 and D11, there was a significant increase in immunostaining for iNOS and eNOS in F1 (P = .025 and P < .001 respectively) and F2 (P = .007 and P < .001 respectively). At D4, F2 and F3 showed higher immunostaining for nNOS (P = .027). Conclusion Thus, IDM induced inflammatory changes in the dental pulp reflecting in force‐dependent pain/suffering signs.

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Nibrin expression in oral squamous cell carcinoma: association with clinicopathological parameters

Cited by 7 publications

References 32 publications

Mediator of DNA damage checkpoint protein 1 (MDC1) as a prognostic marker for patients with oral squamous cell carcinoma

Mediator of DNA damage checkpoint protein 1 (MDC1) as a prognostic marker for patients with oral squamous cell carcinoma

Immunohistochemical Expression of Nibrin in Epithelial Dysplasia and OSCC: A Cross-Sectional Study

Intense orthodontic force induces the three dental pulp nitric oxide synthase isoforms and leads to orofacial discomfort in rats

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