Ni-functionalized submicron mesoporous silica particles as a sorbent for metal affinity chromatography

Kurdyukov, D. A.; Chernova, Ekaterina; Russkikh, Ya. V.; Eurov, D. A.; Соколов, В. В.; Bykova, Anna; Shilovskikh, Vladimir V.; Keltsieva, Olga A.; Ubyivovk, E. V.; Anufrikov, Yuri A.; Fedorova, Anna; Selyutin, A. A.; Суходолов, Н. Г.; Podolskaya, Е. P.; Голубев, В. Г.

doi:10.1016/j.chroma.2017.07.043

Cited by 9 publications

(4 citation statements)

References 24 publications

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“…S1) были сформированы один, два, четыре, шесть и восемь слоев FLa, в трех последних случаях вод- ную каплю удаляли и наносили заново после формирования каждых двух слоев. Во всех экспериментах в качестве водной субфазы использовали раствор LaCl 3 (C = 10 −2 mol/l, рН = 3, V = 0.7 µl), а в качестве органической -насыщенный при 20 [5,7]. Последующий фрагментный анализ также подтвердил эту структуру (см.…”

Section: поступило в редакцию 22 июля 2022 г в окончательной редакции...unclassified

“…Ранее нами была впервые продемонстрирована возможность экстракции хлорсодержащих органических соединений из образцов методом металл-аффинной хроматографии [7]. Соответственно представлялось интересным оценить специфичные и селективные свойства полученных мультимолекулярных структур FLa на примере аддуктов глобина человека с хлорсодержащими соединениями.…”

Section: поступило в редакцию 22 июля 2022 г в окончательной редакции...unclassified

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Формирование Наноразмерных Мультимолекулярных Структур Стеарата Лантана С Использованием Монослоев Ленгмюра Для Масс-Спектрометрии С Лазерной Десорбцией/Ионизацией

Горбунов¹,

Подольская²

2022

Письма В Журнал Технической Физики

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Исследована матрично-активированная лазерная десорбция/ионизация (МАЛДИ) с поверхности наноразмерных мультимолекулярных структур на основе монослоев стеарата лантана, наличие которых на поверхности подтверждалось экспериментально по лазерной десорбции лантансодержащих органических ионов. Показано, что такая функционализация поверхности стандартной МАЛДИ-мишени приводит к значительному увеличению выхода ионов целевых пептидов, причем оптимум достигается при толщине пленки порядка шести монослоев. Предложен подход в формате "Лаборатория на мишени", позволяющий проводить специфичную экстракцию пептидов, модифицированных хлорсодержащими соединениями, и включающий следующие стадии: функционализация поверхности мишени, металл-аффинная экстракция, нанесение матрицы, МАЛДИ масс-спектрометрический анализ. Ключевые слова: масс-спектрометрия, матрично-активированная лазерная десорбция/ионизация, поверхность, монослои Ленгмюра, металл-аффинная хроматография.

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Section: поступило в редакцию 22 июля 2022 г в окончательной редакции...unclassified

Формирование Наноразмерных Мультимолекулярных Структур Стеарата Лантана С Использованием Монослоев Ленгмюра Для Масс-Спектрометрии С Лазерной Десорбцией/Ионизацией

Горбунов¹,

Подольская²

2022

Письма В Журнал Технической Физики

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“…The latter technique is currently recognized as the "working horse" of phosphoproteomics, and in the absolute majority of cases relies on titanium dioxide (TiO 2 ) affinity chromatography [253], although the stationary phase can be represented by zirconia (salts of Zr 4+ ) [254], aluminum hydroxide [255] and iron oxide [222] as well. Recently, Ni-functionalized particles [256] and iron (III) stearate Langmuir-Blodgett films [222] were proposed as affinity materials for IMAC, also these techniques are still to be implemented in plant and seed biology. Pre-fractionation of complex protein digests might be another approach, giving access to enhanced phosphopeptide detection.…”

Section: Post-translational Modificationsmentioning

confidence: 99%

Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

Smolikova

Gorbach

Lukasheva

et al. 2020

IJMS

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For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins, and carbohydrates in their seeds, distinctly dominate modern agriculture, thus play an essential role in food industry and fuel production. Therefore, seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, seed development and germination as well as age- and stress-related alterations in seed vigor, longevity, nutritional value, and safety can be addressed by a broad panel of analytical, biochemical, and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes accompanying plant development, senescence, and response to biotic or abiotic stress. Among individual post-genomic methodological platforms, proteomics represents one of the most effective ones, giving access to cellular metabolism at the level of proteins. During the recent decades, multiple methodological advances were introduced in different branches of life science, although only some of them were established in seed proteomics so far. Therefore, here we discuss main methodological approaches already employed in seed proteomics, as well as those still waiting for implementation in this field of plant research, with a special emphasis on sample preparation, data acquisition, processing, and post-processing. Thereby, the overall goal of this review is to bring new methodologies emerging in different areas of proteomics research (clinical, food, ecological, microbial, and plant proteomics) to the broad society of seed biologists.

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“…Previously, we demonstrated for the first time the possibility of extracting chlorine-containing organic compounds from samples by metal-affinity chromatography [7]. Accordingly, it seemed interesting to evaluate the specific and selective properties of the obtained FLa multimolecular structures using the example of human globin adducts with chlorine-containing compounds.…”

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confidence: 99%

Fabrication of nanoscale multimolecular structures of lanthanum stearate using Langmuir monolayers for laser desorption/ionization mass spectrometry

Yu.

Podolskaya

2022

Technical Physics Letters

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Matrix-assisted laser desorption/ionization (MALDI) from the surface of nanosized multimolecular structures based on lanthanum stearate monolayers (FLa) has been studied. The presence of FLa on the surface of MALDI target was confirmed experimentally by laser desorption of lanthanum-containing organic ions. MALDI target functionalization with FLa is shown to significantly increase the yield of target peptide ions, with the optimum being achieved at a film thickness of about 6 monolayers. An approach is proposed in a "lab-on-a-plate" format, which allows specific extraction of peptides modified with chlorine-containing compounds and includes the following steps: functionalization of the target surface, metal affinity extraction, matrix deposition and MALDI mass spectrometric analysis. Keywords: mass spectrometry, matrix assisted laser desorption/ionization (MALDI), surface, Langmuir monolayers, metal affinity chromatography.

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Ni-functionalized submicron mesoporous silica particles as a sorbent for metal affinity chromatography

Cited by 9 publications

References 24 publications

Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

Fabrication of nanoscale multimolecular structures of lanthanum stearate using Langmuir monolayers for laser desorption/ionization mass spectrometry

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