NIAID workshop on T cell technologies

Gondré-Lewis, Timothy; Jiang, Chao; Ford, Mandy L.; Koelle, David M.; Sette, Alessandro; Shalek, Alex K.; Thomas, Paul G.

doi:10.1038/s41590-022-01377-x

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…The different approaches represent the fundamental tensions that exist between ease of performance, robustness, cost, and throughput on the one hand and physiological relevance, complexity, depth of analysis, and granularity of information on the other. A comprehensive discussion of the available techniques is beyond the scope of the present article, and some recent reviews have discussed the topic (Gondre‐Lewis et al., 2023; Peters et al., 2020; Sidney et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

The MegaPool Approach to Characterize Adaptive CD4+ and CD8+ T Cell Responses

da Silva Antunes,

Weiskopf,

Sidney

et al. 2023

Current Protocols

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Epitopes recognized by T cells are a collection of short peptide fragments derived from specific antigens or proteins. Immunological research to study T cell responses is hindered by the extreme degree of heterogeneity of epitope targets, which are usually derived from multiple antigens; within a given antigen, hundreds of different T cell epitopes can be recognized, differing from one individual to the next because T cell epitope recognition is restricted by the epitopes’ ability to bind to MHC molecules, which are extremely polymorphic in different individuals. Testing large pools encompassing hundreds of peptides is technically challenging because of logistical considerations regarding solvent‐induced toxicity. To address this issue, we developed the MegaPool (MP) approach based on sequential lyophilization of large numbers of peptides that can be used in a variety of assays to measure T cell responses, including ELISPOT, intracellular cytokine staining, and activation‐induced marker assays, and that has been validated in the study of infectious diseases, allergies, and autoimmunity. Here, we describe the procedures for generating and testing MPs, starting with peptide synthesis and lyophilization, as well as a step‐by‐step guide and recommendations for their handling and experimental usage. Overall, the MP approach is a powerful strategy for studying T cell responses and understanding the immune system's role in health and disease. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Generation of peptide pools (“MegaPools”)Basic Protocol 2: MegaPool testing and quantitation of antigen‐specific T cell responses

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Section: Introductionmentioning

confidence: 99%

The MegaPool Approach to Characterize Adaptive CD4+ and CD8+ T Cell Responses

da Silva Antunes,

Weiskopf,

Sidney

et al. 2023

Current Protocols

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An update on studies characterizing adaptive immune responses in SARS-CoV-2 infection and COVID-19 vaccination

Antunes

Grifoni

Weiskopf

et al. 2023

International Immunology

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In this brief opinion piece, we highlight our studies characterizing adaptive SARS-CoV-2 immune responses in infection and vaccination, and the ability of SARS-CoV-2-specific T cells to recognize emerging variants of concern, and the role of pre-existing cross-reactive T cells. In the context of the debate on correlates of protection, the pandemic's progression in the past three years underlined the need to consider how different adaptive immune responses might differentially contribute to protection from SARS-CoV-2 infection versus COVID-19 disease. Lastly, we discuss how cross-reactive T cell responses may be useful in generating a broad adaptive immunity, recognizing different variants and viral families. Considering vaccines with broadly conserved antigens could improve preparedness for future infectious disease outbreaks.

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NIAID workshop on T cell technologies

Cited by 2 publications

References 25 publications

The MegaPool Approach to Characterize Adaptive CD4+ and CD8+ T Cell Responses

The MegaPool Approach to Characterize Adaptive CD4+ and CD8+ T Cell Responses

An update on studies characterizing adaptive immune responses in SARS-CoV-2 infection and COVID-19 vaccination

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